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3401 - 3450 of 10000+ citations since 2019

• Myeloid progenitor dysregulation fuels immunosuppressive macrophages in tumors

  Samarth Hegde, et al., bioRxiv - Immunology 2024

  Quote: ... and CD16/CD32 (clone 93, Biolegend) for 15 mins on ice prior to surface staining ...
• Antigen specificity shapes antibody functions in tuberculosis

  Joshua R. Miles, et al., bioRxiv - Immunology 2024

  Quote: ... followed by CD16a.NK-92 cells (5 × 104 cells/well) with brefeldin A (Biolegend), Golgi Stop (BD Biosciences ...
• Antigen specificity shapes antibody functions in tuberculosis

  Joshua R. Miles, et al., bioRxiv - Immunology 2024

  Quote: ... was performed in permeabilization buffer (Biolegend). Markers were measured using a BD LSR Fortessa and analyzed by FlowJo as described above.
• Antigen specificity shapes antibody functions in tuberculosis

  Joshua R. Miles, et al., bioRxiv - Immunology 2024

  Quote: ... and anti-CD107a (H4A3, Biolegend) (5 hours ...
• B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

  Naeun Lee, et al., bioRxiv - Immunology 2024

  Quote: ... PE/Cy5-CD11c (BioLegend, #117316), and PE-CSF1R (Invitrogen ...
• B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

  Naeun Lee, et al., bioRxiv - Immunology 2024

  Quote: ... Brilliant Violet 510-CD11b (BioLegend, #101263), PE/Cy5-CD11c (BioLegend ...
• B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

  Naeun Lee, et al., bioRxiv - Immunology 2024

  Quote: ... APC/Cy7-CD21 (BioLegend, #123418), PE/Cy7-CD23 (eBioscience ...
• B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

  Naeun Lee, et al., bioRxiv - Immunology 2024

  Quote: ... Brilliant Violet 421-IgD (BioLegend, #348226), and PE-MPO (Invitrogen ...
• B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

  Naeun Lee, et al., bioRxiv - Immunology 2024

  Quote: ... PE-CD95 (BioLegend, #152608), APC-CD138 (Invitrogen ...
• Autoimmunity and clinical pathology amelioration in SLE by Dexamethasone primed Mesenchymal Stem Cell derived conditioned media

  Khushbu Priya, et al., bioRxiv - Immunology 2024

  Quote: ... and CD1d (PE; BioLegend) antibodies were used to stain for surface antigens for 30 min at 4°C in dark ...
• Autoimmunity and clinical pathology amelioration in SLE by Dexamethasone primed Mesenchymal Stem Cell derived conditioned media

  Khushbu Priya, et al., bioRxiv - Immunology 2024

  Quote: ... CD25 (APC; BioLegend) and for Bregs immunophenotyping ...
• Autoimmunity and clinical pathology amelioration in SLE by Dexamethasone primed Mesenchymal Stem Cell derived conditioned media

  Khushbu Priya, et al., bioRxiv - Immunology 2024

  Quote: ... CD5 (PerCP; BioLegend) and CD1d (PE ...
• Ancestral SARS-CoV-2 immune imprinting persists on RBD but not NTD after sequential Omicron infections

  Zuowei Wang, et al., bioRxiv - Immunology 2024

  Quote: ... CD19-PE/Cy7 (BioLegend, 302215), CD27-APC (BioLegend ...
• Ancestral SARS-CoV-2 immune imprinting persists on RBD but not NTD after sequential Omicron infections

  Zuowei Wang, et al., bioRxiv - Immunology 2024

  Quote: ... they were stained with cell-surface antibodies: CD3-BV510 (BioLegend, 317331), CD19-PE/Cy7 (BioLegend ...
• Ancestral SARS-CoV-2 immune imprinting persists on RBD but not NTD after sequential Omicron infections

  Zuowei Wang, et al., bioRxiv - Immunology 2024

  Quote: ... Streptavidin-APC (BioLegend, 405207) and DAPI were added to the wells at 4°C for 30min ...
• Glioma-associated tertiary lymphoid structures are sites of lymphocyte clonal expansion and plasma cell formation

  Pinar Cakmak, et al., bioRxiv - Immunology 2024

  Quote: ... CD138 (channel 594, 352324, BioLegend). For nuclei detection ...
• Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

  KayLee K. Steiner, et al., bioRxiv - Immunology 2024

  Quote: ... Naïve T cells were skewed to the following subsets with cytokines: Th1 (10ng/mL mIL-12 (Biolegend, 577004), 10ng/mL recombinant human IL-2 ...
• Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

