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Citations for BioLegend :
3101 - 3150 of 3250 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for T cells: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD26 PerCP/Cy5.5 (BioLegend catalog #356112 ...
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bioRxiv - Immunology 2024Quote: Concentrations of IFN-γ in mouse plasma and ex vivo stimulation supernatants were determined using the ELISA MAX Deluxe Set Human IFN-γ Kit (BioLegend, cat #430104) according to the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1/20 for secondary mouse anti-human Ig light chain K antibody Alexa Fluor® 647 (316514, BioLegend®, San Diego, CA, USA) and mixed with Hoescht 33342 (H3570 ...
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bioRxiv - Immunology 2021Quote: ... Bio-PyS23 protein (44.8 kDa) at 16 μM was combined with SA-APC/Fire750 antibody (Biolegend) at 2 µM and was used as a decoy ...
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bioRxiv - Immunology 2020Quote: ... Samples were normalized to 0.5 mg/mL protein in Legendplex assay buffer (Biolegend, San Diego, CA) using a Bradford Assay (BioRad ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell supernatants were collected to monitor IFNG protein expression by ELISA according to manufacturer protocols (BioLegend). Antibodies used ...
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bioRxiv - Developmental Biology 2024Quote: ... labeled with 1 μg of a unique TotalSeq anti-Nuclear Pore Complex Proteins Hashtag Antibody (Biolegend), and then incubated on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative analysis of secreted proteins was performed using a LEGENDplexTM Custom Mouse 14-plex Panel (BioLegend).
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were labeled with primary antibodies against CD9 (1:1,000; clone HI9, Biolegend, San Diego, USA), CD44 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µl of TotalSeqB Hashtag (anti-Nuclear Pore Complex Proteins Hashtag at 0.5g, Biolegend 682239-682243) was added to each sample for subsequent identification in downstream analysis ...
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bioRxiv - Neuroscience 2024Quote: ... with the molecular weight marker (Prime-Step Prestained, broad range protein Ladder, BioLegend, San Diego, CA) in MES-SDS running buffer (Genscript ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by western blotting using an anti-RNAP β subunit antibody [clone 8RB13] (BioLegend), anti-σ70 antibody [clone 2G10] (BioLegend) ...
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bioRxiv - Cell Biology 2024Quote: ... Blocked embryos were incubated with the mouse anti-Nuclear Pore Complex Proteins (1:200, BioLegend, #902901) antibody in PBSTw supplemented with 5% NGS (Normal Goat Serum ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Cancer Biology 2022Quote: ... was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (Biolegend, San Diego, CA, USA). Mice were monitored closely to detect disease onset and treatment started when hCD45+ cells were detectable in the peripheral blood ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were blocked with 0.5 % BSA for 10 min on ice and incubated with the primary mAbs biotin-anti-human CD274 (B7-H1, PD-L1) (clone 29E.2A3, BioLegend, San Diego, CA, USA) or biotin anti-mouse CD274 (clone 10F.9G2 ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 x with 1% BSA before incubating for 1 h in anti-human IgG secondary antibody labelled with either APC (BioLegend, #410712, dilution 1:100) or Phycoerythrin (Abcam ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were spun down to remove cell debris and frozen at -80 °C until analysis using the ELISA MAX™ Deluxe Set Human IFN-γ (BioLegend catalog #430104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Blocking of CD47 was performed with purified mouse anti-human CD47 mAb (αCD47-fuFc) clone CC2C6 (cat. no. 323102, Biolegend, San Diego, CA, USA), and humanized anti-CD47 IgG2σ mAb (αCD47-siFc ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 15 minutes at 4 °C in the dark with a combination of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend, San Diego, CA, USA) and Live/Dead™ (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Ultra-LEAFTM purified anti-human IL-10 mAb (JES3-9D7) and 7-Aminoactinomycin D (7-AAD) were purchased from BioLegend (San Diego, CA, USA). Anti-IgA mAb (mA-6E1 ...
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bioRxiv - Biochemistry 2024Quote: ... Five- micron thick sections of formalin-fixed paraffin-embedded human tissue from AD and PD patients were used to perform immunohistochemistry for Phosphorylated α-Synuclein (pSyn) (Biolegend Cat. No. 825701) and Pan-Amyloid beta (Aβ ...
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bioRxiv - Cancer Biology 2024Quote: 2*105 N87 and N87-R cells were incubated on ice for 30 min with PE anti-human HER2 antibody (Biolegend, clone 24D2, cat#324406), APC anti-human ABCG2 antibody (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... in a protein low-binding microcentrifuge tube separately and incubated with 5 μL Fc blocking reagent (BioLegend) for 10 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were normalised to protein concentration before being subject to an ELISA protocol or TNF-α (Biolegend), IL-12 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and detection of indicated proteins was carried out using the following antibodies: Eos (W15169A; BioLegend, 1:500), pY-STAT5 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: Oligonucleotides were conjugated to two antibodies against HGSOC cell surface proteins β2-Microglobulin (β2M) and CD298 (BioLegend) by iEDDA-click chemistry according to CITE-seq antibody-oligo conjugation protocol (https://cite-seq.com) ...
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bioRxiv - Immunology 2022Quote: ... TNF-α and IL-6 protein were quantified using ELISA MAX Deluxe kits (BioLegend, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Blocking antibodies specific for the following proteins were used: 2.5 μg/ml CD11b (M1/70, BioLegend, #101224), 2.5 μg/ml CD11c (N418 ...
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bioRxiv - Cancer Biology 2023Quote: CD41 and CD62p proteins on platelets were stained with FITC conjugated anti-CD41 (BioLegend 303704,1:50 dilution) and PE conjugated anti-CD62p (BD Pharmingen 561921 ...
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bioRxiv - Immunology 2023Quote: ... The gels were transferred to nitrocellulose and Mab-associated proteins were detected with anti-C3 (BioLegend, 846302), -C1q (Novus ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated for the following 5h using a combination of protein transport inhibitor (Monensin) (Biolegend, USA), Ionomycin (1 ug/ml) ...
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bioRxiv - Immunology 2024Quote: Mouse IL-10 and IL-1β proteins were measured in cell culture supernatants using kits from Biolegend and Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...