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Citations for BioLegend :
3001 - 3050 of 7647 citations for Propane 1 2 Diyl Diacetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... mice were irradiated with a total of 1100 rad in two divided doses and injected intravenously with a 1:1 mixture of T cell-depleted (magnetic bead depletion; Thy1.2; BioLegend) bone marrow from CD45.1+CD45.2+ mice and control C57BL/6J (CD45.2+ ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with Live/Dead Yellow (1:1000) followed by Fc block (1:200; BioLegend, San Diego, CA), and stained with antibody cocktails for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... either 100 µl 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P ...
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bioRxiv - Developmental Biology 2022Quote: ... to block non-specific binding before being exposed to varying concentrations (0.25, 0.5, 1.0 and 2μg/μl) of PE β2M/PE CD298 (BioLegend, mixed 1:1) for 30 min in the dark at 4°C ...
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bioRxiv - Immunology 2022Quote: ... anti-granzyme B PE (1:50; clone QA16A02) and anti-TNFα BV711 (1:50; clone MP6-XT22) from BioLegend in permeabilization buffer at 4°C for 30 min ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD49a APC (1:50; clone HMα1) and anti-CD11b BV570 (1:100; clone M1/70) from BioLegend. Cells were fixed for 30 min at 4°C using eBioscicencesTM FoxP3/Transcription factor staining buffer set (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Also added to the culture with the CTLs was anti-CD107a-PE (1:20, BD, or 1:200, Biolegend), GolgiStop (1:800 ...
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bioRxiv - Biophysics 2020Quote: ... The membrane was probed with an anti-Aβ antibody targeted to residues 1-16 (1:1000, #E 610, Biolegend) and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stained with MHC-I (H-2Kd/Dd-Alexa-647, BioLegend, clone 34-1-25, 1:200 dilution) or MHC-II (I-A/I-E-APC-Cy7 ...
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bioRxiv - Bioengineering 2022Quote: ... Two different tissue sections were immunostained using conjugated antibodies as follows: 1) F4/80-FITC (1:100; BioLegend; 123107), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2022Quote: ... and extracellular antigens (1:200 CD3 BV650, clone 145-2c11, BD 564378; 1:200 NK1.1 PerCP-Cy5.5, clone PK136, BioLegend 10727) for 30 mins in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in FACS buffer containing phycoerythrin (PE)-conjugated PSGL-1 antibody (clone KPL-1, cat. no. 328805, Biolegend). After incubation on ice for 45-60 min ...
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bioRxiv - Immunology 2023Quote: ... sections were blocked with PBS containing 1 % bovine serum albumin (BSA) and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... BV650 anti-CD4 (clone RM4-5, 1:600), AlexaFluor700 anti-CD8 (clone 53-6.7, 1:800) were purchased from BioLegend. e506 Fixable Viability Dye (1:1000) ...
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bioRxiv - Immunology 2023Quote: ... Roughly 0.5 x 106 cells were washed in FACS buffer (PBS + 3% FCS + 0.1% sodium azide) and stained with either FITC-coupled anti-FcεR1 (1:100, clone MAR-1, BioLegend, #134306) and PE-coupled anti-CD117 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... and around 1×106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 h at RT and stained with two staining panels: (1) anti-CD3 (clone UCHT1; #300415, BioLegend, USA) and anti-CD14 (clone HCD14 ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-beta III Tubulin (mouse, 1:1000, Abcam, Cat. No: ab78078 or mouse, 1:400, BioLegend, Cat. No: 801201). The following day ...
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bioRxiv - Cell Biology 2023Quote: ... an RBD - HC2.RBD.rFC (Metafora – 1:5) was used in conjunction with Alexa 647 donkey anti rabbit Poly4064 (1:50 Biolegend).
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bioRxiv - Neuroscience 2023Quote: ... Cells were labelled with rat-anti-Prominin-1 antibody (Brilliant Violet 421-conjugated; BioLegend, #141213, Lot# B255870, 1:1000) and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used were as follows: anti-βIII tubulin (rabbit, 1:5000, BioLegend, 801201; mouse, 1:3000, BioLegend, 801213), anti-MBP (mouse ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, # 423107)) and incubated on ice for 30 min ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and incubated with fluorescently labeled antibodies (CD4 PE, clone RM4-5, 1:400, BioLegend; CD8a FITC, clone 53-6.7, 1:400, BioLegend; B220 PE-Cy7 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Triton X-100) (primary antibodies: Rabbit s100b, abcam, AB52642-1001, 1:500, Mouse 6e10 Biolegend 803015, 1:500). Next day sections were washed 3×5 minutes with PBS and incubated with secondary antibodies for 2 hours at room temperature diluted in antibody buffer (Goat-anti-Rabbit Alexa 488 ThermoFisher A11034 ...
