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Citations for BioLegend :
3151 - 3200 of 3691 citations for Recombinant Human TNFRSF18 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies that were used in this study included rabbit anti- human CD271 (1:100, HPA004765, Biolegend), mice anti-human CXCL12 (1:50 ...
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bioRxiv - Immunology 2023Quote: Cytokine analysis from co-culture supernatants was performed with Human CD8/NK Panel (13-plex) Cat#741065 (BioLegend) following the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Dynabeads M270-Epoxy (Thermo) was covalently conjugated with combinations of mouse anti-human CD3 antibody (clone UCHT1, BioLegend), mouse anti-human CD28 antibody (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Immunology 2023Quote: ... sorted TN and TEMRA cells were stimulated with plate-bound anti-human CD3 (OKT3; 1 μg/ml; BioLegend) and anti-human CD28 (CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... slides were incubated with primary antibodies to human phospho-6SRP (Ser235/236) (CellSignaling 4858) or CD45 (BioLegend 304002) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (1x106) were stained with PE-labelled anti-human CD38 monoclonal antibody (clone HB-7, BioLegend Cat # 356604) in FACS buffer (0.5% FBS with 2 mM EDTA ...
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bioRxiv - Bioengineering 2024Quote: ... TCR was detected using an anti-human TCR α/β antibody conjugate to BV421 (Biolegend, San Diego, CA). Flow cytometry to assess CAR and TCR positivity was performed on Day 8 of manufacturing on an Attune NxT flow cytometer (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... cells were pretreated with an FcR blocker (Miltenyi Biotechnology, #130-092-575 for mouse; BioLegend, #422302 for human) for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... 5 μl PE-labeled mouse anti-human CD11b fluorescent antibody (cat. no. 301306; 1:1,000 dilution; BioLegend, Inc.) or FITC-labeled mouse anti human CD14 fluorescent antibody (cat ...
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bioRxiv - Neuroscience 2024Quote: ... Macrophages were instead stained with 5 ul of either APC/Cy7-anti human CD16 antibody clone 3G8 (Biolegend), APC-anti human CD206 antibody (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-dephosphorylated neurofilament protein (clone smi32; Biolegend, San Diego, CA, USA); rabbit anti-Caspr1 (clone EPR7828 ...
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bioRxiv - Immunology 2022Quote: ... −phycoerythrin (PE) and −peridinin chlorophyll protein complex (PerCP) were purchased from Biolegend. Phospho-specific mAbs against p65 (S536 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies against the following proteins and epitopes were used: HA (16B12, BioLegend), β-actin (AC-74 ...
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bioRxiv - Bioengineering 2024Quote: The scFc protein was labeled with SNAP-Surface 488 (BG-488, Biolegend). In brief ...
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...
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bioRxiv - Genomics 2022Quote: ... Proteins of interest were visualized using an anti-Flag-PE (BioLegend, 637310), an anti-HA-Alexa647 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilised with 0.2 % Triton X in PBS and probed using anti-human CD63 antibody (Clone: H5C6, Biolegend Cat: 353005) directly conjugated to FITC and counterstained for polymerised actin using 0.2 × Alexa Fluor 555 Phalloidin (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellet was further resuspended in 150ul PBS+0.5%BSA and stained with PE anti-human CD56 antibody (1:100, Biolegend) for 20min in dark ...
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bioRxiv - Immunology 2021Quote: Supernatants were tested for multiple cytokine production using the bead assay LEGENDplex Antivirus Human panel (Biolegend, San Diego, USA) or with a commercial Luminex multi-analyte assay (Biotechne ...
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bioRxiv - Immunology 2021Quote: ... in PBS) for 30 minutes with 1:400 dilution of FITC Mouse anti-Human HLA-A2 (clone BB7, Biolegend), APC anti-human HLA-A,B,C (clone W6/32 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with Brilliant Violet 421-labelled anti-human P2Y12 receptor antibody (clone S16001E, Biolegend, Cat# 392106) or with IgG2a isotype control antibody (clone MOPC-173 ...
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bioRxiv - Immunology 2021Quote: ... Secreted cytokines were measured with ELISA MAX Standard Set Mouse or Human kits according to the manufacturer’s protocol (BioLegend). Cellular viability was determined by resuspending cells in cRPMI with 0.1mM resazurin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were analyzed using the LEGENDplex™ Human Anti-Virus Response Panel multiplex assay (BioLegend, San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using anti-human/mouse □7 integrin antibody (FIB504, Biolegend) and the EasySep Biotin Positive Selection Kit (Stemcell Technologies ...
