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Citations for BioLegend :
3551 - 3600 of 4288 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG antibody (10μg/mL, Biolegend, Cat No. 637301) was used instead ...
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bioRxiv - Immunology 2023Quote: ... soluble mCD28 (0.5 µg ml-1, clone 37.51, Biolegend), rhIL-2 (50 Uml-1 ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse CD40 (10 μg/mL, Clone:1C10, Biolegend), or LPS (10 μg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... and 100 ng per mL M-CSF (BioLegend, USA). For western blot analysis ...
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bioRxiv - Immunology 2024Quote: ... incubated with 1 ml erythrocyte lysis buffer (Biolegend, 420301) for 7 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... a-MBP AF594-conjugated (Biolegend, 850905, 0.5 mg/mL) diluted at 1/100 in TBS-T or with MAG (Bio-Techne ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Immunology 2021Quote: ... Slides were again washed 3 times with 1x TBS and stained with FITC–anti-IgD mAb (clone 11-26c.2a; 405713, BioLegend), PE goat-anti-mouse-IgM mAb (406507 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were washed once with 15 mL PBS and resuspended in 1 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 2 min at room temperature to lyse RBCs ...
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bioRxiv - Cell Biology 2021Quote: ... Immunostaining was performed with biotinylated monoclonal mouse antitubulin (clone 236-10501, Thermo, 1 μg/ml) then Brilliant Violet 421-conjugated streptavidin (Biolegend, 0.1 μg/ml), Alexa Fluor 488-conjugated mouse anti-perforin (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... T cells were added to anti-CD3 coated wells at 106 cells/mL in the presence of 1 μg/mL anti-CD28 (Biolegend, LEAF purified, 37.51), 100 U/ml IL-2 (gift from K ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained in 50 μl total volume for at least 30 min on ice with PE/Cy7 anti-mouse Ter119 (Biolegend, Cat# 116222) (1:50 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Dead cell compensation and gating was completed using a 50 % mixture of live and dead mESCs stained with Zombie Aqua™ Fixable Viability Kit (BioLegend 423101) and incubated in the light-tight container at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: Whole lung single cell suspensions were prepared as above and then blocked in SB containing 1:50 TruStain FcX™ (anti-mouse CD16/32) Antibody (BioLegend, #101319) for 10 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed twice with PBSF and labeled with 50 μL of APC-conjugated monoclonal mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, Cat # 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed together at 2x concentration in 50 μL and added directly to the cell suspension: BV605 CD45 (1:500, Biolegend, cat#103139), AF700 CD11b (1:300 ...
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bioRxiv - Biochemistry 2021Quote: ... plus 0.5 or 1 μg/ml αCD28 (BioLegend, catalog #302943), and returned to the incubator at 37°C ...
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bioRxiv - Immunology 2020Quote: ... CD122+BMDMs were generated by adding 1ng/mL IFNα (Biolegend) to the medium on day 5 of culture ...
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bioRxiv - Immunology 2022Quote: ... and 2μg/ml anti-IL4 (Biolegend, San Diego, CA, USA) for 3 days ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... with cold PBS containing Fc block (0.125 g/ml Biolegend) and antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by resuspension with 50ug/mL PI solution (421301, Biolegend). Stained cells were analyzed with BD LSRFortessa.
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bioRxiv - Genomics 2020Quote: ... and stimulated with 1 μg/mL anti-CD3 (BioLegend 300334) and 1 μg/mL anti-CD28 (BioLegend 302944 ...
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bioRxiv - Immunology 2021Quote: ... soluble CD28 antibody added (clone CD28.2 at 0.5μg/mL) (BioLegend). Other activation types assessed include ...
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bioRxiv - Cell Biology 2021Quote: ... APC anti-NKp30 (clone P30-15, Biolegend, 10 μg/ml) and FITC anti-CD18 (clone 6.7 ...
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bioRxiv - Immunology 2020Quote: ... for BMM generation or 10 ng/ml GM-CSF (Biolegend) for BMDC generation ...
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bioRxiv - Neuroscience 2020Quote: ... APC/Cyanine7 anti-mouse CD86 (1 µg/mL, Biolegend, USA), and PerCP/Cyanine 5.5 CD206 (5 ug/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml mouse EGF (BioLegend, San Diego, CA, USA), 10 µg/ml bovine insulin (Sigma-Aldrich by MilliporeSigma) ...
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bioRxiv - Physiology 2022Quote: ... and 20 ng/mL macrophage colony-stimulating factor (BioLegend, 576406). On the 4th and 6th day medium was changed to fresh ...
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bioRxiv - Immunology 2022Quote: ... and 30ng/ml of CD3 (clone 145-2C11, 100314, BioLegend) and 30ng/ml CD28 (clone 37.51 ...