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Citations for BioLegend :
301 - 350 of 3686 citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 150.000 cells were stained with anti-DYKDDDK(Flag)-tag antibody (BioLegend, 637315) or isotype control for 30 min at 4°C and washed three times.
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bioRxiv - Immunology 2023Quote: ... One aliquot was stained with 10 μM Tag-it Violet (BioLegend, #425101) diluted in HBSS for 5 minutes on ice at a cell concentration of 1 million cells per milliliter ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-HA.11 Epitope tag clone 16B12 (BioLegend Cat# 901503, RRID:AB_2565005), mouse anti-HA-HRP clone 6E2 (Cell Signaling Technology Cat# 2999S ...
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bioRxiv - Neuroscience 2023Quote: ... Polyribosomes were immunoprecipitated (IP) with Anti-HA.11 Epitope Tag antibody (BioLegend) plus protein A/G magnetic beads ...
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bioRxiv - Microbiology 2020Quote: ... and β subunit of RNAP 1 in 10,000 (E. coli RNA Polymerase beta Monoclonal Antibody, BioLegend). Primary antibodies were incubated overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Systems Biology 2021Quote: ... deeply anesthetized mice (5% isoflurane) were retro-orbitally injected with 3 μg of anti-CD45 antibody (PerCP, clone 30-F11, Biolegend) 3 min prior to euthanasia [46,81] ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 647 anti-human human CD3 Antibody (BioLegend, 300416), Biotin anti-human CD3 Antibody (BioLegend ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were used for these studies: anti-human CD47-APC (1µl/106 cells in 100µl; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3), anti-human CD47-FITC (5µl/106 cells in 100µl ...
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bioRxiv - Genomics 2020Quote: ... The cells were centrifuged at 300xg for 5 minutes at room temperature and resuspended in 85 ul of staining buffer and incubated with 5 ul of Human TruStain FcX(Biolegend cat: 422301) for 10 minutes on ice in 5 ml FACS tubes ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tag monoclonal antibody (901513) was from BioLegend (previously Covance catalog# MMS-101R). HA-tag polyclonal Antibody (631207 ...
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bioRxiv - Biophysics 2023Quote: ... and labeled with AlexaFluor647-labeled anti-HA.11 Epitope Tag antibody (BioLegend, #16B12) at 5 μg/ml for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Immunology 2021Quote: ... Human Fc receptors (FcRs) were blocked using Human TruStain FcX™ (BioLegend). In samples stained with multiple BD Horizon Brilliant reagents ...
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bioRxiv - Cancer Biology 2023Quote: Human PBMCs were treated with Fc blocking solution (Human TruStain FcX, Biolegend) and stained with the following fluorescent antibodies from Biolegend (unless otherwise stated) ...
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bioRxiv - Immunology 2021Quote: ... human CD4-APC (Biolegend), human FoxP3-APC (eBioscience) ...
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bioRxiv - Neuroscience 2021Quote: ... Human TruStain FcXTM (Biolegend). Protected from light ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD45 (BioLegend #304014), human CD25 (BioLegend #302606) ...
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bioRxiv - Cancer Biology 2023Quote: ... human FOXP3 (BioLegend #320116), Alexa Fluor® 647-conjugated anti-mouse integrin αvβ5 Ab (clone ALULA ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD45 (BioLegend #304014), Zombie NIR™ Fixable Viability Kit (BioLegend #423106 ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD25 (BioLegend #302606), human FOXP3 (BioLegend #320116) ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD8 (BioLegend #344712), human CD45 (BioLegend #304014) ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD3 (BioLegend, #317324), mouse CD45 (BioLegend #103130) ...
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bioRxiv - Immunology 2024Quote: ... Human TruStain FcX (BioLegend) was added at 1:20 to block Fc receptors for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... human TGFβ1 (580706; BioLegend), human IL-12 (219-IL;R&D Sys-tems) ...
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bioRxiv - Bioengineering 2024Quote: ... Recombinant human FcγIIa (Biolegend), FcγRIIb (Biolegend) ...
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bioRxiv - Pathology 2019Quote: ... IL-9 and IL-6 determinations (Human IL-4, IL-5, IL-9 and IL-6 MAX, Biolegend, San Diego, CA 92121 USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Primary human neutrophils isolated as described were stained at room temperature for 20 minutes with 5 μM Calcein AM (BioLegend, Cat. No. 425201) and applied to the top well of a fluorescence-blocking 24-well transwell plate with 3 μm pores (Corning ...
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bioRxiv - Microbiology 2024Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-bodies used in this study included a monoclonal α-his-antibody (BioLegend, 652502) and polyclonal α-CsgA-antibody and α-TasA-antibody.
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bioRxiv - Immunology 2019Quote: All cytokine stimulations were performed as indicated with Interferon-alpha 2b (Intron-A) or Interferon-gamma (Biolegend, 570202) in complete DMEM ...
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bioRxiv - Immunology 2022Quote: ... Enriched LPLs from mice were stained with the following monoclonal antibodies: TCR-beta-BV510 (clone H57-597, BioLegend), CD4-BV711 (clone RMA4-5 ...