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Citations for BioLegend :
3151 - 3200 of 10000+ citations for Anti Syndecan 4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF) 488 mouse anti-human BAX antibody (0.5 μg/mL, Biolegend, Cat. No. 633603) or AF488 Mouse IgG1 κ isotype control (5 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... Pelleted nuclei were suspended in Nuclear Stain Buffer (Sucrose Buffer containing 1:4,000 Alexa Fluor® 647 anti-Nuclear Pore Complex Proteins Antibody (BioLegend, Cat# 682204) plus 4µM ethidium homodimer (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Developmental Biology 2023Quote: ... GAPDH was used as an internal control (1:1000, Direct-Blot™ HRP anti-GAPDH antibody, 607903; Biolegend, San Diego, CA, USA). Signals were visualized using Luminata Forte (Merck Millipore ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Immunology 2024Quote: ... CD19-expressing K562 (CAR Activation) or CD64- and CD80-expressing K562 previously loaded with anti-CD3 antibody (OKT3, Biolegend, San Diego, CA) at 1 μg/ml for 1 hour 39 (TCR/CD28 Activation ...
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bioRxiv - Neuroscience 2023Quote: ... were rehydrated in distilled H2O then stained with Nissl or immunohistochemical labelling was performed as above with anti-mouse Ly6G primary antibody (BioLegend, London, UK) and goat anti-rabbit secondary antibody.
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bioRxiv - Cell Biology 2023Quote: ... The protein mixture was then added to 800-nm diameter carboxylated polystyrene beads that were coated with a rabbit polyclonal anti-GFP antibody (BioLegend MMS-118P), and incubated for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used and the dilutions were as follows: for neurons: anti-Tubulin-βIII Rabbit mAb (1:200, BioLegend, 802001, clone TUJ1); anti-Synapsin-1 Mouse mAb (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated at 4degC with the following fluorochrome conjugated primary anti-mouse antibodies for 45 minutes (all Biolegend, San Diego, CA): anti-CD45 PE-CF594 (clone 30-F11) ...
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bioRxiv - Bioengineering 2023Quote: ... the CNP labeled cells were spin washed and incubated with 10μgꞏml−1 of AF488 conjugated anti-CD3 antibodies (Biolegend, Cat # 300415, clone UCHT1) in 2% BSA/PBS on ice for 30min and then spin washed ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor single-cell suspensions were stained with Alexa Fluor 700 anti-CD326 (Ep-CAM) antibody (Biolegend, 118239, clone: G8.8, 1:100 dilution), PE/Cyanine 7 anti-mouse CD31antibody (Biolegend ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of PRP was incubated with 1 μg/mL AF647-conjugated CD42b rabbit anti-human antibody (BioLegend, San Diego, CA, USA) to label the platelets ...
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bioRxiv - Immunology 2023Quote: Freshly sorted CD8+ T lymphocytes of four healthy volunteers were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for the period of activation expansion with 20µg/ml of anti IL-10 antibody (JES5-2A5; Biolegend catalog No. 504908) or corresponding isotype control (Biolegend catalog No ...
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bioRxiv - Cancer Biology 2023Quote: ... This additional step removes the likelihood of TCR α-chain and β-chain mispairing and allows us to evaluate TCR-transduction efficiency by staining with anti-murine TCR constant region antibody (Clone H57-597; BioLegend Cat. #109208). Flow sorting was performed to isolate highly-transduced CD8+ T-cells by selecting for cells stained strongly with anti-CD3 and anti-murine TCR constant region antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell suspension from mouse tumors or spleens were stained with Alexa Fluor 700 anti-mouse CD45 Antibody (Biolegend, USA, Cat#147716), APC anti-mouse F4/80 Antibody (Biolegend ...
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bioRxiv - Biophysics 2023Quote: Antibodies uptake was determined by measuring the fluorescent intensity of a fluorescently-tagged antibody (Alexa Fluor® 647 anti-Tubulin-α, 0.5 mg/mL, BioLegend 627908) at a dilution ratio of 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... moDCs were also stained for surface markers using BioLegend anti-human antibodies for monocyte and dendritic cell associated surface markers: CD14 (M5E2; BioLegend, Cat# 301814), CD11c (B-ly6 ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor® 700 anti-human CD41 antibody and Zombie NIR® fixable dye were obtained from BioLegend (San Diego, CA, USA). Anti-human CD62P conjugated PE antibody was obtained from Beckman Coulter (Brea ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used for flow cytometry: Brilliant Violet 510-conjugated anti-CD19 (clone RA3-6B2, BioLegend, San Diego, CA, USA), phycoerythrin (PE)-Cy7-conjugated anti-CD3ε (clone 145-2C11 ...
