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Citations for BioLegend :
3301 - 3350 of 7869 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... BD Biosciences (human CD45-PE, clone HI30, 1:200, mouse CD45-APC-Cy7, clone 30-F11, 1:100, BioLegend). Upon confirmation of engraftment ...
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bioRxiv - Genomics 2024Quote: ... DMEM containing 0.2% BSA and 1mg/ml DNase stained with 1:10,000 DAPI and 1:1000 Draq5 (BioLegend, 424101), and live cells were sorted ...
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bioRxiv - Bioengineering 2024Quote: Cells were washed with PBS and incubated in 5 uL of Human TruStain FcX™ (Fc Receptor Blocking Solution) (Cat. No. 422301, Biolegend, San Diego, California, United States), in 100uL FACS buffer (2% FBS in 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were permeabilized and blocked with 0.1% Triton X-100 in PBS supplemented with 10% normal goat serum for 1 hour at room temperature and incubated with mouse anti-Tuj1 (1:300; BioLegend) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... A cocktail of Abs for surface markers at the predetermined concentrations (CD31-APC 1:100, Biolegend; CD45-APC 1:200, Biolegend, CD117-APC 1:100 ...
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bioRxiv - Immunology 2021Quote: ... at 2:1 ratio for 1 h at 4C to ensure maximum staining quality before surface staining with CD20-FITC (2H7, Biolegend) for an additional 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... DC purity was assessed by antibodies against CD141 (1:10, APC, AD5-14H12, 130-113-314, Miltenyi) and XCR1 (1:10, PE, S15046E, 372604, BioLegend), and T cell phenotype was assessed by antibodies against CD25 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: Free floating 30 μm sagittal brain sections were treated for antigen retrieval using sodium citrate buffer and subsequently stained using primary antibodies for Iba1 (1:250, rabbit, Wako) and anti-6E10 (1:1000, Biolegend), and the corresponding secondary antibodies and nuclear counterstain using DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... All FACS antibodies were used at 1:100 dilution in the presence of TruStain fcX blocker (101320, Biolegend, 1:50). For PDGFRa immunofluorescence staining ...
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bioRxiv - Cancer Biology 2020Quote: ... and 200 μl of primary antibody (1:200 anti-αSMA antibody, eBioscience, clone 1A4, efluor 570; 1:200 anti-CD31 antibody, Biolegend, clone MEC13.3 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% Sodium Azide and with one of the following primary antibody dilutions: Mouse Anti-HA (1:2000; HA.11, Biolegend), Mouse Anti-V5 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Stained cells and “fluorescence minus one” controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Biolegend, #424001) as a live/dead stain ...
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bioRxiv - Immunology 2022Quote: ... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U/ml erythropoietin) for 1 hour following disaggregation before being stained (anti-CD71 Brilliant Violet 421, 1:5000 dilution – 113813 Biolegend, RRID ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with K562 at a 1:1 E/T ratio with addition of a CD107a FITC mAb (H4A3, BioLegend) in a 96-round bottom plate with a centrifugation step of 5min at 500rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of protein (1/10 of input transferred in a new tube) was incubated together with 1 μL of anti-HA antibodies (BioLegend 901502 anti-HA.11 clone 16B12 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5mM EDTA and 1% BSA) and incubated with the following primary antibodies: anti-mouse CD45 (Brilliant Violet 570, 30-F11, 1:200, Biolegend), anti-mouse Fce-R1 alpha (PE-Cyanine7 ...
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bioRxiv - Immunology 2020Quote: ... and other epithelial cells markers (anti-mouse CD326 Brilliant Violet 605, 1:200 Biolegend 118227 and PerCP/Cy5.5 anti-mouse CD24, 1:200 Biolegend 101824) for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... for sorting or CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-pacific blue (Biolegend, clone GoH3, 1:200) for analysis ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with the tetramer plus CD3-eFluor 450 (1/100, 48-0032-82 eBioscience) and CD8-FITC (1/100, 300905 BioLegend) at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Heparinized blood diluted 1:1 in PBS overlaid onto Lymphopure density gradient medium 1.077 g/mL (BioLegend, San Diego, USA) in Leucosep tubes (Greiner Bio-One ...
