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Citations for BioLegend :
3201 - 3250 of 6274 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: ... followed by stimulation with plate bound anti-CD3 (2 μg/ml) and anti-ICOS (5 μg/ml; C398.4A; Biolegend) for 24 hours.
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were washed with 1X PBS + 5% FBS + 0.1% sodium azide before being incubated for 20 minutes with Zombie Aqua viability stain (Biolegend) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... PD-1 tetramers were prepared by incubating a 5:1 ratio of biotinylated PD-1 and PE streptavidin (BioLegend) for 10 minutes on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... and kept the remaining wells unstimulated by adding only an equal volume of 2X brefeldin (5 ug/mL, BioLegend). We incubated plates at 37°C for 4 hours.
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each with PBS plus 0.05% Tween-20 and then blocked with 1x Assay Diluent A (BioLegend) for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... dissociated GL261 tumors (5 × 106 cells) were pre-incubated with 1.0 μg of TruStain fcX (anti-mouse CD16/32, clone 93; BioLegend) in 100 μL of FACs Buffer (DPBS with 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were spun down and resuspended in 5 mL of 1x Red Blood Cell Lysis buffer (Biolegend) and incubated on ice for 5 minutes with occasional swirl ...
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bioRxiv - Cell Biology 2022Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Immunology 2022Quote: Thawed 0.5×106 cells washed 3x at 400xg for 5 minutes at room temperature with 1x staining buffer (Biolegend) and kept on ice during the live dead staining using acridine orange and propidium dye to measure the cell viability before proceeding for 10x experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was filtered through a 40 μm filter and then placed in 5 mL RBC lysis buffer (BioLegend) for 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Cancer Biology 2023Quote: ... BV650 anti-CD4 (clone RM4-5, 1:600), AlexaFluor700 anti-CD8 (clone 53-6.7, 1:800) were purchased from BioLegend. e506 Fixable Viability Dye (1:1000) ...
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bioRxiv - Cancer Biology 2023Quote: ... After 72 hours of incubation splenocytes were treated for 5 hours with activation cocktail with brefeldin A1 (Biolegend, 423303) before staining for CD45 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... in duplicate for 2hours at 37°C prior to adding CD16a.NK-92 cells (PTA-6967, ATCC) (5 × 104 cells/well) for 5hours with brefeldin A (Biolegend), Golgi Stop (BDBiosciences ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... an RBD - HC2.RBD.rFC (Metafora – 1:5) was used in conjunction with Alexa 647 donkey anti rabbit Poly4064 (1:50 Biolegend).
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting single cells suspension was used to detect leukocyte populations (CD45 BV421 [clone: 104; Biolegend], CD4 PerCPeFluor710 [RM4-5; eBioscience], CD8a APC-Cy7 [53-6.7; Biolegend], CD19 PE-Cy7 [6D5; Biolegend]) and CD4+ Foxp3+ Treg (CD45 BV421 ...
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bioRxiv - Immunology 2024Quote: Bulk cultured T cells were stimulated with 5 µM peptides for 5hr in the presence of brefeldin A (Biolegend). The cells were collected and stained with specific antibodies against surface markers ...
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bioRxiv - Immunology 2024Quote: ... in presence (stimulated condition) or absence (unstimulated condition) of 5 μg/ml anti-human CD3ε (clone OKT3; #317302, BioLegend) in PBS at 37°C for 2h ...
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bioRxiv - Plant Biology 2020Quote: ... at 1:1000 and DyLight™ 405 goat anti-mouse antibody (BioLegend, CA) at 1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: All fluorescently labeled antibodies used were obtained from commercial sources (eBioscience or Biolegend), including anti-Thy1 ...
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bioRxiv - Cancer Biology 2021Quote: Vcam1 detection: APC anti-mouse CD106 (Vcam1) antibody (BioLegend, San Diego, CA, USA). Cell proliferation ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were additionally stained for 20 min with antibodies against CD45 (Biolegend, 103116) and CD11b (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit antibody for anti non-muscle-myosin-IIB from (BioLegend, Cat. No. 909902) was used at 1:100 (Western blot and immunofluorescence) ...
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bioRxiv - Immunology 2021Quote: Antibodies against HLA-A*02 and HLA-B*07 were purchased from BioLegend and BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... small intestinal epithelial cells were stained with antibodies anti-CD31-PerCP.Cy5.5 (Biolegend, Mec13.3), anti-CD45-PerCP.Cy5.5 ...
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bioRxiv - Developmental Biology 2021Quote: Cells were stained with FITC-conjugated E-cadherin antibody (Biolegend, San Diego, CA). Flow cytometry was performed as described (26 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Antibodies are as follows: APC Mouse anti-human KLRG1 clone SA231A2 (BioLegend #367716), BV421 Mouse anti-human IL7Rα clone HIL-7R-M21 (BD Biosciences #562436) ...
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bioRxiv - Cancer Biology 2022Quote: ... rat anti-mouse antibodies to CD45 (30-F11) and CD11b (M1/70) (Biolegend) were used to exclude murine cells from analysis.
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bioRxiv - Immunology 2022Quote: ... Fcγ receptors were blocked with Human TruStain FcX antibody (BioLegend, San Diego, CA) for 10 min and surface antigens on cells were stained for 40 min at 4 °C ...
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bioRxiv - Immunology 2019Quote: Mice were treated with 0.1mg/mouse of IL-10 blocking antibody (JES52A5, Biolegend) or the isotype control (Rat IgG1 Κ Isotype Control ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies used in western blot are MBP (SMI-99, RRID:AB_2564741, 1:2000, Biolegend), Active β-catenin (#05665 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were applied overnight at 4°C and included SMI31 (Biolegend, 801601), IBA1 (Wako ...
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bioRxiv - Molecular Biology 2020Quote: Primary antibodies used in this study were anti-c-Myc monoclonal 9e10 (Biolegend), anti-HA (Biolegend) ...
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bioRxiv - Molecular Biology 2021Quote: ... The following primary antibodies were used: mouse anti-TUJ1 (Biolegend, 801202, 1:250), rabbit anti-GFAP (Dako ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were incubated with primary antibodies: anti-Rpb3 (Biolegend, 665003 1:1000 dilution), anti-Ser5p (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... and then stained with the following antibodies for 30 minutes: PacBlue-CD31 (Biolegend), PacBlue-CD45 (Biolegend) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then incubated with anti-TUJ1 mouse antibody (Biolegend, 1:1000) in PBS at 4°C for overnight and then further incubated with Alexa Fluor 647-conjugated anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with the following antibodies: CD45 Brilliant Violet (BV)750 (BioLegend), CD3 BV421 (BioLegend) ...
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bioRxiv - Molecular Biology 2021Quote: ... Aβ was detected using clone 6E10 Aβ-specific antibody (BioLegend Cat# 803017, RRID:AB_2565327) and α-mouse Alexa Fluor 568 (Molecular Probes Cat# A-11004 ...
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bioRxiv - Immunology 2019Quote: Antibodies used in this study included PE anti-human CD45 (BioLegend, Cat#304008), PerCP-CyTM5.5 Mouse Anti-Human CD56 (BD ...