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Citations for BioLegend :
3101 - 3150 of 8506 citations for 6 Amino 5 2 chloro 4 nitrophenyl azo naphthalene 1 sulfonamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Non-cardiomyocytes resuspended in Cell Staining Buffer (phosphate-buffered saline containing 2% FBS and 0.1% sodium azide) were treated with anti-mouse CD16/CD32 antibody (BioLegend, #101302) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from the spleen were prepared as described above and splenocytes were exposed to 2-μM Tag-it VioletTM Proliferation and Cell Tracking Dye (Biolegend) diluted in PBS supplemented with 0.1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... The antibody solution was then removed and the isolated lymph node cells were added at 2×105 cells per well together with soluable anti-mouse CD28 (102112, Biolegend) at a final concentration of 2 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... cells from the dermis and epidermis were combined and cultured overnight in the presence of 10 U/ml IL-2 (Biolegend). The following antibodies were used in flow analysis of skin associated γδ T cell populations ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 bone marrow cells were cultured at 37°C in 10ml bmDC culture medium (RPMI 1640 medium with 10% FBS, penicillin/streptomycin and 2-Mercaptoethanol) with 40ng/mL GM-CSF (BioLegend). At day 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in FACS buffer (PBS plus 2% FBS) and blocked with monoclonal antibody to CD16/32 (Human TruStain FcX, #422302, Biolegend) for 10 min at 4°C before staining with surface antibodies ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Genomics 2023Quote: ... Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e. stimulated) (Cell Activation Cocktail with Brefeldin A, Biolegend®), or DMSO as control (i.e ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Lower compartment of the transwell plate was loaded with 200 µl of X-VIVO-15 supplemented with 2 % HS and 100 ng/ml of CCL19 and CCL21 (#582102, #582202, Biolegend). To determine passive cDC2 migration ...
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bioRxiv - Microbiology 2023Quote: ... BMDMs were stimulated for 2 hours prior to infection with vehicle or 10 ng/mL of each cytokine: GM- CSF (Biolegend), M-CSF (Miltenyi) ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...
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bioRxiv - Immunology 2024Quote: ... samples were stained on ice for 45 minutes in PBS with 2% Newborn Calf Serum and True-Stain Monocyte Blocker (BioLegend) using a combination of the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... IL-2 (anti-human IL-2, PE-Cy7, clone MQ1-17H12), and CD154 (anti-human CD154, APC, clone 24-31) all from Biolegend in Perm wash buffer (PBS + 0.02% NaN3 + 0.5% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: Surface staining of lung single cell suspensions was performed in PBS with 2% Newborn Calf Serum on ice for 30 minutes with the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-IgD Pacific Blue (Clone IA6-2, 348224), anti-IgM BV570 (Clone MMH-88, 314517) and Zombie NIR fixable viability dye (423106, Biolegend). Cells were then washed in PBS (D8537 ...
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bioRxiv - Immunology 2024Quote: ... Sections were saturated with BSA 2% PBS and then incubated overnight with an APC-conjugated anti-CD45 antibody (clone 30F11, Biolegend) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: The single-cell suspension was stained at 4°C for 15 minutes with antibodies recognizing lymphocytes and endothelial cells (anti-CD45/CD31; BioLegend Way, San Diego, CA, USA) and labeling live cells (CellTrace calcein green ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC, Life Technologies; CD11b AF700, Life Technologies CD19 PerCP-Cy5.5, BioLegend; C33 BV605, BioLegend; CD56 BV711, BD). After 30min ...
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bioRxiv - Physiology 2019Quote: ... 100 μl cell suspension was transferred to the test tube and stained with 5 μl Pacific Blue Annexin V and propidium iodine (PI) solution (BioLegend, San Diego, CA) respectively for 15 min in dark ...
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bioRxiv - Bioengineering 2022Quote: ... supernatant was removed and nanovials were reconstituted at a ten-fold dilution in Washing buffer containing 5 μl of 100 μg/mL anti-IFN-γ BV421 (Biolegend, Catalog: 502532), 25 μg/mL anti-TNF-α APC (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... Nanovials were pelleted at the last wash step and incubated with 5 μl of 100 μg/mL anti-IFN-γ BV421 (Biolegend, Catalog: 502532), 25 μg/mL anti-TNF-α Alexa Fluor 647 (Biolegend ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells from healthy donors were activated in cell culture plates precoated with CD3 (5 μg/mL, BioLegend, San Diego, CA) and CD28 (1 μg/mL ...
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bioRxiv - Immunology 2022Quote: Primary human neutrophils isolated as described were stained at room temperature for 20 minutes with 5 μM Calcein AM (BioLegend, Cat. No. 425201) and applied to the top well of a fluorescence-blocking 24-well transwell plate with 3 μm pores (Corning ...
