6 BROMO 4 4 DIMETHYL 1H BENZO D 1 3 OXAZINE 2 4H THIONE citations for BioLegend

Quote: ... cells were re-stimulated with plate-bound CD3 and soluble CD28 antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days before infection with HIV-1 reporter viruses ...

Nanoscale colocalization of NK cell activating and inhibitory receptors controls signal integration

David Tomaz, et al., bioRxiv - Immunology 2022

Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...

MAIT cells are minimally responsive to Mycobacterium tuberculosis within granulomas, but are functionally impaired by SIV in a macaque model of SIV and Mtb co-infection

Amy L. Ellis-Connell, et al., bioRxiv - Immunology 2020

Quote: ... Cells were then incubated for 16 hours at 37°C in the presence of 2 uM monensin (Biolegend; San Diego, CA), 5 μg/mL Brefeldin A (Biolegend ...

Perfect adaptation of CD8⁺ T cell responses to constant antigen input over a wide range of affinity is overcome by costimulation

Nicola Trendel, et al., bioRxiv - Immunology 2019

Quote: ... T cells were pelleted in a V-bottom plate and resuspended in 40 µl PBS with 2% BSA and fluorescent 9V pMHC tetramers that were produced with Streptavidin-PE (Biolegend, 405204) and used at a predetermined dilution (1:100-1:1000) ...

Aging-Associated Decrease in the Histone Acetyltransferase KAT6B Causes Myeloid-Biased Hematopoietic Stem Cell Differentiation

Eraj Shafiq Khokhar, et al., bioRxiv - Cell Biology 2019

Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...

Cytotoxic CD4⁺ T cells driven by T-cell intrinsic IL-18R/MyD88 signaling predominantly infiltrate Trypanosoma cruzi-infected hearts

Carlos-Henrique. D. Barbosa, et al., bioRxiv - Immunology 2021

Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...

BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection

Yunlong Cao, et al., bioRxiv - Immunology 2022

Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...

Interferon regulates stem cell function at all ages by orchestrating mTOR and cell cycle

Damian Carvajal Ibañez, et al., bioRxiv - Neuroscience 2022

Quote: ... 2 mice were used whose cells were labelled with Hashtags oligos (HTO) using the Cell Hashing protocol from Biolegend (TotalSeq-A). All the sorted cells were pooled on the same tube and processed in the Chromium Next GEM Chip G using Chromium Next GEM Single Cell 3’ v3.1.

BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection

Yunlong Cao, et al., bioRxiv - Immunology 2022

Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...

Omicron booster in ancestral strain vaccinated mice augments protective immunities against both the Delta and Omicron variants

Liqiu Jia, et al., bioRxiv - Immunology 2022

Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...

Lymphatic-Preserving Treatment Sequencing with Immune Checkpoint Inhibition Unleashes cDC1-Dependent Antitumor Immunity in HNSCC

Robert Saddawi-Konefka, et al., bioRxiv - Cancer Biology 2022

Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...

Utilizing a PTPN22 gene signature to predict response to targeted therapies in rheumatoid arthritis

Hui-Hsin Chang, et al., bioRxiv - Immunology 2019

Quote: ... PBMCs were then thawed and plated in 24-well plates (2-2.5 millions/1ml/well) pre-coated with anti-CD3 (2.5 ug/ml, HIT3a clone, Biolegend, San Diego, CA) for 24 hours before harvesting ...

Simultaneous, cell-intrinsic downregulation of PD-1 and TIGIT enhances the effector function of CD19-targeting CAR T cells and promotes an early-memory phenotype

Young-Ho Lee, et al., bioRxiv - Immunology 2020

Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...

Cabozantinib unlocks efficient in vivo targeted delivery of neutrophil-loaded nanoparticles into murine prostate tumors

Kiranj Chaudagar, et al., bioRxiv - Cancer Biology 2020

Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...

Intermediary role of lung alveolar type 1 cells in epithelial repair upon Sendai virus infection

Belinda J Hernandez, et al., bioRxiv - Developmental Biology 2021

Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...

A multi-enhancer hub at the Ets1 locus controls T cell differentiation and allergic inflammation through 3D genome topology

Aditi Chandra, et al., bioRxiv - Genomics 2022

Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...

Initial and ongoing tobacco smoking elicits vascular damage and distinct inflammatory response linked to neurodegeneration

Alejandra P. Garza, et al., bioRxiv - Immunology 2022

Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...

Asymmetric cell division safeguards memory CD8 T cell development

Fabienne Gräbnitz, et al., bioRxiv - Immunology 2022

Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...

The ectromelia virus virulence factor C15 facilitates early viral spread by inhibiting NK cell-infected cell contacts

Elise M. Peauroi, et al., bioRxiv - Immunology 2022

Quote: ... Sixteen micron sections were cut and blocked in .01% Triton X 100 with 2% of FBS and donkey serum including TrueStain Monocyte Blocker (BioLegend 426101) for 1h and then stained overnight with the following directly conjugated mAbs ...

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