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301 - 350 of 2921 citations for Recombinant Human REN His tagged since 2019

• Targeting Chromatin Effector Pygo2 to Enhance Immunotherapy in Prostate Cancer

  Yini Zhu, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and human CD8a (Biolegend, 372902).
• Identification of HIV-Reservoir Cells with Reduced Susceptibility to Antibody-Dependent Immune Response

  Antonio Astorga-Gamaza, et al., bioRxiv - Immunology 2022

  Quote: ... anti-human CD20 (BV785, Biolegend), and anti-human HLA-DR (BV711 ...
• Identification of HIV-Reservoir Cells with Reduced Susceptibility to Antibody-Dependent Immune Response

  Antonio Astorga-Gamaza, et al., bioRxiv - Immunology 2022

  Quote: ... anti-human CD3 (AF700, Biolegend), anti-human CD32 (PE-Cy7 ...
• B cells engineered to express an anti-HIV antibody allow memory retention, class switch recombination and clonal selection in mice

  Alessio D. Nahmad, et al., bioRxiv - Immunology 2020

  Quote: BV421 anti-human IgK (Biolegend)
• Potent Neutralizing Antibodies Directed to Multiple Epitopes on SARS-CoV-2 Spike

  Lihong Liu, et al., bioRxiv - Microbiology 2020

  Quote: ... and human Hashtag 3 (Biolegend) at 4°C for 1 hr ...
• Public and private human T cell clones respond differentially to HCMV antigen when boosted by CD3 co-potentiation

  Laura R.E. Becher, et al., bioRxiv - Immunology 2020

  Quote: ... anti-human CD45 (BioLegend, UCHL1), anti-human CD56 (BD Biosciences ...
• Public and private human T cell clones respond differentially to HCMV antigen when boosted by CD3 co-potentiation

  Laura R.E. Becher, et al., bioRxiv - Immunology 2020

  Quote: ... anti-human CD19 (BioLegend, HIB19), anti-Vβ5 (BioLegend ...
• Long-lasting germinal center responses to a priming immunization with continuous proliferation and somatic mutation

  Jeong Hyun Lee, et al., bioRxiv - Immunology 2021

  Quote: ... anti-human hashtag antibodies (Biolegend) were individually added to each sample at a concentration of 2.5 µg/ 5 million cells at the time of addition of the master mix ...
• MAFB surrogates the glucocorticoid receptor ability to induce tolerogenesis in dendritic cells

  Octavio Morante-Palacios, et al., bioRxiv - Immunology 2021

  Quote: ... and Human TNFα from BioLegend, and Human IL-1β from ThermoFisher.
• Disrupting CD38-driven T cell dysfunction restores sensitivity to cancer immunotherapy

  Or-Yam Revach, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Human TrueStain FcX (Biolegend, 422302) or mouse FcR blocker (Miltenyi 130-092-575 ...
• Metabolic Deficiencies Underlie Plasmacytoid Dendritic Cell Exhaustion After Viral Infection

  Trever T. Greene, et al., bioRxiv - Immunology 2024

  Quote: ... anti-human CD19 (HIB19, BioLegend), anti-human CD56 (MEM-188 ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-human CD19 (BioLegend, # 302212), anti-human CD33 (BioLegend ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-human CD33 (BioLegend, # 366608), anti-human CD34 (BioLegend ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-human CD235a (BioLegend, # 349104), anti-human CD19 (BioLegend ...
• Direct delivery of stabilized Cas-embedded base editors achieves efficient and accurate editing of clinically relevant targets

  Jeong Min Lee, et al., bioRxiv - Biochemistry 2024

  Quote: ... anti-human CD34 (BioLegend, # 343504), and anti-human CD3 (BioLegend ...
• Early human lung immune cell development and its role in epithelial cell fate

  Josephine L. Barnes, et al., bioRxiv - Immunology 2022

  Quote: ... using Human TruStain FcX (Biolegend) and then stained with FACS antibodies (Table S5) ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD4 (anti-human APC, Biolegend), and CD8 (anti-human PerCP-Cy5.5 ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD43 (anti-human PE, Biolegend), and CD45 (anti-human APC ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD11b (anti-human APC, Biolegend), CXCR3 (anti-human BV650 ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CXCR3 (anti-human BV650, Biolegend), CD45 (anti-human Alexa Fluor 700 ...
• HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model

