Labshake search
Citations for BioLegend :
3201 - 3250 of 3262 citations for Lysozyme C LYZ Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: ... cells were washed once in PBS and once in Flow buffer (PBS with 2% BSA) and stained with anti-human CD47-APC antibody (clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3) in Flow buffer (20µl antibody/40×106 cells/ml ...
-
bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
-
bioRxiv - Immunology 2021Quote: Isolated cells were stained at 4°C in FACS buffer (PBS supplemented with 2.5% bovine serum albumin) with and hematopoietic lineage markers including Ly6G (BioLegend, clone 1A8, 1:600), CD11b (BioLegend ...
-
bioRxiv - Molecular Biology 2021Quote: ... blotted membranes were blocked with 5% milk in TBST for 30 minutes and probed overnight (4°C) with primary antibody (anti-β-actin [Sigma-Aldrich, A5441], anti-HA [Biolegend, 901501]). After 3 washes with TBST ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... PE-Cy7 conjugated anti-rat CD11b/c (clone OX-42, Cat#201818), APC-Cy7 conjugated anti-rat CD4 (clone W3/25, Cat#201518) from Biolegend (San Diego, USA), and AF647-conjugated anti-rat RT1B (clone OX-6 ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.05% Sodium Azide in PBS) and incubate overnight at 4°C with the monoclonal anti-amyloid beta antibody 6E10 (Biolegend, 803001, lot. B228658) at a concentration of 3μg/ml (Antibody buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
-
bioRxiv - Immunology 2019Quote: ... slides were incubated for 50 min at 25 °C with different combinations of the following anti-mouse Abs (Thermo Fisher Scientific, Biolegend, BD Biosciences): Alexa Fluor 488-labeled anti-CD3 (HM3420 ...
-
bioRxiv - Systems Biology 2019Quote: ... We washed the samples again with 0.1% BSA-PBS and then incubated for 10 minutes at 4°C with 1:250 anti-NGFR antibody conjugated directly to PE-Cy7 (Biolegend, 400126, clone ME20.4) in 0.5% BSA-PBS with 2mM EDTA ...
-
bioRxiv - Immunology 2019Quote: ... Cell suspensions were incubated for 10 minutes at 4°C with 10μg/ml Fc Block (purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300 ...
-
bioRxiv - Immunology 2019Quote: Cells were incubated for 10 minutes at 4°C with 10μg/ml of purified rat anti-mouse CD16/CD32 (Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience ...
-
bioRxiv - Neuroscience 2019Quote: ... The triangularis sterni muscle was dissected and incubated overnight (4°C) in anti-βIII-tubulin antibody conjugated to Alexa488 (BioLegend 801203; 1:200) and Alexa594-conjugated α-bungarotoxin (Invitrogen B13423 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 30 min at 4°C with pre-conjugated fluorescent labeled antibodies with the following combinations: CD45 (BioLegend, 368532 (PE/Cyanine7)) ...
-
bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
-
bioRxiv - Genomics 2021Quote: ... Sections were incubated with the following primary antibodies at the indicated dilution in blocking buffer overnight at 4°C: NeuN (BioLegend 1B7, cat #834502), p65 (BioLegend Poly6226 ...
-
bioRxiv - Immunology 2021Quote: ... Spike and RBD tetramers were synthesized by incubating biotinylated protein with fluorophore-conjugated streptavidin overnight at 4°C (streptavidin-PE, Tonbo, cat. no. 50-4317-U100, streptavidin-APC, Tonbo, cat. no. 20-4317-U100, streptavidin-BV421, BioLegend, cat. no. 405226) at a molar ratio of 5:1 ...
-
bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
-
bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
-
bioRxiv - Immunology 2024Quote: ... and incubated at 37°C for 30 min with either a 1/500 dilution of PE-labeled anti-6xHis tag mouse MAb (Biolegend, San Diego, CA) to detect VHHs or Alexa Fluor 647-labeled goat anti-human IgG (H+L ...
-
bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
-
Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... Cells were incubated with a TotalSeq-C antibody cocktail as well as hashtag antibodies to allow for post sequencing differentiation of distinct expanded ILC subsets(BioLegend, Supplemental Table 1) for 30min ...
-
bioRxiv - Immunology 2024Quote: ... Surface proteins were then stained at 4°C for 30 minutes using the following markers: NIR Zombie dye APC-Cy7 (Biolegend, Cat No. 423105), CD20 PE (Biolegend ...
-
bioRxiv - Immunology 2023Quote: ... peripheral blood or BM were incubated for 20 min at 4°C with the following anti-mouse conjugated antibodies: anti-CD45-PerCP/Cy5.5 (BioLegend, clone 30-F11, #103132), anti-CD45.1-PE/Cyanine7 (BD Biosciences ...
