Labshake search
Citations for BioLegend :
301 - 350 of 587 citations for Hepatitis E Virus Capsid Protein ORF2 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... Expression was validated by flow cytometry using Alexa Fluor 647-conjugated anti-DYKDDDDK tag antibody (BioLegend #637315).
-
bioRxiv - Cell Biology 2023Quote: ... Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse, Biolegend cat ...
-
bioRxiv - Synthetic Biology 2023Quote: ... stable pools of cells were analyzed by flow cytometry using APC anti-Flag-tag antibody (Biolegend; 637307). Stable pools went through another round of selection in complete media supplemented with with 0.7ug/mL puromycin and 1ng/mL human prolactin or purified mouse prolactin and were analyzed by flow cytometry ...
-
bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
-
bioRxiv - Immunology 2022Quote: ... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... a multiplex cytokine quantification (LEGENDplex HU Anti-Virus Response Panel (13-plex) and LEGENDplex HU Th Cytokine Panel (13-plex) (cat# 740390 and cat# 740722, BioLegend) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... and was analyzed with the DropArray (Curiox Biosystems) and the MAGPIX System (Luminex) or with LEGENDPlex Human Anti-Virus Response Panel (13-plex, BioLegend) and was analyzed with LSRII (BD ...
-
bioRxiv - Immunology 2023Quote: ... Binding of anti-TIGIT antibodies HB0036/HB0030 to cell surface was detected by APC-anti his (Biolegend/362605). For ELISA based assay of anti-TIGIT antibodies in tumour digest ...
-
bioRxiv - Immunology 2019Quote: Antibodies used for FACS surface and intracellular staining were as follows: IA/E-PE (Biolegend, M5/114.15.2), Bst-2-biotin (in-house conjugated ...
-
bioRxiv - Developmental Biology 2021Quote: ... rat anti-mouse Ly-6A/E (Sca1) (labelled with BV510) (BioLegend – 108129; 5 µl per million cells) and rat anti-lineage cocktail (labelled with BV421 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with CSM before pooling and staining with E-Cadherin-AF647 (clone 67A4, Biolegend) and EpCAM-FITC (clone 9C4 ...
-
bioRxiv - Immunology 2022Quote: ... and Alexa Fluor 647-anti mouse/human CD324 (E-cadherin) (clone DECAMA-1) was obtained from Biolegend.
-
bioRxiv - Cancer Biology 2023Quote: ... whereas the second allowed myeloid cell characterization with anti-mouse I-A/I-E (M5/114.15.2; Biolegend), CD11b (M1/70 ...
-
bioRxiv - Immunology 2022Quote: ... Proliferation was determined by labeling T cells with the Tag-IT Violet Proliferation Cell Tracking Dye (Biolegend, #425101), according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Washed cells were stained with two fluorescently labeled epitope tag-specific antibodies (anti-FLAG PE, Biolegend, cat# 637309), anti-HA Alexa 647 ...
-
bioRxiv - Cancer Biology 2023Quote: ... transgenic T cells were labeled with Tag-it Violet Proliferation and Cell Tracking Dye (Cat No. 425101 Biolegend). The frequency of SMARTA cells that proliferated during culture was determined as described by Gett et al ...
-
bioRxiv - Microbiology 2023Quote: ... gonorrhoeae (1 x 108 CFU) was incubated with 1.5 µl Tag-IT® Violet (TIV) (Biolegend, Catalog # 425101) in 100 µl 1X PBS + 5 mM MgSO4 ...
-
bioRxiv - Immunology 2023Quote: ... or MCC:I-Ek (cognate) tethered pMHCII complexes were labeled with Tag-it Violet according to the manufacturer’s instructions (BioLegend). M12 cells and 58α-β- cells were then chilled on ice for 30 minutes ...
-
bioRxiv - Developmental Biology 2023Quote: ... The grids were incubated with mouse anti-HA.11 epitope tag antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) for 30 min at RT ...
-
bioRxiv - Immunology 2023Quote: ... for 15 minutes at 4°C followed by incubation with AntiHA.11 Epitope Tag Affinity Matrix (BioLegend 900801) for 4 hours at 4°C under constant rotation ...
-
bioRxiv - Developmental Biology 2023Quote: ... Western blot analysis was performed using anti-HA.11 epitope tag antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006), anti-FLAG (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2019Quote: ... After transfection cell supernatants were collected and subjected to cytokine production analysis using LEGENDplex™ Mouse Anti-Virus Response Panel (13-plex) (BioLegend) on BD FACS Canto II flow cytometer using BD FACSDiva 8.0 software for acquisition and BioLegend’s LEGENDplex™ Data Analysis Software for data analysis.
