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Citations for BioLegend :
3351 - 3400 of 10000+ citations for Anti CD25 SAP human Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Stimulated PBMCs were maintained in RPMI-1640 supplemented media with 100 ng/ml CD3 and 10 ng/ml recombinant human IL-2 (Biolegend, 589104).
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected on day 6 and immediately assessed for cytokine and chemokine secretion by LEGENDplex™ using LEGENDplex Human Inflammation Panel 1 (13-plex) with V-bottom Plate (Biolegend catalog #740809 ...
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bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... cell pellets resuspended in 100 μl FACS buffer (phosphate buffer saline containing 1% BSA and 0.1% sodium azide) were treated with human TruStain FcX™ (Biolegend #422302) for 15 min on ice followed by incubation with rabbit anti-human LBR antibody (Abcam #32535 ...
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bioRxiv - Immunology 2021Quote: ... using the LegendPlex kit (BioLegend).
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bioRxiv - Immunology 2021Quote: ... using the LegendPlex kit (BioLegend).
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bioRxiv - Neuroscience 2021Quote: ... IL-1β ELISA kit (Biolegend) was used for determination in plasma and ELISA was performed according to manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... c-kit-BV605 (104D2; BioLegend), CRTH2-PE (MACS Milltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... or respective ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... permeabilized (BioLegend cytokine staining kit) and stained with anti-cytokine antibodies.
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bioRxiv - Developmental Biology 2021Quote: ... anti-EpCAM-APC (eBioscience, G8.8) and anti-CD24-Pacific Blue (Biolegend, M1/69). Cells were resuspended with SMEM media (Sigma) ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD107b (Mac-3) and anti-mouse TNF (MP6-XT22) (all Biolegend). Live-Dead Fixable Green and Live-Dead Fixable Aqua Dead Cell stains was purchased from Thermofisher (Boston ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used were mouse anti-HA (1:1000, Anti-HA.11, Biolegend), rat anti-somatostatin (1:100 ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... Biotinylated secondary anti-rabbit and anti-mouse antibodies (Biolegend Inc. #406401 and #405303) were applied for 1 h and then incubated with ABC (Vectastain ...
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bioRxiv - Genomics 2019Quote: ... using anti Ter119 (PE-Cy7 labelled) and anti CD117 (PE labelled) antibodies (Biolegend). 0h of erythropoiesis was defined as (CD117+,Ter119- ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with anti-CD4 (GK1.5) and anti-CD8 (53-6.7) (Biolegend). For detection of intracellular cytokines ...
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bioRxiv - Molecular Biology 2019Quote: ... mesoderm progenitors were isolated by FACS with anti-CD326 and anti-CD56 (Biolegend). CPC population was obtained by formation of embryoid bodies with H9 cells using BMP4 ...
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bioRxiv - Immunology 2021Quote: ... anti-GL7 Alexa Fluor 488 and anti-CD95 PE (Biolegend. San Diego, CA). For Ag-specific detection ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with anti-IFN-g or anti-IL-17A antibodies (Biolegend); For intracellular FoxP3 staining ...
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bioRxiv - Immunology 2021Quote: ... anti-TIGIT-PerCPCy5.5 (clone A15153G) and anti-HLA-DR (clone L243) (from BioLegend). PBMCs were washed with PBS and fixed and permeabilized with BD Cytofix/CytoPerm following manufacturer’s protocol (Cat ...
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bioRxiv - Immunology 2020Quote: ... or anti-CD45-PerCP (2D1) and anti-CD19-AF488 (HIB19) (all from BioLegend). After 15 minutes of incubation at RT ...
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bioRxiv - Cell Biology 2021Quote: ... anti-mouse CD3 (145-2C11) and anti-mouse CD28 (37.51) were from BioLegend. Anti-human CD3 (OKT3 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-CD44 PE conjugated (Pharmingen-553134) and anti-CD62L FITC conjugated (Biolegend-104406).
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bioRxiv - Immunology 2021Quote: ... rat anti-mouse IL-6 or rat anti-mouse Ly6G (all from Biolegend), followed by fluorescence-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... cells were restimulated on plates coated with anti-CD3 and anti-CD28 (BioLegend). After 48 hours of restimulation ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620) and anti-Ki67 (Biolegend, clone 16A8), were diluted 1:200 for staining ...
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bioRxiv - Immunology 2024Quote: ... Samples were incubated with anti-CD16/anti-CD32 blocking antibodies (Biolegend, Cat. 101302) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... before incubation with mouse anti-HA antibody (anti-HA.11 clone 16B12, BioLegend) in 0.01% Triton X-100 in PBS for 45 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-pan-cytokeratin-AF532 antibody (Nanostring) and anti-CD45-AF594 antibody (BioLegend, 103144) were used ...
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bioRxiv - Immunology 2023Quote: ... anti-podoplanin (8.1.1) and anti-TCR-beta chain (H57-597) were from BioLegend. Anti-CD45 (30-F11 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Toll-like receptor 2 (anti-TLR2; Clone T2.5 for flow cytometry, Biolegend 121810 ...
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bioRxiv - Bioengineering 2024Quote: ... FITC anti-mouse CD8a antibody and APC/Cyanine7 anti-mouse CD4 antibody (BioLegend) in PBS ...
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bioRxiv - Immunology 2024Quote: ... purified anti-CD3ε (145–2C11) and anti-CD28 (37.51) antibodies obtained from BioLegend were employed at the appropriate concentrations as per the experiment design ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat: 324420; anti-EpCAM-AF647, Biolegend, Cat: 118211) at 4 ºC for >30 min ...
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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bioRxiv - Immunology 2023Quote: ... CD4 (RM4-5), (anti-CD90.2 [30-H12], anti-CD44 [IM7], and anti-CD27 [LG-7F9]) (all from eBioscience/Invitrogen and Biolegend, San Diego, CA). Phenotype of polyclonal CD4+ T cell subsets and Plasmodium B5-specific CD4+ T cell subsets were identified with gating strategy as schematically depicted in S7 Fig ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... the supernatants were collected to determine the levels of antibody productions (e.g., IgG1, IgG2, IgG3, IgG4, IgA, and IgM) by using LEGENDplex™ Human Immunoglobulin Isotyping Panel (Biolegend, CA, USA). The Gallios Flow Cytometer was utilized to analyze the data according to the manufacturer’s recommended protocol.
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bioRxiv - Genomics 2020Quote: ... The cells were centrifuged at 300xg for 5 minutes at room temperature and resuspended in 85 ul of staining buffer and incubated with 5 ul of Human TruStain FcX(Biolegend cat: 422301) for 10 minutes on ice in 5 ml FACS tubes ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... HLA-A2 positive PBMC samples were further stained with PE labelled tetramer (home-made) and APC labelled human CD8+ antibody (BioLegend Cat#344721), and acquired with flow cytometer FACS Canto (BD).
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...