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Citations for BioLegend :
3401 - 3450 of 4492 citations for 2 4 6 Trichlorophenol 13C6 99% 100 Ug Ml In Isooctane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Two different tissue sections were immunostained using conjugated antibodies as follows: 1) F4/80-FITC (1:100; BioLegend; 123107), CD11b-PE (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed one time with FACS buffer and then resuspended in 100 μL of an antibody cocktail containing the following: murine anti-CD45i.v.+ (Biolegend, cat#103112 ...
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bioRxiv - Neuroscience 2023Quote: ... cat no: 130-123-284) and allophycocyanin (APC) conjugated rat anti-mouse CD45 (1:100, Biolegend, cat no: 103111) in blocking solution for 30 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45highCD8+ cells were labeled with rat anti-CD45 APC (1:100, catalog no. 103111; BioLegend), rat anti-CD8 PerCP/Cyanine5.5 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were stained at the cell surface with FITC- conjugated anti-CD4 (1:100, BioLegend Cat# 203406 (also 203405), RRID:AB_1227553 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated with FITC, and PE-conjugated antibodies (1:100, IFNr or FoxP3, GZMB) purchased from Biolegend. Aqua LIVE/DEAD used for cell viability was purchased from Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... cell pellet was resuspended in 100 µl ice cold PBS and 0.2 µL Zombie NIR viability dye (BioLegend, 77184). Cells were kept on ice until their flow cytometry analysis using FACS Canto II from BD-Biosciences.
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bioRxiv - Genomics 2023Quote: ... Cells were stained on ice in the dark for 30 mins with CD34-APC (clone 581; Biolegend; 1:100), CD38-PE/Cy7 (clone HB7 ...
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bioRxiv - Immunology 2023Quote: ... Primary and conjugated antibodies used in this study were: Armenian hamster anti-CD11c pacific blue (Biolegend, 1:100, #117321), goat anti-HIF-1α (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... 1:300), NK1.1 (108736, 1:150), Tbet (644810, 1:50), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, CD19 (45-0193-82 ...
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bioRxiv - Immunology 2023Quote: ... Roughly 0.5 x 106 cells were washed in FACS buffer (PBS + 3% FCS + 0.1% sodium azide) and stained with either FITC-coupled anti-FcεR1 (1:100, clone MAR-1, BioLegend, #134306) and PE-coupled anti-CD117 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 45 min on ice in the dark with an APC anti-IFNγ (1:100 dilution, clone XMG1.2, Biolegend) intracellular marker ...
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bioRxiv - Cell Biology 2023Quote: ... Osaka University) were pre-incubated with anti-human-Fc secondary antibodies fused to APC (BioLegend, #410712, dilution 1:100) for 1 h in 1% BSA in PBS ...
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bioRxiv - Immunology 2023Quote: ... and around 1×106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, dilution 1/100), Sca-1 (APC-Cy7 ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were incubated with a primary rabbit anti-MMP2 antibody (cat# 87809, BioLegend, San Diego, CA; 1:100) overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Cells in each well were centrifuged at 350 g for 5 minutes and resuspended in 100 μL of PBS premixed with 0.5 μL of Trustain FcX plus blocking solution (BioLegend) on ice for 10 min ...
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bioRxiv - Cell Biology 2024Quote: Antibodies used in this study were from the following sources: ALIX (634502, IF 1:100) was purchased from BioLegend. IST1 (51002-1-AP ...
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bioRxiv - Cancer Biology 2024Quote: ... washed and re-suspended in the following primary antibodies: a rat monoclonal (IgG2a) anti-mouse FRβ (BioLegend,1:100) or a sheep polyclonal anti-mouse FRα (R&D Systems ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended in cell staining buffer (PBS + 5 mM EDTA + 1% BSA + 1.6 mM NaOH + 0.01% sodium azide) and incubated in 1:100 diluted Zombie UV viability dye (Biolegend, 423107) or Zombie Violet viability dye (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-436 cells were resuspended in cell staining buffer (PBS + 5 mM EDTA + 1% BSA + 1.6 mM NaOH + 0.01% sodium azide) and incubated in 1:100 diluted Zombie Violet viability dye (Biolegend, 423113) for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were first incubated with True Stain fcXTM antibody (anti-mouse CD16/32, BioLegend, #101319, clone 93, 1:100) in PBS containing 0.5% BSA and 2 mM EDTA before staining with antibodies directly conjugated to fluorophores for flow cytometry ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were stained in 100 µL 1X PBS containing 0.5% (v/v) Zombie UV fixable viability dye (BioLegend # 423108) on ice for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then resuspended in 50uL of PBS containing fluorophore-conjugated antibodies for CD11b (1:100, Biolegend, 101216), MHCII (1:100 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated for 20 min at room temperature in 100 μl of FACS buffer (0.1% FBS in PBS) containing fluorochrome-labeled antibodies (BioLegend) against human CD3 (Pacific blue ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C – chicken anti-keratin 14 (1:800, Biolegend 906001), rabbit anti-SCD1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell surface staining was done for 30 minutes at 4 degrees with the following antibodies (all from BioLegend, San Diego, CA): CD45 (30-F11) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS with a fluorochrome-conjugated antibody (mouse monoclonal APC-conjugated anti-CD69 (BioLegend 10909); mouse monoclonal PE-conjugated anti-CD25 (Pharmingen 30795X ...
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bioRxiv - Bioengineering 2020Quote: ... anti-Pax7 (mouse monoclonal, DHSB, diluted 1:4) and anti-Neurofilament H phospho (rabbit polyclonal, BioLegend # PRB-573C, diluted 1:400). As secondary antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Pathology 2022Quote: ... Cells were washed (PBS; twice), centrifuged (300g; 5min.; 4°C) and stained (10min., RT) with Streptavidin (Alexa Fluor® 647-conjugated, Biolegend, #405237 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Neuroscience 2022Quote: ... The homogenates were first blocked in PBS with 10% rat serum with gentle rotation in a cold room and then stained for 1h protected from light at 4°C with CD206-FITC (cat no. 141704, Biolegend®) + MHCII-PE (cat no ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with the following antibodies in isolation or in combination for 30-60 minutes in the dark at 4°C: ICAM-1 PE (BioLegend, #353106), CCR6 PE/Dazzle (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend, 103149) CD11b-PE (BioLegend ...
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... cells were washed with PBS twice before staining with the following antibodies or reagents for another 30 min at 4°C in the dark: Streptavidin-BV650 (Biolegend, UK), IgG (H+L ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...