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Citations for BioLegend :
301 - 350 of 3592 citations for Human Rh Blood Group D Antigen RHD CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and a red blood cell lysis buffer (BioLegend, San Diego, CA) was added to remove any RBCs ...
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bioRxiv - Cell Biology 2023Quote: ... Red blood cells (RBC) were lysed with RBC Lysis Buffer (BioLegend) as described elsewhere 56 ...
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bioRxiv - Physiology 2023Quote: ... After lysing red blood cells by 1x RBC Lysis Buffer (BioLegend), cells were incubated at room temperature for 20 minutes with Zombie Aqua™ Fixable Viability dye (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... The pellet was resuspended in red blood cell lysis buffer (BioLegend), incubated for 4 mins at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse blood was treated with 1X RBC lysis buffer (Biolegend 420301). White blood cells were stained with CD3 ...
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bioRxiv - Immunology 2023Quote: ... followed by red blood cells (RBC) lysis (RBC lysis buffer, Biolegend). Cells were stained with the following lineage (Lin)-specific antibodies (all antibodies from BioXcell ...
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bioRxiv - Immunology 2023Quote: ... Erythrocytes were removed using the red blood cells lysis buffer (Biolegend). Cells were washed once and resuspended in FACS buffer for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... Red blood cell depletion was made with lysis buffer (BioLegend #420301) according to manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2024Quote: ... All cells were treated with Red Blood Cell Lysis buffer (Biolegend) and suspensions were filtered through 70 μm Nitex nylon mesh before further use.
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bioRxiv - Immunology 2023Quote: ... whole blood was directly stained with the following antibodies from BioLegend: C5aR (Cat# 344304 ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper) with hashtag 5 (BioLegend, Cat# 155809). The five cell populations were mixed and barcoded ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Immunology 2023Quote: ... both endometriosis and sham mice were treated with intraperitoneal (i.p.) injections of 1μg of rmIL-23 (n=6/group; 589006, BioLegend, USA), or PBS for controls (n=6/group) ...
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bioRxiv - Microbiology 2021Quote: THP-1 human macrophages were treated with mouse anti-human Fc block (Ref: 422302, BioLegend), for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... which consisted of anti-human Alexa Fluor® 700 anti-human CD3 (BioLegend, cat. 300324), Brilliant Violet 785™ anti-human CD4 (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Th1 cell released cytokines were analyzed by LEGENDplexTM Human Th1 Panel (Biolegend, Cat# 741035). Five human T cell cytokines including IL-2 ...
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bioRxiv - Pathology 2022Quote: ... human and murine Fc receptors were blocked using Human TruStain FcX (Biolegend, #422302, 1/20) and anti-mouse CD16/CD32 (FcBlock clone 2.4G2 ...
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bioRxiv - Immunology 2024Quote: ... Human cells were blocked by Human TruStain FcX Fc receptor blocking solution (#422301 from BioLegend). Flow cytometry analysis was performed using an AccuriC6 Plus platform ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (R&D) and IL12B (Biolegend) ELISAs were preformed to manufacturers instructions
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).
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bioRxiv - Immunology 2019Quote: ... The presence of human GM-CSF in the supernatant of eMSCs co-cultures with CD34+ HSPCs was assessed by enzyme-linked immunosorbent assay (ELISA) using the human GM-CSF ELISA MAX kit (Biolegend) as per manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T lymphocytes were purified (> 98%) by negative selection with MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3 ...
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bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytokines in frozen cell culture supernatant or frozen plasma were analyzed with a customized multiplex human or mouse cytokine Legendplex kit (BioLegend). Samples were measured at the MACS Quant Analyzer10 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... Comprehensive surface immunophenotyping was performed using LEGENDScreen Human PE Kit containing 371 pre-titrated antibodies and 354 unique specificities (BioLegend). The experimental run was multiplexed using GFP+ NCI-H358 cells resistant to sotorasib combined with non-fluorescent ...
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Bioengineering 2023Quote: IFNψ was measured from the supernatants of the co-culture experiments (TCE / CAR) using the colorimetric ELISA MAX Standard Set Human IFNψ kit (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were collected from effector-target cell co-cultures and analyzed for IFNy using either ELISA MAX Deluxe Human IFNγ kit (BioLegend) or Human IFNγ DuoSet ELISA (R&D Systems ...
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bioRxiv - Immunology 2023Quote: Cytokines secreted in the culture supernatant of cytokine-stimulated sip-T were measured by a LEGENDplex Human CD8/NK Panel kit (BioLegend) following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: NK cells were isolated from frozen PBMCs by negative selection using the MojoSort™ Human NK Cell Isolation Kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Primary NK cells from lineage-depleted apheresis or NK92 cells were incubated on cells or cell-free matrix for 30 minutes in the presence of directly conjugated antibodies to surface antigens CD56 (1:50, clone HCD56, Biolegend) and CD29 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspension was stained with the following antibodies directed against cell surface antigens for 30 minutes at 4°C: CD45::BV421 (H130, BioLegend) and HLA-DR::PE-Cy7 (L243 ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2020Quote: ... The following surface antigens were detected by the indicated mAb clone: B220 (RA3-6B2-BV785 and RA3-6B2-BV421, BioLegend), CD21 (7EG-PerCP-Cy5.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Except for immunohistochemistry for SOX-9 in which a β-gal staining was performed at 37°C for 3h followed by fixation with PFA 4% for 30 min at RT and incubation with unmasking antigen solution (927901; Biolegend). Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40 ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Cell Biology 2019Quote: Pellet cells obtained from BAL (n= 5 mice/group in three different experiment) were incubated with a mix of conjugated antibodies (Biolegend) for the following T-cell subset superficial markers ...
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bioRxiv - Immunology 2022Quote: ... Cells within each experimental donor group were pooled and labeled with green and red CFSE respectively according to manufacturer protocol (Biolegend). Cells were counted and mixed to produce a 1:1 ratio of green and red labeled cells ...
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bioRxiv - Immunology 2021Quote: ... The Netherlands and anti-human CD14-FITC (RRID:AB_830677) and anti-human CD163-AF647 (RRID:AB_2563475) from BioLegend, San Diego ...