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bioRxiv - Immunology 2024Quote: Immune cells were isolated as described above then stained with the following antibodies (Live Dead AF350 NHS Ester, Thermofisher, #A10168, 1:400; CD62L-BUV737, BD Bioscience, #612833, 1:100; NK1.1-BV711, Biolegend, #108745 ...
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bioRxiv - Biochemistry 2024Quote: ... BD Biosciences (human CD45-PE, clone HI30, 1:200, mouse CD45-APC-Cy7, clone 30-F11, 1:100, BioLegend). Upon confirmation of engraftment ...
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bioRxiv - Genomics 2024Quote: ... DMEM containing 0.2% BSA and 1mg/ml DNase stained with 1:10,000 DAPI and 1:1000 Draq5 (BioLegend, 424101), and live cells were sorted ...
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bioRxiv - Cancer Biology 2024Quote: ... 100k T-cells were mixed with 50k A375 or A375-STn cells (E:T ratio 1:1) in complete medium and 5 ug/ml brefeldin A (Biolegend). Co-cultures were incubated for 3.5 hours in a cell culture incubator ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were permeabilized and blocked with 0.1% Triton X-100 in PBS supplemented with 10% normal goat serum for 1 hour at room temperature and incubated with mouse anti-Tuj1 (1:300; BioLegend) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... A cocktail of Abs for surface markers at the predetermined concentrations (CD31-APC 1:100, Biolegend; CD45-APC 1:200, Biolegend, CD117-APC 1:100 ...
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bioRxiv - Immunology 2021Quote: ... at 2:1 ratio for 1 h at 4C to ensure maximum staining quality before surface staining with CD20-FITC (2H7, Biolegend) for an additional 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... DC purity was assessed by antibodies against CD141 (1:10, APC, AD5-14H12, 130-113-314, Miltenyi) and XCR1 (1:10, PE, S15046E, 372604, BioLegend), and T cell phenotype was assessed by antibodies against CD25 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: Free floating 30 μm sagittal brain sections were treated for antigen retrieval using sodium citrate buffer and subsequently stained using primary antibodies for Iba1 (1:250, rabbit, Wako) and anti-6E10 (1:1000, Biolegend), and the corresponding secondary antibodies and nuclear counterstain using DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... All FACS antibodies were used at 1:100 dilution in the presence of TruStain fcX blocker (101320, Biolegend, 1:50). For PDGFRa immunofluorescence staining ...
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bioRxiv - Cancer Biology 2020Quote: ... and 200 μl of primary antibody (1:200 anti-αSMA antibody, eBioscience, clone 1A4, efluor 570; 1:200 anti-CD31 antibody, Biolegend, clone MEC13.3 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% Sodium Azide and with one of the following primary antibody dilutions: Mouse Anti-HA (1:2000; HA.11, Biolegend), Mouse Anti-V5 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then blocked (PBS-0.25% Triton X-100+ 1% BSA (bovine serum albumin)) for 1h at RT and then primary antibodies (anti-βIII tubulin (Tuj1, 1:1000, BioLegend), anti-NFH (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Stained cells and “fluorescence minus one” controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Biolegend, #424001) as a live/dead stain ...
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bioRxiv - Immunology 2022Quote: ... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U/ml erythropoietin) for 1 hour following disaggregation before being stained (anti-CD71 Brilliant Violet 421, 1:5000 dilution – 113813 Biolegend, RRID ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with K562 at a 1:1 E/T ratio with addition of a CD107a FITC mAb (H4A3, BioLegend) in a 96-round bottom plate with a centrifugation step of 5min at 500rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of protein (1/10 of input transferred in a new tube) was incubated together with 1 μL of anti-HA antibodies (BioLegend 901502 anti-HA.11 clone 16B12 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5mM EDTA and 1% BSA) and incubated with the following primary antibodies: anti-mouse CD45 (Brilliant Violet 570, 30-F11, 1:200, Biolegend), anti-mouse Fce-R1 alpha (PE-Cyanine7 ...
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bioRxiv - Immunology 2020Quote: ... and other epithelial cells markers (anti-mouse CD326 Brilliant Violet 605, 1:200 Biolegend 118227 and PerCP/Cy5.5 anti-mouse CD24, 1:200 Biolegend 101824) for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... for sorting or CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-pacific blue (Biolegend, clone GoH3, 1:200) for analysis ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with the tetramer plus CD3-eFluor 450 (1/100, 48-0032-82 eBioscience) and CD8-FITC (1/100, 300905 BioLegend) at 4°C for 30 min ...