-
bioRxiv - Immunology 2021Quote: ... Bound ligand was detected with a horseradish peroxidase (HRP)-conjugated anti-His antibody (652504, BioLegend, San Diego, CA, USA). After incubation for 1 hour at room temperature and washing ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a further 1 h incubation with anti-His HRP conjugate (BioLegend; San Diego, CA, USA; cat. no. 652504) diluted (1:2500 ...
-
bioRxiv - Immunology 2023Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
-
bioRxiv - Immunology 2022Quote: ... with PE-conjugated anti-mouse H-2 or I-A/I-E Ab and corresponding isotype controls (BioLegend) (for the MHC study) ...
-
bioRxiv - Immunology 2022Quote: ... HIV-infected cells were previously incubated with 10 μg/ml of anti-human HLA-E (clone 3D12, Biolegend) antibody for 20 mins at RT ...
-
bioRxiv - Immunology 2020Quote: ... dil. 1:400), MHC class II (I-A/I-E) (M5/114.15.2; FITC, PerCP, dil. 1:800) (BioLegend). Data analysis was performed using FlowJo version 10 (TreeStar ...
-
bioRxiv - Cancer Biology 2020Quote: ... anti-Phospho-c-Jun (Ser73) (D47G9) (Cell Signalling, #3270) and Ly-6A/E (Sca-1) (Biolegend, cat#108101). Appropriate Alexa Fluor labelled secondary antibodies (ThermoFischer Scientific ...
-
bioRxiv - Immunology 2022Quote: ... Cell surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with 2.5 μl of PE anti-human E-cadherin (50 μg/ml; Biolegend, 324106) or matched concentration of PE Mouse IgG1 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1:100) and Alexa Fluor 488 anti-mouse Ly-6A/E (Sca-1) antibody (108115, BioLegend, 1:1000) diluted in PBS ...
-
bioRxiv - Immunology 2024Quote: ... TOYOBO) supplemented with 50 μg/mL of FITC anti-mouse I-A/I-E (clone M5/114.15.2, BioLegend) for 1 h at room temperature in the dark following pretreatment with Ultra-LEAF purified anti-mouse CD16/32 Ab (clone 93 ...
-
bioRxiv - Immunology 2024Quote: ... Surface MHC class II and CD86 on BMDCs were stained with anti-I-A/I-E-PerCP (clone M5/114.15.2, BioLegend) and anti-CD86-PE (clone GL-1 ...
-
bioRxiv - Microbiology 2019Quote: ... SERINC5 levels were assessed via C-terminal HA tag using the mouse monoclonal HA.11 (Biolegend, San Diego, CA) at 1 μg/ml in Odyssey blocking buffer (LI-COR Biotechnology ...
-
bioRxiv - Genomics 2022Quote: ... Cells were then resuspended in FACS buffer and incubated with a BV-421 anti-DYDDDDK epitope tag antibody (BioLegend) for 1 hr at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... IP of HA-positive ribosomes was performed by incubating with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
-
bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... IFN-α and IL-6 were detected by the Mouse Anti-Virus Response Panel (13-plex) (Cat# 740622 BioLegend, San Diego, CA) and analyzed on the NovoCyte Flow Cytometer (Agilent ...
-
bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
-
bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
-
bioRxiv - Immunology 2023Quote: ... Soluble His6-tagged M6G at varying concentrations was incubated for 10 min with HRP-conjugated mouse anti-His antibody (BioLegend) diluted 1:2000 in 0.1% BSA/TBST ...
-
bioRxiv - Immunology 2023Quote: ... Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST, BioLegend).
-
bioRxiv - Immunology 2021Quote: ... then stained with the I-A/E-APC antibody (MHCII) (1:1,200) (eBioscience) and positively selected for I-A/E-positive cells using anti-APC Microbeads (Biolegend). The obtained final cell fraction consisted of total mTECs (CD45-EpCAM+Ly51-I-A/E+).
-
bioRxiv - Biophysics 2020Quote: ... The membrane was probed with an anti-Aβ antibody targeted to residues 1-16 (1:1000, #E 610, Biolegend) and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000 ...
-
bioRxiv - Immunology 2020Quote: ... stimulated library was stained for its respective marker with the following antibody: MHCII (APC anti-mouse I-A/I-E Antibody, Clone M5/114.15.2 Biolegend 107613), CD40 (APC anti-mouse CD40 Antibody ...
-
bioRxiv - Cancer Biology 2023Quote: ... immunohistochemistry (IHC) and flow-cytometry (FC): Pacific Blue anti-mouse Ly-6A/E (Biolegend, Cat#108120, 1:50 FC), Sca-1/Ly6A/E (E13 161-7 ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
-
bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.