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bioRxiv - Immunology 2022Quote: ... Circulating maternal neutrophils were depleted at days 5 (E4.5) and 8 (E7.5) of pregnancy using a monoclonal neutralizing antibody (Biolegend, clone 1A8; 50 μg i.v.) or isotype control (Biolegend clone RTK2758 ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse IL4 (10 µg/mL) and recombinant mouse IL2 (5 ng/mL) and IL12 (10 ng/mL) (BioLegend, San Diego, CA, US), for three days prior to use.
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bioRxiv - Immunology 2023Quote: Biotinylated pneumococcal serotype 3 polysaccharide with a 5% biotin load was coupled with high concentration streptavidin-phycoerythrin (SA-PE) and streptavidin-allophycocyanin (SA-APC, both from BioLegend, San Diego, CA) in separate reactions ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were resuspended in 100 µL of Fc-blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody (Biolegend, San Diego, CA) in FACS buffer) ...
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies were anti-tau monoclonal antibodies including DA9 (aa 102–140, 1:10 dilution), ET3 (aa 273-288, 1:10 dilution)57 and 77G7 (BioLegend, aa 316-355, 1:100 dilution). DA9 and ET3 antibodies were generously provided by Peter Davies ...
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bioRxiv - Cancer Biology 2022Quote: ... PE-Cy7-conjugated anti-PDPN (8.1.1, #127411, 1:100), APC-Cy7-conjugated anti-MHCII (M5/114.15.2, #107628, 1:300) (from BioLegend); FITC-conjugated Hypoxyprobe-1-MAb1 (4.3.11.3 ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific antibody was detected using biotinylated anti-mouse antibodies (anti-IgG1 [RMG1-1] 0.02μg/ml, anti-IgG2b [RMG2b-1] 0.05μg/ml, Biolegend, CA ...
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bioRxiv - Immunology 2020Quote: ... CD64(FcγRI)#BV711 (1/100; 139311) and IFNγ#PE/Cy7 (1/300; 505825) were from Biolegend.
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bioRxiv - Biochemistry 2020Quote: ... anti-tubulin antibodies E7 (DSHB, AB_528499, 1:1000) and/or TU-01 (BioLegend 625902, 1:1000), and anti-RNA Polymerase II (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×106 CD34+ HSPCs or HSCs were stained with APC-Cy7-anti-CD34 (1:100) (BioLegend), PE-Cy7-anti-CD38 (1:50) ...
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bioRxiv - Immunology 2020Quote: ... followed by a 1 h incubation with a 1:3000 dilution of HRP-labeled streptavidin (Biolegend) in Casein ...
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bioRxiv - Cancer Biology 2019Quote: ... Gr-1 (1:100) (Purified anti-mouse Ly-6G/Ly-6c, 108401, BioLegend®,CA, USA), PDL-1 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 1 μg/mL anti-CD28 and 1 ng/mL recombinant IL7 (BioLegend) for 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... + 1% Glutamax + 1% Pen-strep + 0.1 ug/ml IL-34 (PeproTech, #200-34; Biolegend Inc, #577904) + 10 ng/ml GM-CSF (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... PE/Cy7 anti-mouse Ly-6G/Ly-6C (Gr-1) (cat#108415) (all BioLegend 1:20). Cryopreserved passage 1 human liver sinusoidal endothelial cells (LSEC ...
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bioRxiv - Developmental Biology 2022Quote: ... live GFP+ cells (Extended Data Fig.1; live stain 1:500 propidium iodide solution (BioLegend #421301)) were sorted on an FACSAria III (BD ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells expressing IgE were assessed using anti-Mouse IgE PE (1:200, BioLegend, 406908; RME-1). Live/ dead cells were discriminated after staining with Zombie Aqua viability dye ...
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bioRxiv - Bioengineering 2023Quote: ... anti-Ki67 (1:100, 14-5698-82, eBioscience™) and anti-CD31 (1:200, 102516, Biolegend). As secondary antibody Alexa Fluor 555 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were allophycocyanin (APC)-conjugated monoclonal rat anti-mouse IgG1 RMG1–1 (Biolegend 1:50) or goat anti mouse IgG poly4053 (Biolegend 1:50) ...
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bioRxiv - Neuroscience 2024Quote: ... and HashTag antibodies (1 µl for labeling ∼1 x 106 nuclei, BioLegend, TotalSeqB anti-Nuclear Pore) in order to enrich neurons and multiplex samples ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TIM-1 (BioLegend, catalog #353903), anti-TIM-4 (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... EpCAM-PE (1:500, Biolegend, 118206), MHCII-APC-eFluor-780 (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... anti-CD86-BV650 (GL-1, Biolegend), anti-CD80-PE-Dazzle (16-10A1 ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... anti-mouse IgG1 (RMG1-1, BioLegend), polyclonal Hamster IgG (BioXcell) ...
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bioRxiv - Immunology 2021Quote: ... PD-1 (clone 29F.1A12, BioLegend), CTLA-4 (clone UC10-4B9 ...
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bioRxiv - Immunology 2021Quote: ... chicken anti-GFAP (1/1500, Biolegend), mouse anti-NeuN (1/100 ...