  Alice K. Min, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD206 (anti-human BV785, Biolegend), and CD14 (anti-human APC Fire 750 ...
• B-Cell Precursor Acute Lymphoblastic Leukemia elicits an Interferon-α/β response in Bone Marrow-derived Mesenchymal Stroma

  Mandy W. E. Smeets, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PE anti-human CD45 (Biolegend), Alexa Fluor 750-conjugated human 5’-Nucleotidase/CD73 ...
• Activated interstitial macrophages are a predominant target of viral takeover and focus of inflammation in COVID-19 initiation in human lung

  Timothy Ting-Hsuan Wu, et al., bioRxiv - Genomics 2023

  Quote: ... anti-human CD45 (BioLegend 324016), CD204 (BioLegend 371904) ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... human IgM-AF700 (BioLegend, 314537), human IgD-perCP/Cy5.5 (BioLegend ...
• CD32 allows capturing blood cells emergence in slow motion during human embryonic development

  Rebecca Scarfò, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-human CD32 (Biolegend, 303202) and rabbit anti-human/mouse Runx1 (Abcam ...
• B-Cell Precursor Acute Lymphoblastic Leukemia elicits an Interferon-α/β response in Bone Marrow-derived Mesenchymal Stroma

  Mandy W. E. Smeets, et al., bioRxiv - Cancer Biology 2023

  Quote: ... BV650 anti-human CD16 (Biolegend), BUV395 anti-human CD19 and CD123 (BD Biosciences ...
• B-Cell Precursor Acute Lymphoblastic Leukemia elicits an Interferon-α/β response in Bone Marrow-derived Mesenchymal Stroma

  Mandy W. E. Smeets, et al., bioRxiv - Cancer Biology 2023

  Quote: ... BV605 anti-human CD45 (Biolegend), APC anti-human CD66b ...
• Targeted therapies prime oncogene-driven lung cancers for macrophage-mediated destruction

  Kyle Vaccaro, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or Human TruStain FcX (BioLegend). The following antibodies were used for FACS analysis ...
• SAP expressing T peripheral helper cells identify systemic lupus erythematosus patients with lupus nephritis

  Yevgeniya Gartshteyn, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human SLAMF6 (BioLegend #317202) and anti-human SLAMF3 (BioLegend #326109).
• SARS-CoV-2 spike protein induces the cytokine release syndrome by stimulating T cells to produce more IL-2

  Chao Niu, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human IL-6 (Biolegend), or isotype control antibodies for 30 min.
• Human pluripotent stem cell-derived respiratory airway progenitors generate alveolar epithelial cells and recapitulate features of idiopathic pulmonary fibrosis

  Mikael G. Pezet, et al., bioRxiv - Cell Biology 2023

  Quote: ... Conjugated human EPCAM antibody (Biolegend) was added for 30 minutes ...
• Ancestral SARS-CoV-2 immune imprinting persists on RBD but not NTD after sequential Omicron infections

  Zuowei Wang, et al., bioRxiv - Immunology 2024

  Quote: ... human IgM-AF700 (BioLegend, 314537), human IgD-perCP/Cy5.5 (BioLegend ...
• Broad-spectrum RNA antiviral inspired by ISG15^-/- deficiency

  Yemsratch T. Akalu, et al., bioRxiv - Immunology 2024

  Quote: ... human IFN-γ (Biolegend 43104) and human IFN-λ4 (AFG Bioscience EK716122VWR 76578-602).
• In vivo single cell transcriptomics reveals Klebsiella pneumoniae rewiring of lung macrophages to promote infection

  Amy Dumigan, et al., bioRxiv - Microbiology 2021

  Quote: THP-1 human macrophages were treated with mouse anti-human Fc block (Ref: 422302, BioLegend), for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend) ...
• Human immunocompetent Organ-on-Chip platforms allow safety profiling of tumor-targeted T-cell bispecific antibodies

  S. Jordan Kerns, et al., bioRxiv - Cancer Biology 2021

  Quote: ... which consisted of anti-human Alexa Fluor® 700 anti-human CD3 (BioLegend, cat. 300324), Brilliant Violet 785™ anti-human CD4 (BioLegend ...
• Overcoming Tumor Heterogeneity by Ex Vivo Arming of T Cells Using Multiple Bispecific Antibodies

  Jeong A Park, Nai-Kong V. Cheung, bioRxiv - Cancer Biology 2021

  Quote: ... Human Th1 cell released cytokines were analyzed by LEGENDplexTM Human Th1 Panel (Biolegend, Cat# 741035). Five human T cell cytokines including IL-2 ...
• Anti-thrombotic treatment enhances antibiotic efficiency in a humanized model of meningococcemia

