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Citations for BioLegend :
3551 - 3600 of 4293 citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... followed by 30-min incubation with the following diluted antibodies: BV605-anti-mouse CD45 (BioLegend #103139, 1:200), BV421-anti-mouse Ly6G (BioLegend #127627 ...
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bioRxiv - Neuroscience 2024Quote: ... and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies, 1:12000 dilution; Biolegend, San Diego, CA) immunohistochemistry.
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bioRxiv - Neuroscience 2024Quote: Primary antibodies used in this study included mouse IgG2a-anti-β-III-tubulin (Tuj1, 1:400, #801202, Biolegend) to label SGNs ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor cells were washed with 1xPBS and blocked with 5% mouse Fc blocking reagent (Biolegend, San Diego, CA) in FACS buffer (1% FBS and 1 mM EDTA in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with TruStain FcX PLUS (anti-mouse CD16/32) Antibody (BioLegend, Cat#156603; 0.25 μg/sample) and Human TruStain FcX (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... all cells were blocked with 1:50 dilution of TruStain FcX anti-mouse CD16/CD32 (Clone 93, Biolegend) and Fixable Viability Dye (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was Alexa Fluor 555 goat anti-mouse IgG (minimal x- reactivity) (405324, P4U/BioLegend UK). For nuclear counterstaining ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Developmental Biology 2024Quote: ... 1:100) and Alexa Fluor 488 anti-mouse Ly-6A/E (Sca-1) antibody (108115, BioLegend, 1:1000) diluted in PBS ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: NK cells were defined as positive for CD56dim using Brilliant Violet 650 conjugated mouse anti-human CD56 (BioLegend) and PerCP/Cyanine5.5 conjugated mouse anti-human CD16 antibody (BioLegend ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: The anti-mouse antibody CD45-PE-Dazzle5 clone 30-F11 was purchased from BioLegend (San Diego, CA, USA) and used for flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... TOYOBO) supplemented with 50 μg/mL of FITC anti-mouse I-A/I-E (clone M5/114.15.2, BioLegend) for 1 h at room temperature in the dark following pretreatment with Ultra-LEAF purified anti-mouse CD16/32 Ab (clone 93 ...
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bioRxiv - Immunology 2024Quote: ... and 1 mL 400 mg/mL Pacific Blue-mouse-anti-human CD45 (BioLegend; Cat. No. 304029; clone HI30), 1 mL 50 mg/mL PE/Dazzle594-mouse-anti-human CD16 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and 1 mL 70 mg/mL BV421-mouse-anti-human CD103 (Biolegend; Cat. No. 350214; clone Ber-ACT8). After the incubation ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were used for flow cytometry: anti-mouse Thy1.2 Brilliant Violet 785 (clone 30-H12, BioLegend), anti-mouse Thy1.1 Brilliant Violet 421 (clone OX-7 ...
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated from the spleen using the MojoSortTM Mouse CD3 T cell Isolation Kit (Biolegend). Experiments where purified T cells were cultured for longer than 24 hours ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were quantified by a fluorescence-encoded bead-based multiplex assay (LEGENDplex Mouse Anti-Virus Response Panel, BioLegend), per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Grids were incubated with 1:1 anti CX43 antibody (mouse mAb, epitope aa 247-382; BioLegend Cat#850001) and 1:10 anti GIV antibody (Cat#ABT80 ...
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bioRxiv - Immunology 2023Quote: ... and plated in TRM-like culture media with 10 ng/mL mouse IL-7 (Biolegend Catalog No. 577804) and 10 ng/mL human TGFβ1 (Fisher Scientific PHG9214 ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells were magnetically isolated from the spleen and LNs of Rag2-/- TCR7B8 Tg or TCR1A2 Tg mice on CD45.2/CD45.2 background using the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend). Then ...
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bioRxiv - Immunology 2023Quote: ... The IcAR-PD1 cell line was stained using PE-conjugated mouse anti-human PD1 antibody (cat: 379210, BioLegend). PE-conjugated mouse IgG2b (cat ...
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bioRxiv - Immunology 2023Quote: ... and ST2 were determined by flow cytometric analyses using FITC-labeled anti-mouse FcεRIα (clone MAR-1, BioLegend), APC-labeled anti-mouse CD117 (clone 2B8 ...
