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Citations for BioLegend :
3201 - 3250 of 3260 citations for Recombinant Human ITGAV & ITGB5 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the cultured cells were harvested and incubated with either fluorescein isothiocyanate (FITC)-conjugated anti-human FR-β antibody (m90916, a gift from Philip S. Low) or allophycocyanin (APC)-conjugated anti-human FR-β antibody (mouse IgG2a, BioLegend, San Diego, CA, USA), and isotype controls (mouse IgG-FITC ...
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bioRxiv - Genomics 2019Quote: ... cells were washed once in PBS and once in Flow buffer (PBS with 2% BSA) and stained with anti-human CD47-APC antibody (clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3) in Flow buffer (20µl antibody/40×106 cells/ml ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of TNF-α mRNA and protein were detected by qPCR (Table 1) and ELISA (BioLegend, San Diego, CA), respectively.
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Pathology 2022Quote: ... was coated with 1.2 μg/mL HERV-K113 Capsid protein (sp|Q902F9|90-632, Genescript) diluted in ELISA coating buffer (421701, Biolegend) at 4 ℃ overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend, 101251, clone M1/70), CD45-Percp-Cy5.5 (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... Experimental values are expressed as a ratio of protein levels over control with the TFIIB (Biolegend, 1:2000 dilution) and Histone H3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... staining and neutrophil purity was assessed using a Benton Dickson LSR Fortessa X-20 flow cytometer via distinct forward and side scatter profiles or CD66b-peridinin chlorophyll protein (PerCP)/Cy5.5 (clone G10F5, BioLegend) expression ...
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bioRxiv - Genomics 2021Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend 101251, clone M1/70), CD45-APC (BioLegend 103112 ...
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bioRxiv - Bioengineering 2020Quote: ... recombinant AAV9 (2×109 vg/well) and SpCas9 protein (0.27 μg/well) were diluted in 1x Coating Buffer A (BioLegend) and used to coat a 96-well Nunc MaxiSorp plate ...
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bioRxiv - Cancer Biology 2022Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against IL-1α (503207, Biolegend), IL-1β (12426 ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Immunology 2022Quote: ... All enriched samples were stained with the following panel of TotalSeq-A-labeled anti-mouse antibodies to detect protein expression in the scRNA-seq dataset: Ly6C (HK1.4, BioLegend), CD44 (IM7 ...
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bioRxiv - Cell Biology 2024Quote: ... The expression level of specific proteins was detected by immunoblot analysis with the following antibodies: HA (BioLegend, MMS-101R), V5 (Thermo Fisher Scientific R96025) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunofluorescence on these tissues include: mouse anti-myelin basic protein (MBP) (1:1000; #808401 BioLegend), rabbit anti-MBP (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% BSA in Tris-buffered saline/0.05% Tween-20 and proteins were detected by specific primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), MOBP (bs-11184R ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... activated T-cells were treated with HDACI as described before and after 72 h total protein acetylation of the cells was determined by flow cytometry using PE-coupled anti-Acetylated Lysine Antibody (Biolegend). Cells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: ... The coronavirus protein panel consisted of the following flurorescent probes: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), WIV1 RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD20-APC for cells not incubated with protein or with anti-CD20-APC and anti-ROR1-PE (BioLegend) for samples treated with protein ...
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Immunology 2021Quote: ... we processed PBMC samples for scRNAseq with paired measurements of 192 surface proteins (CITE-seq, using TotalSeq reagents from Biolegend). The list of the antibodies included in the TotalSeq custom panel are detailed in Supplementary Data 12 ...
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bioRxiv - Immunology 2021Quote: ... Protein localization was confirmed by flow cytometry for either intact or fixed and permeabilized Intracellular Perm Buffer (BioLegend, Cat # 421002)) cells ...
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bioRxiv - Immunology 2020Quote: ... Protein levels in plasma and skin tissue protein extracts were measured using defined analyte panels: Legendplex Mouse Inflammation Panel and Inflammatory Chemokine Panel (Biolegend).
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Immunology 2023Quote: ... Nucleocapsid level on exosomes was measured by ELISA using precoated ELISA plates (Legend Max SARS-CoV2 Nucleocapsid protein ELISA Kit, 448007, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) for 4 hrs or Cell Activation Cocktail (Biolegend) in the presence of Brefeldin A and Monensin for 5 hrs before staining.
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bioRxiv - Genomics 2022Quote: ... The custom oligos were synthesized by Integrated DNA Technologies and conjugated to anti-Nuclear Pore Complex Proteins antibody (Clone Mab414, BioLegend) by BioLegend ...
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bioRxiv - Plant Biology 2024Quote: ... The protein levels were detected by immunoblot analysis using the primary antibodies: anti-HA antibody (Biolegend, #901503, dilution 1:5000), anti-FLAG antibody (CST ...
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bioRxiv - Neuroscience 2024Quote: Aggregated α-synuclein was quantified in PBS- and TrX-soluble tissue fractions (5µg total protein/well) using the LEGEND MAXTM α-Synuclein Aggregate ELISA (BioLegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... for Fc receptors blocking and then simultaneously incubated with HpARI proteins tetramers and stained for CD45 (APC-Cy7, clone 30-F11; Biolegend). Cells were then acquired on a LSR Fortressa (BD ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting homogenate was immunodepleted to remove soluble amyloid beta or mock immunodepleted using protein A agarose beads (Thermo, 20334) with 4G8 antibody (Biolegend, 800703) or mouse serum (Merck ...
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bioRxiv - Immunology 2020Quote: ... YU2-gp140 fold-on, and hepatitis B surface antigen (HBs Ag) (Protein Specialists, hbs-875) conjugated to streptavidin Alexa Fluor 647 (Biolegend, 405237), streptavidin PE (BD biosciences ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Neuroscience 2022Quote: Global protein concentration of the BV2 cells post treatment was assessed via flow cytometry using intracellular protein staining using the True-Nuclear Transcription Factor Buffer Set (Biolegend # 424401). Cells were incubated with primary antibodies for 30 minutes - 1:100 Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Immunology 2023Quote: ... HTO stained PBMCs from all 10 donors were then pooled and stained with oligo-tagged TotalSeq C antibody for cell surface proteins for T cell memory markers (CD45RA, CCR7, CD95 and IL7Rα) (0.5 µg/condition; Biolegend, Table S2B) for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b+CD45.2+CX3CR1+ microglia were sorted into a pre-cooled 5ml low-protein binding falcon tube (fisher scientific; Cat#: 13-864-407) containing 0.5ml cell staining buffer (BioLegend, Cat#: 420201). Walls of the tubes were washed and cells pelleted at 300g ...
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bioRxiv - Immunology 2023Quote: To determine galectin-3 or galectin-8 expression the following: intracellular protein and lysosomes were stained using anti-Lamp1-BV421 (clone H4A3, BioLegend, USA), anti-Galectin-3-AF488 (clone B2C10 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation with primary antibodies (rat anti-human α-synuclein, 15G7, 1:100 Enzo Life Sciences; mouse anti-α-synuclein [Syn1, clone 4D6], 1:1000, 834301, BioLegend; rabbit anti-GAPDH 1:2000 (D16H11) Cell Signaling Technologies ...