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Citations for BioLegend :
301 - 350 of 7910 citations for 1 Butylpyrrolidin 1 ium 3 yl 2 cyclopentyl 2 hydroxy 2 phenylacetatechloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Vimentin (Santa-Cruz; 2 μg/mL) and mouse anti-placental alkaline phosphatase (Biolegend; 1 μg/mL). Immunostaining for hCMV was performed as previously described [32] ...
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bioRxiv - Genomics 2024Quote: ... and resuspended in 50ul PBS plus 2% FBS containing 1:100 diluted anti-CD69-APC antibody (BioLegend 310909). Cells were stained in the dark on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended at 2×106 in 100μl PBS with 2% FBS and incubated with Zombie violet fixable viability dye (Biolegend) and an antibody cocktail for 25min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2024Quote: ... for the clone that binds to the high affinity IL-2 receptor (CD25): we incubated 0.5ugr mouse Il-2 (PepreoTech) to 5ugr of an IL-2 mAb (JES6-1A12, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... The amount of IL-2 in the culture medium was measured using a mouse IL-2 ELISA kit (Biolegend).
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bioRxiv - Bioengineering 2024Quote: ... APC- CD81 (clone Eat-2; 2 µg/mL) detection antibodies or isotypic controls (all antibodies were purchased from BioLegend). Flow cytometry was performed on a MACSQuant 10 instrument (Miltenyi Biotech ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Immunology 2024Quote: ... human PBMCs were preincubated for 30 min with 2 μg/mL anti-human IL-12 (clone 24910; BioTechne) or 2 μg/mL mouse IgG1 (clone MOPC-21; BioLegend). In functional experiments ...
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bioRxiv - Immunology 2024Quote: ... cells were supplemented with 25 µl/mL ImmunoCult Human CD3/CD28 T Cell Activator (StemCell) and 20 ng/mL Recombinant Human IL-2 (rhIL-2; Biolegend), seeded in a F-bottom 96-well plate using 4×105 cells (200 µl ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 300 g for 5 min at 4°C and cultured in flasks at 106 cells/mL in complete RMPI with 20 ng/mL recombinant mouse IL-2 (rmIL-2; Biolegend). To generate talin-deficient T cells ...
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bioRxiv - Immunology 2024Quote: ... splenocytes (2*107/mL in IMDM medium containing 2% FCS) were simultaneously stained with anti-B220-Alexa647 (clone RA3-6B2 Biolegend) and loaded with 5 μM Indo-1 acetoxymethyl (Indo1-AM ...
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions were resuspended in FACS buffer (PBS, 2%FBS, 0.01%Tween) and stained for viability dye (1/1000, Biolegend) in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4 °C with the following antibodies: CD271-PE (1:200; ME20.4, BioLegend), CD235a-PECy7 (1:20 ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse CD63-160Gd (clone NVG-2, BioLegend), anti-mouse CD120b-166Er (clone TR75-32.4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse IL-2 (clone JES6-1A12, BioLegend) antibody was used to capture IL-2 from the supernatants ...
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bioRxiv - Immunology 2020Quote: ... A647 anti-Bcl-2 (633510) (all from BioLegend); APC anti-CXCR4 (558644) ...
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bioRxiv - Immunology 2022Quote: ... anti-CD138 (BV711, 281-2, AB_2562571, BioLegend #142519), anti-CD19 (BV785 ...
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bioRxiv - Bioengineering 2021Quote: ... IL-2 PE-Cy7 (Biolegend clone MQ1-17H12), IFNγ APC (Biolegend clone B27) ...
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bioRxiv - Immunology 2020Quote: ... CD49d (α4-integrin; FITC, Biolegend, Clone: R1-2), CD88 (PE ...
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bioRxiv - Immunology 2022Quote: ... and 10 ng/mL IL-2 (589102, BioLegend) in RPMI-1640 complete medium for 24 hours before killing to differentiate into T cells ...
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bioRxiv - Immunology 2022Quote: ... BV785-labeled anti-CD138 (clone: 281-2; BioLegend), APC-labeled anti-IgD (clone ...
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bioRxiv - Immunology 2022Quote: ... APC-labeled anti-IgD (clone: IA6-2; BioLegend), PerCP-Cyanine5.5-labeled anti-CD38 (clone ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD32-PE-Cy7 (FUN-2, Biolegend). After ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of Trustain FcR blocking reagent (BioLegend), 3 μl of anti-human CD45 antibody (clone HI100 ...
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bioRxiv - Bioengineering 2020Quote: ... Then a biotinylated anti-IL-2 Ab (BioLegend) at a concentration of 0.25 μg/ml was added and after washing ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated SARS-CoV-2-S1 Protein (Biolegend 793804) Anti-Biotin (409003) ...
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bioRxiv - Immunology 2020Quote: ... and rIL-2 (10 U/ml; Biolegend, #575402), with or without 2.5 μM mCRAMP ...