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Citations for BioLegend :
3451 - 3500 of 8294 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Cancer Biology 2024Quote: ... Stimulated PBMCs were maintained in RPMI-1640 supplemented media with 100 ng/ml CD3 and 10 ng/ml recombinant human IL-2 (Biolegend, 589104).
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).
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bioRxiv - Immunology 2024Quote: ... single cell suspension was incubated with the cell hashtag antibodies (unique DNA barcodes listed in Supplementary Table 2) and the cocktail of 119 unique cell surface antibodies (Biolegend, #199901), as per manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... Cells were plated in non-TC treated 24-well plates previously coated with 2 µg/mL of anti-CD3 antibody (Biolegend, #317304) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin, PE) (BioLegend – 133906; 0.25 µg per million cells), rat anti-mouse CD31 (labelled with AF647 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were incubated for one hour at 4°C in the dark with AF647 rat anti-mouse CD326 (Epcam) antibody (BioLegend – 118212; 0.25 µg per million cells) in 200µl staining buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE) (BioLegend – 105808; 0.4 µg per million cells), rat anti-mouse CD34 (labelled with AF700 ...
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bioRxiv - Cell Biology 2019Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... from BD biosciences and APC-Cy7 anti-CD10 (1/100, #312212) PE anti-CD117 (1/100, #313210) from Biolegend.
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bioRxiv - Cancer Biology 2021Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... or SIV Gag or Env peptides (1 μg/mL) for 1 hour with CD107a PECy5 (eBioH4A3, BioLegend) in R10 media before adding 1 mg/mL of Brefeldin A (Sigma-Aldrich®) ...
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bioRxiv - Bioengineering 2019Quote: ... and Pacific Blue™ anti-Ly-6G/Ly-6C (Gr-1) (1:70, clone RB6-8C5, Biolegend).
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bioRxiv - Neuroscience 2019Quote: ... and cells were stained with rat anti-mouse ICAM-1 (1:100; Biolegend YN1/1.7.4 in PerCP), rat anti-mouse CD45 (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... CD40-APC (1:100) and CD11c-e450 (1:100) (all antibodies from Biolegend, San Diego, CA, USA). Cells were incubated with 100μl of the antibody suspension for 20 minutes at 4°C before a final wash in Ca2+/Mg2+ free PBS containing 2mM EDTA at x400g for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 5-10 µg/ml) or purified KPL-1 anti-PSGL-1 mAb (cat. no. 328802, Biolegend). The PL1 hybridoma developed by McEver ...
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bioRxiv - Immunology 2023Quote: ... T cell proliferation was stimulated with anti-CD3ε (1 μg/mL)/CD28 (1 μg/mL) antibodies (BioLegend). The anti-CD3ε antibody was coated on each well of the plate via overnight incubation at 4°C with PBS before coculture ...
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bioRxiv - Immunology 2023Quote: ... 1 × 106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa-Fluor®-488 SSEA1 (CD15) (1:50) and Alexa-Fluor®-647 SSEA5 (1:200) (BioLegend). In all experiments ...
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bioRxiv - Immunology 2023Quote: ... ST2 and CD13 was verified using FITC-coupled anti-FcεRI (1:100, clone MAR-1, BioLegend, #134306), PE-coupled anti-KIT (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies in 1:200 dilutions were used: CD31-PE-594 (1:200, Biolegend, Cat# 102520), CD45-APC (1:200 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with BV711 (1:50) or AlexaFluor647 (1:300) anti-mouse T-bet (Biolegend, 4B10), PE eFluor610 anti-mouse Eomes (Biolegend ...
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bioRxiv - Physiology 2024Quote: ... and immunofluorescence staining for PECAM-1 (AlexaFluor488 labelled primary monoclonal rat antibody, 5μg mL-1, MEC13.3, BioLegend) and S100A9 (Cy5.5 directly labelled ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated 20-60 minutes at room temperature and continued by adding 2 mL of 1X Permeabilization Buffer (Biolegend Cat. No. 421002) and centrifuged at 400-600 x g for 5 minutes at room temperature ...
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bioRxiv - Immunology 2019Quote: ... according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 μg/mL anti-human RP105 antibody clone MHR73-11 (BioLegend Cat.# 312907).
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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bioRxiv - Molecular Biology 2020Quote: ... The heme dose response was mixed 1:1 with 2X-concentrated antibodies conjugated to fluorescent dyes (FITC; Biolegend #100510 and eFluor780 ...
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bioRxiv - Immunology 2021Quote: ... 1×105-1×106 cells were stained after Fc-blocking with TruStain FcX (BioLegend, San Diego, CA, USA) and incubation with Zombie Aqua fixable viability dye (BioLegend ...
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bioRxiv - Cell Biology 2019Quote: ... APC-eFluor 780 CD25 (1:300; eBioscience, 47-0251-82) and Pe/Cy7 CD28 (1:200; Biolegend, 122014). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... then ILC2s were diluted 1:5 with PBS containing 1 μg/ml DAPI and Precision Count beads (BioLegend) were added to each sample ...
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bioRxiv - Cell Biology 2022Quote: ... and the fluorophore-conjugated antibody cocktail was added in 1:1 ratio (EpCAM, Clone 9C4, PE-Cy7, BioLegend, catalog number 324222 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for 1 hour before staining with APC-conjugated anti-BrdU antibody (Bu20a, Biolegend; 1:100) at room temperature (RT ...
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bioRxiv - Immunology 2020Quote: ... dil. 1:400), MHC class II (I-A/I-E) (M5/114.15.2; FITC, PerCP, dil. 1:800) (BioLegend). Data analysis was performed using FlowJo version 10 (TreeStar ...
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bioRxiv - Biochemistry 2022Quote: ... gels and western blotting using anti-FMRP (Cell signaling #LS-C82231, 1:500 or Biolegend #6B8, 1:1000), anti-GAPDH (Santa Cruz Biotechnology #sc-47724 ...
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bioRxiv - Immunology 2020Quote: ... 1:50 of anti-CD8 (FITC, RPA-T8) and 1:25 of anti-CD26 PE-Cy7 (BA5b, BioLegend), as well as IFN-γ ELISPOT assay to confirm MAIT cell identify and clonal purity.
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bioRxiv - Cell Biology 2020Quote: ... a cocktail mixture containing the following antibodies was used: (1) APC conjugated anti-mouse CD11b (1:200; BioLegend), CD31 (1:200 ...