-
bioRxiv - Biophysics 2023Quote: ... we incubate 5-6*105 NK92 cells prior to mixing them into the collagen for one hour in serum-free cell culture medium at 37°C with or without the following antibodies: 10 μg/ml anti-integrin activating β1 (CD29) antibody (Biolegend, cat. no. 303002) and the corresponding IgG1 isotype (Biolegend ...
-
bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with the following primary antibodies and dilutions: anti-Nestin (1:150, Biolegend, #656802, clone 10C2), anti-EGFR (1:300 ...
-
bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized with ice-cold methanol at −20°C for at least 20 min and incubated with anti-Pol II antibody (Biolegend, 8WG16 at 1:200), anti-PABPC antibody (Abcam ab21060 at 1:200 for mouse cells and Santa Cruz sc32318 at 1:25 for human cells ...
-
bioRxiv - Cancer Biology 2019Quote: ... Sigma)for one hour at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (BioLegend, San Diego, CA, USA), anti-Cytochrome c ...
-
bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated for 1 hour at 37 °C with different primary antibodies: anti-β-III-tubulin (1:4000, 801202, Biolegend, San Diego, CA), anti-neurofilament SMI312 (1:1000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... for 5 min at 37°C and erythrocytes were removed by incubating the cell suspension with red blood cell lysis buffer (Biolegend, 420301, San Diego, US) for 5 min at RT ...
-
bioRxiv - Immunology 2022Quote: ... Staining was then performed at 4°C for 30 minutes with a surface stain cocktail containing the following antibodies: CD4 APC (BioLegend, cat. 100412, clone GK1.5), CD8 Alexa Fluor 700 (BioLegend ...
-
bioRxiv - Cell Biology 2019Quote: ... Slides were double immunostained by incubating overnight at 4°C with a mix of anti-CD4 conjugated with PERCP (BioLegend, San Diego, CA, USA) and anti IL-10 conjugated with PE (BD Biosciences Pharmingen ...
-
bioRxiv - Immunology 2021Quote: ... Cells were suspended in FACS buffer and stained for 30 min at 4 °C with the desired antibody cocktails (BioLegend, San Diego, CA, US) of APC-Fire 750-anti CD11b (Cat ...
-
bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2 million cells for 30 min at 4 °C (Cat. No. 394601, 394603, 394605 and 394661, 394663, 394665, respectively, Biolegend, San Diego, CA, USA). After 3 washes with PBS with 0.05 % BSA respective cell suspensions were mixed prior to single-cell RNA library preparation ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissue was incubated in primary antibody overnight at 4°C in a moist chamber diluted in blocking buffer with the following dilutions: Anti-Tubulin β3 (BioLegend, clone TUJ1, 1:500); Anti-OMP (Santa Cruz ...
-
bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were then stained for 30 minutes at room temperature with a panel of 138 Total-Seq-C antibodies (Biolegend, Stoeckius et al 2017) and washed 3x using the HT1000 laminar wash system (Curiox) ...
-
bioRxiv - Immunology 2022Quote: ... 4 °C) cells were stained with the following conjugated antibodies for 20 min at RT in the dark: CD3-APC (Biolegend, clone REA613, 1:200), CD11b-APC-Cy7 (Biolegend ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250, pS129 α-synuclein (81A, Biolegend 825701, 1:1000).
-
bioRxiv - Cell Biology 2024Quote: ... A C-terminal fragment of Sch9–5HA was detected using a mouse monoclonal anti-HA antibody (AB2565007, cat. # 901502; BioLegend, San Diego, CA, USA). Atg13 was detected using an anti-Atg13 antibody gifted by Dr ...
-
bioRxiv - Immunology 2023Quote: ... the cells were stained for 20 minutes at 4 °C using a Cyto-Fast™ Fix/Perm Buffer Set (426803, BioLegend; 100 μL/tube). After being washed and resuspended with Cyto-Fast™ perm wash solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with DAPI and the following antibodies and dilutions: Alexa Fluor® 488 anti-Vimentin (1:500, Biolegend, #677809, clone O91D3), anti-Ki67 (1:300 ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were stained (30 min, 4°C in the dark) with CD11b (Clone: REA713; Miltenyi or Clone: M1/70; Biolegend, San Diego, CA, USA), CD14 (Clone ...
-
bioRxiv - Immunology 2021Quote: ... and stained by intracellular staining for 30 min at 4 °C using a cocktail of the following antibodies: IFN-γ PE (Biolegend, Clone W18272D,1:500), TNF Percp-Cy5.5(Biolegend ...
-
bioRxiv - Developmental Biology 2021Quote: ... the cells were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Single cell suspensions were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE ...
-
bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...