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bioRxiv - Systems Biology 2019Quote: ... and IL-6 secreted protein level in cell supernatant from 1×106 human MoDM’s was performed by Legend Plex multi-analyte assay kit (BioLegend) according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2019Quote: ... IHC was performed to rat or human tissue with the following panel of antibodies: pan-Aβ (Biolegend, Cat.# 800709,), pE3-Aβ (Cell Signaling Technologies ...
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bioRxiv - Immunology 2021Quote: ... either 100 µl 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once in PBS+0.2% BSA and stained for 30 min with anti-human STAT3 pTyr705-Alexa Fluor 647 (BioLegend) in PBS+0.2% BSA at RT ...
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bioRxiv - Bioengineering 2020Quote: ... The remaining cellular content was stained with anti-human CD45 (PacBlue or PECy7, clone HI30, BioLegend #304029 or #304016) and biotinylated cetuximab ...
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bioRxiv - Neuroscience 2020Quote: ... APC-conjugated rat monoclonal anti-mouse CD11b and PerCP-Cy5.5-conjugated mouse monoclonal anti-human CD200 (all Biolegend, UK) on ice in the dark for 30 min ...
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bioRxiv - Cell Biology 2021Quote: Whole blood or washed platelets were labelled with anti-human BV421 CD62p (P-selectin; 1/100, AK4, BioLegend, 304926), BV421 CD42b(1/300 ...
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bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Immunology 2022Quote: ... and the cells were resuspended in 200 μL of PBS supplemented with 1% FBS and containing 2.5 μL of Alexa Fluor 647-conjugated anti-human CD127antibody (BioLegend) or Alexa Fluor 647-conjugated mouse IgG1 k isotype control (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... Antigen experienced CAR T cells were stained for CD69 expression using APC-conjugated anti-human CD69 antibody (Biolegend #310910). Flow cytometric data was acquired using a BD LSRFortessa with BD FACSDiva software ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
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bioRxiv - Genomics 2019Quote: ... CD4+ T helper cells were sorted as naive T cells (CD4+CD25−CD45RA+) or memory T cells (CD4+CD25−CD45RA−) using the following antibodies: anti-human CD45RA-PERCPCy5.5 (Clone HI100, Biolegend), anti-human CD4-APC-Cy7 (Clone OKT4 ...
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bioRxiv - Genomics 2019Quote: ... Cells were labelled for CD47 expression with biotin anti-human CD47 antibody (clone CC2C6, BioLegend, Cat.# 323104, RRID: AB_756134) at 0.0625 µg/100 µl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was then blocked with 10% normal donkey serum/0.1% Triton X- 100/0.01% NaN3 followed by staining with Alexa Fluor 488 conjugated anti-human CD4 OKT 4) antibody (Biolegend) at 4° Celsius overnight (1:200 diluted in PBS) ...
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bioRxiv - Bioengineering 2021Quote: ... Enriched EPCs from Day 10 were examined by staining with APC Anti-Human CD71 antibody (334107; Biolegend; 1:100) and incubating for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and DAPI/conjugated PECAM1 antibody (0.04 µg/ml DAPI, Sigma, 10236276001; 1:250 Alexa647-anti-human PECAM1, Biolegend, 303112) in blocking buffer for 1 hr each ...
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bioRxiv - Cell Biology 2021Quote: ... samples were washed with FACS buffer (0.3% BSA in PBS) and stained with Human TruStain FcX (BioLegend, Cat# 422301) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used to stain for IL-4Rα were APC anti-human CD124 (IL-4Rα) (Biolegend, clone G077F6, 1:100) and Live/Dead Fixable Green (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used for intracellular staining: Anti-human TBET FITC (1:50, clone 4B10, BioLegend, cat # 644812), Anti-human Granzyme B FITC (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... Alexa Fluor 647-labelled monoclonal mouse anti-human CD62P (P-Selectin) antibody (Clone AK4, BioLegend, San Diego, CA, USA), Alexa Fluor 647-labelled goat anti-mouse IgG (GAMIG AF-647 ...
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bioRxiv - Immunology 2020Quote: ... and Il6) in treated PBMC supernatants were quantified by ELISA using the ELISA MAX Deluxe Set Human CCL2 (Biolegend), ELISA MAX Deluxe Set Human IL8 (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... Bacteria were opsonised in heat-inactivated human serum for 30 minutes at 37°C and labelled via incubation with 10μM carboxyfluorescein succinimidyl ester (CFSE) (Biolegend) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako; and FITC-Annexin V from Biolegend) for 20 min at RT and centrifuged on 45° fixed angle rotor FA-45-30-11 (Eppendorf ...