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bioRxiv - Microbiology 2024Quote: Suspensions of ∼106 HIV-infected TMCs in PBS with 1% FBS were incubated with BV711 anti-human CD3 antibody (Biolegend, catalog # 300464) at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with the following antibody cocktail diluted in flow cytometry staining buffer: mouse anti-human CD3 BV750 (BioLegend Cat 344846), mouse anti-human CD4 AF700 (BioLegend Cat 300526) ...
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bioRxiv - Neuroscience 2024Quote: ... cell preparation and cell surface marker staining was carried out as previously described.20 The following antibodies were used for staining: mouse anti-CD11b (Biolegend, Cat# 101257), mouse anti-CD19 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... IL-4-APC (clone MP4-25D2, BioLegend Cat# 500812, RRID:AB_315131), IL-17A-AlexaFluor488 (clone eBio64Dec17 ...
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bioRxiv - Immunology 2019Quote: ... 4 were analyzed with the following cocktail: CD4-BV650 (Biolegend), CD25-BV605(Biolegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 4 hours with Brefeldin A (Biolegend) and Monensin (Sigma Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and stained with CD11b-APC (M1/70, Biolegend), CD19-PE (HIB19 ...
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bioRxiv - Immunology 2020Quote: ... CD8-FITC and 4-1BB-CD137 APC (1/100, BioLegend). Cells were incubated at 37°C for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Tbet BV605 (clone 4B10, BioLegend, cat# 644817, dilution 4:100); Bcl6 PE/Dazzle (clone 7D1 ...
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bioRxiv - Genomics 2022Quote: ... 50 ng/mL of recombinant IL-4 (BioLegend, Cat# 574302) and 1 μg/mL of soluble anti-CD28 for 6 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and stained overnight at 4°C with avidin-FITC (BioLegend), anti-human/mouse CD117 (c-kit ...
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bioRxiv - Microbiology 2022Quote: ... CD137 (4-1BB) PE (2.5 μg/mL, BioLegend Cat. 106106), and CD197 (CCR7 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then fixed in 1x Fixation Buffer (4% paraformaldehyde, BioLegend). Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF ...
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bioRxiv - Cancer Biology 2024Quote: ... Panel 4: APC/750-conjugated CD45 (1:100, 147713, Biolegend), BV421-conjugated CD3 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/ml of recombinant IL-4 (Cat# 574302, BioLegend), and 10 ng/ml of recombinant IL-13 (Cat# 575902 ...
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bioRxiv - Immunology 2023Quote: ... and IL-4 (20 ng ml−1, BioLegend Cat# 574004) (Mo-DCs ...
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bioRxiv - Microbiology 2023Quote: ... 4°C) and stained with CD11b-APC (M1/70, Biolegend), CD19-PE (HIB19 ...
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bioRxiv - Immunology 2023Quote: ... IL-4 (11B11) and IL-5 (TRFK5) were from Biolegend. Following surface and/or intracellular stain ...
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bioRxiv - Immunology 2023Quote: ... and 10 ng/mL of recombinant mouse IL-4 (BioLegend) diluted in PBS was added to wells cultured with the cytokine ...
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bioRxiv - Immunology 2024Quote: ... and IL-4 (20 ng/ml, Biolegend, San Diego, USA). Cells were allowed to differentiate for 5-7 days (DC-SIGN+ cells in the culture > 90 %).
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bioRxiv - Cancer Biology 2021Quote: ... antibody and the isotype control antibody (PE Mouse IgG2b, Biolegend, catalog#:400314) has a maximum excitation at 575 nm ...
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bioRxiv - Microbiology 2022Quote: ... All antibodies and isotype matched control antibodies are commercially available (BioLegend, Invitrogen).
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bioRxiv - Immunology 2024Quote: ... cells were stained with both flow cytometry antibodies and TotalSeq antibodies (BioLegend) as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... Flow Cytometry primary antibodies and working dilutions: BV421-CD19 antibody (BioLegend, #115537); PE-CD276 (1:100 ...