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bioRxiv - Immunology 2021Quote: ... splenocytes from immunized mice were stained with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin followed by 1 μg/mL of streptavidin (BioLegend) at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Candidate TCR-transduced T cells were then added in at an E:T ratio of 1:1 for 6h in the presence of anti-CD107A-BV650 (Clone H4A3, Biolegend 328638) and Golgi Block ...
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bioRxiv - Immunology 2021Quote: ... isotype controls and inhibitor of VLA-4 were used at 1 μg/ml: LEAF™ purified anti-CD29 (clone HMβ1-1, 102209, BioLegend), LEAF™ purified anti-CD31 (clone MEC13.3 ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with anti-CD3-FITC (clone 145-2C11; 1:100; eBioscience) and anti-TCRδ (clone GL3; 1:100; Biolegend) for 30 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1×PBS) mixed with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:200, Clone: 93, BioLegend, CA, USA) and eBioscience™ Fixable Viability Dye eFluor™ 780 (1:1000 ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... Fluorescently conjugated integrin antibodies (1:400 AF488 anti-integrin β1, clone-TS2/16, BioLegend; 1:200 PE anti-integrin β1, clone-TS2/16, BioLegend; 1:400 AF647 anti-integrin β1 ...
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bioRxiv - Neuroscience 2022Quote: ... according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies: mouse anti-CD9 (1:1000; Biolegend), mouse anti-CD63 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... using 1:1000 of polyclonal rabbit anti-human MCT1 IgG (Cell Signalling) and 1:1000 mouse anti-human CD147 IgG (Biolegend) followed by HRP-conjugated secondary IgG antibody of specific host type ...
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... were used to confirm results with McSA1: a mouse monoclonal antibody to Aβ residues 1-16 (1:1000; clone 6E10; Cat#803001, Biolegend) or a mouse monoclonal antibody to Aβ residues 17-24 (1:1000 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and then incubated with the primary antibodies α-FLAG polyclonal mouse (1:12,000) and α-RpoA monoclonal mouse (1:1,000) (Biolegend). Then ...
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bioRxiv - Physiology 2023Quote: ... Samples were next stained with surface marker antibodies (CD45-AF700 1:500 103128 Biolegend, CD31-Pacific Blue 1:500 102421 Biolegend, TER119-BUV496 1:500 741079 BD ...
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bioRxiv - Immunology 2023Quote: ... Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST, BioLegend).
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bioRxiv - Microbiology 2023Quote: ... cells were washed in cold PBS/1% (w/v) BSA and stained for 1 hour at 4°C with AlexaFluor647-conjugated anti-BST2 (Biolegend) or control isotype (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... Stained T-IEL and unstained lymph node T cells were mixed at a 1:1 ratio before stimulation with 30 μg/ml anti-CD3 (Clone 145-2C11, Biolegend) crosslinked with 5 μg/ml anti-Hamster (Jackson Immunoresearch) ...
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bioRxiv - Immunology 2023Quote: ... Each sample was labelled with one specific hashtag (1:400 dilution) and CITE-seq antibodies (1:800 dilution, all Biolegend). After labelling for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... for 5 min on ice before staining with fluorochrome-conjugated antibodies in FACS staining buffer (1× PBS, 2% FBS): CD3 (1:50 dilution; clone 145-2C11, BioLegend), CD8α (1:200 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA and actin were stained with 1 μg/mL DAPI and phalloidin (1:1000, Flash Phalloidin™ Red 594; BioLegend). Testis were washed three times with PBS and mounted in (ProLong™ Gold Antifade Mountant ...
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bioRxiv - Immunology 2023Quote: ... CXCR5-PE-Dazzle 594 (J252D4, 1:40), and CD38-PE-Cy7 (HIT2, 1:200) for GC B cells (all BioLegend). For donors 321-07 and 321-08 the PBMC samples for days 0 and 5 were stained with TotalSeq-C anti-human hashtag oligos 9 and 10 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30 min at 25 °C with BCL6-PE (7D1, 1:50) and Ki-67-PE-Cy7 (Ki-67, 1:400) (both BioLegend). Cells were washed twice with permeabilization/wash buffer and resuspended in P2 for acquisition on an Aurora using SpectroFlo v2.2 (Cytek) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with FACs buffer (1× PBS, 1% FBS) and fixed at RT for 20 min with Intracellular Staining Fixation Buffer (BioLegend). Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS ...