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Citations for BioLegend :
301 - 350 of 493 citations for Recombinant Canine CD274 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... XER mice were injected intraperitoneally at 1000h and 1800h on GD3 with either PBS or 10µg recombinant Lif (554006, BioLegend, San Diego, CA) followed by blue dye injection and dissection at GD4 1200h ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Immunology 2023Quote: Biotinylated S1 protein was obtained commercially (BioLegend). A 4X molar excess was incubated with streptavidin-Alexa Fluor 647 (0.5 mg/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Biochemistry 2022Quote: Moles of product formed in hydrolysis reactions used for ACE2 activity and specificity profiling were determined by comparing area under the curve for LC chromatograms corresponding to these reactions to areas under the curve observed for control reactions in which > 95% input substrate conversion was achieved by incubation of substrate with 25 nM recombinant human ACE2 commercial standard (BioLegend, San Diego, CA) for between one and four hours pending peptide substrate ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse IL4 (10 µg/mL) and recombinant mouse IL2 (5 ng/mL) and IL12 (10 ng/mL) (BioLegend, San Diego, CA, US), for three days prior to use.
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bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Immunology 2021Quote: ... Protein tyrosine phosphorylation was detected by PY20 (Biolegend) or 4G10 (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... myelin basic protein (mouse monoclonal, BioLegend, Dedham, MA), PDGFRα (goat polyclonal ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated SARS-CoV-2-S1 Protein (Biolegend 793804) Anti-Biotin (409003) ...
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bioRxiv - Immunology 2023Quote: ... RBD protein conjugated with PE-streptavidin (BioLegend, 405204) and APC-streptavidin (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and resulting cells were cultured in complete RPMI-1640 medium containing various concentrations of recombinant murine GM-CSF (rGM-CSF, BioLegend, San Diego, CA, USA), GPI-GM-CSF ...
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bioRxiv - Immunology 2019Quote: Mice were intranasally dosed with 3 µg in 20 µl per mouse recombinant murine TNF-α (r-TNF-α, carrier-free, 575204, BioLegend Ltd, UK) every day for 3 days ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Molecular Biology 2020Quote: ... or proteins (APC; Biolegend #103012 and PE; Biolegend #100206) prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner) ...
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bioRxiv - Molecular Biology 2020Quote: ... or proteins (APC; Biolegend #103012 and PE; Biolegend #100206) prepared as 2 μg/ mL solutions in black-bottom 96-well plates (Greiner) ...
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bioRxiv - Immunology 2021Quote: ... Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend). SIINFEKL epitope-specific T cells were studied using APC-labelled H-2Kb/SIINFEKL pentamer (F093-84B-E ...
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bioRxiv - Cancer Biology 2021Quote: ... Peridinin-Chlorophyll-Protein (PerCP) anti-mouse CD19 (115531, BioLegend) and PE-Cyanine 7 anti-mouse CD4 (25-0041-82 eBioscience) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cultures collected at different time points were subjected to TCA protein extraction (see Protein extraction by TCA) and immunoblotted with anti-Rpb1 (BioLegend 664912 clone 8WG16) or anti-Pgk1 (Abcam ab113687 clone 22C5D8 ...
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bioRxiv - Microbiology 2020Quote: ... in the presence of protein transport inhibitor (monensin 1000x, Biolegend). For antigen-specific study ...
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bioRxiv - Immunology 2022Quote: ... Peridinin chlorophyll protein complex (PerCP) Cy5.5-conjugated: CD4 (Biolegend, 344608), CD26 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations were measured using ELISA MAX Deluxe Sets (Biolegend; IL1B cat ...
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bioRxiv - Immunology 2023Quote: ... in the presence of protein transport inhibitor (monensin 1000x, Biolegend). Cells were blocked with anti-CD16/CD32 antibody (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection using anti-HA (Covance 16B12, BioLegend, Dedham, MA, USA) or antiactin antibodies (Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... expanded and screened for IGF2R protein by anti-IGF2R antibody (Biolegend) by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: Protein expression measurements were performed using TotalSeq-A reagents from Biolegend according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Bound biotinylated protein was detected by adding Avidin-HRP (405103, BioLegend) diluted 1:2000 in TSM buffer ...
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bioRxiv - Immunology 2020Quote: ... Bound proteins were detected by staining with fluorescently conjugated antibodies (BioLegend) and measured by flow cytometry ...
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bioRxiv - Biochemistry 2021Quote: ... USA)-conjugated anti-mouse CD206 and activated protein C (APC, BioLegend, USA)-conjugated anti-mouse CD86 at room temperature for 1 h without light ...
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bioRxiv - Genomics 2022Quote: Antibody-derived DNA tags for membrane proteins were purchased from Biolegend and listed in Table S5 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on blots were detected using anti-RpoB (Biolegend; 663905; AB_2566583), anti-RecA (Pocono Rabbit Farm & Laboratory ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... p38 mitogen-activated protein kinase (p38MAPK, mouse, 1:2000, Biolegend 602651), and phosphorylated-p38MAPK (phospho-p38MAPK ...
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bioRxiv - Immunology 2024Quote: ... peridinin chlorophyll protein-cyanin 5.5 (PerCP-Cy5.5)-anti-CD16 (3G8; BioLegend), Pacific Blue-anti-CD45 (HI30 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Secreted proteins were measured using the ELISA MAX Deluxe kits (Biolegend). Absorbance was measured at 450 nm and 570 nm using a Synergy H1 plate reader (BioTek) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-dephosphorylated neurofilament protein (clone smi32; Biolegend, San Diego, CA, USA); rabbit anti-Caspr1 (clone EPR7828 ...
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bioRxiv - Immunology 2022Quote: ... −phycoerythrin (PE) and −peridinin chlorophyll protein complex (PerCP) were purchased from Biolegend. Phospho-specific mAbs against p65 (S536 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies against the following proteins and epitopes were used: HA (16B12, BioLegend), β-actin (AC-74 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Genomics 2022Quote: ... Proteins of interest were visualized using an anti-Flag-PE (BioLegend, 637310), an anti-HA-Alexa647 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...
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bioRxiv - Immunology 2023Quote: ... WT biotinylated RBD protein conjugated with TotalSeq™-C0973 Streptavidin (BioLegend, 405275) and TotalSeq™-C0974 Streptavidin (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: The scFc protein was labeled with SNAP-Surface 488 (BG-488, Biolegend). In brief ...
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bioRxiv - Systems Biology 2021Quote: ... surface proteins were stained with a cocktail of 53 TotalSeq-C antibodies (Biolegend). Antibody concentrations were either predetermined by titration (Kotliarov et al. ...
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bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following cell surface proteins were purchased from Biolegend with different fluorochrome labels ...
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bioRxiv - Microbiology 2020Quote: ... Protein G agorose beads that were pre-adsorbed to either anti-HA (BioLegend) or anti-FLAG (Millipore ...