  KayLee K. Steiner, et al., bioRxiv - Immunology 2024

  Quote: ... CD8a APC (1:200 BioLegend, 100712), CD62L PE (1 ...
• Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

  KayLee K. Steiner, et al., bioRxiv - Immunology 2024

  Quote: ... CD71 PE-Cy7 (1:200 BioLegend, 113812), CD25 PE-Cy5 (1:100 Invitrogen ...
• Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

  KayLee K. Steiner, et al., bioRxiv - Immunology 2024

  Quote: ... CD62L PE (1: 200 BioLegend, 104408), CD62L APC (1:200 BioLegend ...
• Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

  KayLee K. Steiner, et al., bioRxiv - Immunology 2024

  Quote: ... CD62L APC (1:200 BioLegend, 104412), CD44 PE-Cy7 (1:300 BioLegend ...
• Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

  KayLee K. Steiner, et al., bioRxiv - Immunology 2024

  Quote: ... CD44 PE-Cy7 (1:300 BioLegend, 103030), PD1 PE (1 ...
• Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

  KayLee K. Steiner, et al., bioRxiv - Immunology 2024

  Quote: ... CD45 FITC (1:200 BioLegend, 157214), CD127 PECy7 (1:50 BioLegend ...
• Disentangling cell-intrinsic and extrinsic factors underlying gene expression evolution

  Alexander L. Starr, et al., bioRxiv - Evolutionary Biology 2024

  Quote: ... PE-Cy7-anti rat CD45 (Biolegend: 202214). Donor chimerism was analyzed by detecting CD45 negative ...
• High-resolution atlas of the developing human heart and the great vessels

  Semih Bayraktar, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
• Endothelial deletion of p53 generates transitional endothelial cells and improves lung development during neonatal hyperoxia

  Lisandra Vila Ellis, et al., bioRxiv - Developmental Biology 2024

  Quote: ... PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), Alexa Fluor 488 rat anti-CD324 (ECAD ...
• PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity

  Koki Nakamura, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-HA-tag (BioLegend, 901501; 1:2,000), anti-FKBP8 (R and D systems ...
• Macrophage subtypes inhibit breast cancer proliferation in culture

  Sophia R.S. Varady, et al., bioRxiv - Cell Biology 2024

  Quote: ... cells were suspended in BV711-CD11B antibody (macrophage marker; BioLegend, 301344, 1:20 dilution) for 30 minutes ...
• Macrophage subtypes inhibit breast cancer proliferation in culture

  Sophia R.S. Varady, et al., bioRxiv - Cell Biology 2024

  Quote: ... conjugated to PE-Cyanine7 (305218, BioLegend, 1:20 dilution) and a marker for M2 macrophages ...
• Macrophage subtypes inhibit breast cancer proliferation in culture

  Sophia R.S. Varady, et al., bioRxiv - Cell Biology 2024

  Quote: ... and pellets were resuspended in BV711-CD11B antibody (macrophage marker; BioLegend, 301344) suspended in flow buffer (1:20 dilution ...
• Macrophage subtypes inhibit breast cancer proliferation in culture

  Sophia R.S. Varady, et al., bioRxiv - Cell Biology 2024

  Quote: ... conjugated to AlexaFluor 488 (321113, BioLegend, 1:20 dilution) were used to assess polarization status of macrophages at both 24 ...
• The Rubicon-WIPI axis regulates exosome biogenesis during aging

  Kyosuke Yanagawa, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-ALIX (BioLegend, 634502, 1:1000), anti-Calnexin (Sigma-Aldrich ...
• The Rubicon-WIPI axis regulates exosome biogenesis during aging

  Kyosuke Yanagawa, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-ALIX (BioLegend, 634502, 1:200), goat anti–mouse IgG H&L Alexa Fluor 488 pre-adsorbed (Abcam ...
• Oligosaccharyltransferase is involved in targeting to ER-associated degradation

  Marina Shenkman, et al., bioRxiv - Cell Biology 2024

  Quote: ... Mouse anti-HA was from Biolegend and anti-Myc was custom produced and used before (35) ...
• Divergent roles of SOX2 in human and mouse germ cell specification related to X-linked gene dosage effects

  Wenteng He, et al., bioRxiv - Cell Biology 2024

  Quote: ... and CD49f (INTEGRINα6, Biolegend, 313624) as previously reported.24 Briefly ...
• Inter-cellular mRNA Transfer Alters Human Pluripotent Stem Cell State