  Jean-Philippe Corre, et al., bioRxiv - Pathology 2022

  Quote: ... human and murine Fc receptors were blocked using Human TruStain FcX (Biolegend, #422302, 1/20) and anti-mouse CD16/CD32 (FcBlock clone 2.4G2 ...
• Identification of a distinct cluster of GDF15^high macrophages exhibiting anti-inflammatory activities

  Chaochao Dai, et al., bioRxiv - Immunology 2024

  Quote: ... Human cells were blocked by Human TruStain FcX Fc receptor blocking solution (#422301 from BioLegend). Flow cytometry analysis was performed using an AccuriC6 Plus platform ...
• Dectin-1 limits central nervous system autoimmunity through a non-canonical pathway

  M. Elizabeth Deerhake, et al., bioRxiv - Immunology 2020

  Quote: ... Recombinant mouse (rm) M-CSF and GM-CSF proteins were obtained from BioLegend. MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK ...
• Metabolic regulation by prostaglandin E₂ impairs lung group 2 innate lymphoid cell responses

  Calum T. Robb, et al., bioRxiv - Immunology 2021

  Quote: ... Recombinant mouse IL-33 and Alternaria alternata (A.A) extract were purchased from Biolegend and Stallergenes Greer ...
• The IL-33-ILC2 pathway protects from amebic colitis

  Md Jashim Uddin, et al., bioRxiv - Immunology 2021

  Quote: Mice were administered 0.75 µg of recombinant IL-33 (BioLegend, San Diego, CA) or PBS via intraperitoneal injection daily beginning at 3 days before amebic challenge up until 1 day before harvest.
• IL-1α secreted by subcapsular sinus macrophages promotes melanoma metastasis in the sentinel lymph node by upregulating STAT3 signaling in the tumor

  Tommaso Virgilio, et al., bioRxiv - Immunology 2021

  Quote: ... B16-F1 and A375 cells were treated using carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304, BioLegend), and 10 μg/mL anti-mouse IL-12/23p40 antibody (clone C17.8 ...
• An mRNA-LNP-based Lassa virus vaccine induces protective immunity in mice

  Mei Hashizume, Ayako Takashima, Masaharu Iwasaki, bioRxiv - Microbiology 2023

  Quote: ... 10 µM each) and 50 U/mL of recombinant mouse IL-2 (BioLegend) overnight ...
• RNA editing of AZIN1 coding sites is catalyzed by ADAR1 p150 after splicing

  Yanfang Xing, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Raw 264.7 cells were cultured with 100 ng/mL recombinant mouse IFNβ1 (BioLegend). After 24 h ...
• Injectable butyrate-prodrug micelles induce long-acting immune modulation and suppress autoimmune arthritis in mice

  Shijie Cao, et al., bioRxiv - Bioengineering 2023

  Quote: ... GM-CSF (20 ng/mL; recombinant Mouse GM-CSF (carrier-free) from BioLegend) ...
• Suppression of autoimmune arthritis and neuroinflammation via an amino acid-conjugated butyrate prodrug with enhanced oral bioavailability

  Shijie Cao, et al., bioRxiv - Bioengineering 2023

  Quote: ... GM-CSF (20 ng/mL; recombinant Mouse GM-CSF (carrier-free) from BioLegend) ...
• Coenzyme A governs proinflammatory macrophage metabolism

  Greg. A. Timblin, et al., bioRxiv - Immunology 2024

  Quote: ... and 20ng/mL recombinant murine M-CSF (Shenandoah 200-08 or BioLegend 576404) in non-TC treated Petri dishes ...
• An mRNA SARS-CoV-2 vaccine employing Charge-Altering Releasable Transporters with a TLR-9 agonist induces neutralizing antibodies and T cell memory

  Ole A.W. Haabeth, et al., bioRxiv - Immunology 2021

  Quote: ... RBD was then detected using an Alexa Fluor 488 conjugated anti-His antibody (clone J099B1, Biolegend). Cells were analyzed on a flow cytometry (BD).
• SARS-CoV-2 multi-antigen protein microarray for detailed characterization of antibody responses in COVID-19 patients

  Alev Celikgil, et al., bioRxiv - Biochemistry 2022

  Quote: ... one slide was probed with Alexa Fluor® 647 anti-his antibody (1:250, BioLegend, 362611). Sera/plasma samples were single or serial diluted (three-fold dilutions from 1:90 ...