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bioRxiv - Developmental Biology 2023Quote: TotalSeq™-B0301-306 anti-mouse Hashtag Antibodies (clone M1/42; 30-F11;, Biolegend, RRID: AB_ 2814067-2814072) were used to label individual colonies for multiplexing (Stoeckius et al. ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with FACS buffer and stained with goat-anti-mouse IgG-Alexa Fluor647 (1:400, Biolegend) in FACS buffer for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: Surface expression of mouse or human CD47 on murine tumor cell lines was assessed using MIAP301-PE (Biolegend) and CC2C6-APC (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... primary conjugated antibodies (AF647) were used directly for cell labeling and included mouse IgG1,k isotype (BioLegend, 400130), mouse IgG2b isotype (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse PD-L1 (10F.9G2 clone [BioXcell, Lebanon, NH, USA] or MIH6 clone [BioLegend, San Diego, CA, USA]), control mouse IgG ...
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bioRxiv - Immunology 2023Quote: ... All samples were blocked with Fc-block anti-mouse CD16/32 antibody (clone: S17011E, cat. no. 156603, BioLegend). Single-cell suspensions were stained 30min at 4°C in the dark with PerCP/Cyanine5.5 anti-mouse Ly6G (clone:1A8 ...
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bioRxiv - Microbiology 2023Quote: ... fluorescein isothiocyanate-conjugated polyclonal goat anti-human C3b antibody (ICN) and PE-conjugated goat anti-mouse IgG (Biolegend). For the neutrophil killing assays ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ T cell isolation kit (BioLegend) and cultivated as described above ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained with an allophycocyanin (APC)-anti-CD14 or APC-anti-mouse IgG1 isotype control antibody (BioLegend) on ice ...
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bioRxiv - Immunology 2023Quote: ... All samples were blocked with Fc-block anti-mouse CD16/32 antibody (clone: S17011E, cat. no. 156603, BioLegend). After the supernatant was discarded by centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Surface markers were subsequently stained on ice for 20 minutes: anti-mouse CD3-PE (1:200, 17A2, Biolegend), anti-mouse CD4-APC (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... and SMI-32 (using mouse Purified anti-Neurofilament H nonphosphorylated antibodies, 1:12000 dilution; Biolegend, San Diego, CA) immunohistochemistry (note that only sections adjacent to every 3rd Nissl-stained sections were processed for calbindin or SMI-32 ...
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bioRxiv - Immunology 2023Quote: ... freshly sorted cells were stained ex vivo or after stimulation with carrier-free recombinant mouse IL-25 (Biolegend) at a concentration of 100 ng/ml in complete RPMI medium for 36 h ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and detection antibodies (biotinylated mouse anti-IgG1, anti-IgG2a or anti-IgE from BioLegend) were added and incubated at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with mouse anti-HA.11 epitope tag antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) for 30 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... magnetic activated cell sorting (MACS) was applied using the mouse IgM BG-1 antibody (BioLegend, San Diego, CA) coupled to α-mouse IgM microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Dynabeads M270-Epoxy (Thermo) was covalently conjugated with combinations of mouse anti-human CD3 antibody (clone UCHT1, BioLegend), mouse anti-human CD28 antibody (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... and incubated for 30 minutes with TruStain FcX™ PLUS (anti-mouse CD16/32) antibody (BioLegend, Cat# 156604). Immunodetectable oxPLs were analyzed using custom-made PE-conjugated E06 rabbit antibody (Absolute antibody ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were pre-stained with Fc blocker for 10 min in ice followed by staining at 1:300 with anti-CD64 (139304, PE anti-mouse CD64, clone:X54-5/7.1, BioLegend) and anti-Pdgfra (25-1401-82 ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse CD279 (PD-1) (clone: RMP1-30, PE, Biolegend, cat# 109104; clone: RMP1-30, PE-Cy7, Biolegend, cat# 109110 ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with monoclonal antibodies anti-mouse (anti-CD45 APCcy7 [clone30-F11, Cat #103116, RRID:AB_312981], anti-CD3 BV605 [clone 172A, Biolegend, Cat #100237 ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were incubated in mouse Fc block (anti-CD16/32) antibody (Biolegend, 101335, 1:300) and then stained with the appropriate antibodies in FACS Buffer as described previously [33] ...
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bioRxiv - Bioengineering 2023Quote: Serum interferon alpha and beta were analyzed by ELISA (LEGEND MAX™ Mouse IFN-β ELISA Kit – BioLegend) (ELISA MAX™ Deluxe Set Mouse IFN-α1 – BioLegend).
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bioRxiv - Molecular Biology 2024Quote: ... incubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were incubated for 30min with the following antibodies in 1:100 concentration: APC anti-mouse CD45 (Biolegend), APC anti-mouse Ter119 (Biolegend) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain and spleen cells were stained with 1 µL from each FITC anti-mouse CD45 (Biolegend, Cat. 157214), PE anti-mouse CD11b (Biolegend ...