  Yosuke Yoneyama, et al., bioRxiv - Cell Biology 2024

  Quote: ... were mixed in Cell Staining Buffer (BioLegend), and applied to 100 μl of 2– 5×105 of single cells per reaction ...
• Intestine-on-chip enhances nutrient and drug metabolism and maturation of iPSC-derived intestinal epithelial cells relative to organoids and Transwells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... The remainder of the cells were stained using the Zombie Aqua Fixable Viability Kit (BioLegend #423101) according to the manufacturer’s instructions ...
• Constant surface area-to-volume ratio during cell growth as a design principle in mammalian cells

  Weida Wu, et al., bioRxiv - Cell Biology 2024

  Quote: ... APC anti-mouse CD54 Antibody (BioLegend, RRID:AB_10612936, Cat#116120) and PE anti-mouse CD98 (4F2 ...
• HIV-1 controllers possess a unique CD8+ T-cell activation phenotype and loss of control is associated with increased expression of exhaustion markers

  Amber D Jones, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were also stained for viability using the live/dead stain Zombie Yellow (BioLegend). Cell enumeration was carried out using Cytek FACSort DxP12 flow cytometer and data analysis using FlowJo v10.6.1 software.
• HIV-1 controllers possess a unique CD8+ T-cell activation phenotype and loss of control is associated with increased expression of exhaustion markers

  Amber D Jones, et al., bioRxiv - Immunology 2024

  Quote: ... and/or anti-Tim-3 (CD366) antibody (BioLegend, UltraLEAF purified anti-human ...
• The role of LEDGF in transcription is exploited by HIV-1 to position integration

  Rakesh Pathak, et al., bioRxiv - Genomics 2024

  Quote: ... RNA Pol II (8WG16 BioLegend; Cat# 664912), H3K4me3 (C42D8 Rabbit mAb #9751 cell signaling) ...
• Single-cell analysis of ovarian myeloid cells identifies aging associated changes in macrophages and signaling dynamics

  Zijing Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... and PerCP/Cy5.5-CD11b (BioLegend® 101228) in 1x PBS with 5% FBS for 20 minutes ...
• Single-cell analysis of ovarian myeloid cells identifies aging associated changes in macrophages and signaling dynamics

  Zijing Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... F4/80 (BioLegend® 123149), CD68 (BioLegend® 137023) ...
• Single-cell analysis of ovarian myeloid cells identifies aging associated changes in macrophages and signaling dynamics

  Zijing Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... The cells were then pooled and stained with fluorochrome-conjugated primary antibodies BV650-CD45.2 (BioLegend® 109835) and PerCP/Cy5.5-CD11b (BioLegend® 101228 ...
• Single-cell analysis of ovarian myeloid cells identifies aging associated changes in macrophages and signaling dynamics

  Zijing Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... CD11b (BioLegend® 101267,), F4/80 (BioLegend® 123149) ...
• The Impact of Parabacteroides distasonis Colonization on Hosts’ Microbiome, Metabolome, Immune Responses, and Diabetes Onset

  Khyati Girdhar, et al., bioRxiv - Immunology 2024

  Quote: ... The supernatant was used to determine the IL-2 secretion using the IL2 Elisa kit (#BioLegend), and the cells were used to determine the protein concentration using BCA assay (#Thermo ...
• Phospholipase Cγ regulates lacrimal gland branching by competing with PI3K in phosphoinositide metabolism

  Qian Wang, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Pax6 (#RPB-278P) from Biolegend. Immunostaining of phospho-ERK1/2 ...
• Epigenetic changes regulating the epithelial-mesenchymal transition in human trophoblast differentiation

  William E. Ackerman IV, et al., bioRxiv - Developmental Biology 2024

  Quote: ... or with an anti-integrin ß4 antibody coupled to R-phycoerythrin (ITGß4-PE, 1:500, clone 58XB4, BioLegend, San Diego CA) for isolation of CTB ...
• Human myogenic progenitor cells display tenogenic differentiation potential and facilitate tendon regeneration

  Xiexiang Shao, et al., bioRxiv - Cell Biology 2024

  Quote: ... and PE anti-human CD56 (BioLegend, cat#304606) for 45 min at 4°C ...
• Downregulation of PIK3IP1/TrIP on T cells is controlled by TCR signal strength, PKC, and metalloprotease-mediated cleavage

  Benjamin M. Murter, et al., bioRxiv - Cell Biology 2024

  Quote: ... (clone 37.51; Biolegend: 102101).