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Citations for BioLegend :
3001 - 3050 of 4492 citations for 2 4 6 Trichlorophenol 13C6 99% 100 Ug Ml In Isooctane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of antibodies for 30-minutes on ice: VEGFR2-PECy7 (Biolegend, #136414, 1:100), CD45-APC (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and the blood was further diluted at 1:100 ratio in the cell staining buffer (Biolegend, 420201). The cells were stained with CD45 flow antibody and proceeded for flow cytometry to analyze the frequency of CD45+ immune cells.
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bioRxiv - Genomics 2022Quote: ... and PE anti-DYKDDDDK Tag antibody (0.6 ul antibody per million cells in 100 ul) (Biolegend #637310) for DYKDDDDK-tagged Cas9 was carried out at room temperature for 45 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged and 500xg for 5 min and blocked with Fc block CD16/32 (Biolegend, #101302, 1:100), for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... and cells were stained with rat anti-mouse ICAM-1 (1:100; Biolegend YN1/1.7.4 in PerCP), rat anti-mouse CD45 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... Freshly isolated cells were pre-incubated with anti-CD56 Alexa Fluor 647 (clone HCD56, Biolegend, 1:100) for 20 minutes in a conical tube then incubated on #1.5 coverslips that had been pre-coated with poly-L-lysine for an additional 20 minutes at 37°C 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Substrate conversion was stopped after 20 minutes with 100 μL stop solution (2N H2SO4) purchased from BioLegend. Plates were washed with PBS plus 0.05% Tween20 in-between incubations ...
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bioRxiv - Immunology 2020Quote: ... Neutrophil presence in the kidney was confirmed by staining with anti-mouse Ly6G Al647 (1:100, Biolegend). Tissues were mounted using Prolong Gold with DAPI mounting medium (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and 250,000 cells were resuspended in 100 µl of FACS buffer containing TruStain FcX blocker (Biolegend 422302) and MojoSort CD45 Nanobeads (Biolegend 480030) ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used: Brilliant Violet 421 anti-mouse IgD Antibody (BioLegend 405725, 1:100 dilution), Brilliant Violet 421 anti-mouse IgM Antibody (BioLegend 406517 ...
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bioRxiv - Microbiology 2022Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...
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bioRxiv - Pathology 2024Quote: ... and the cell pellet was stained with anti-Cd31 (Alexa Fluor 488-conjugated 1:100, Biolegend, 102514), anti- Cd45 (Alexa Fluor 488-conjugated 1:100 ...
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bioRxiv - Genomics 2023Quote: ... biotin conjugated lineage antibodies on ice for 30 min: CD3 clone C145-2c11 (Biolegend, 1000304; 1:100), CD4 clone GK15 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 1 × 106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... ST2 and CD13 was verified using FITC-coupled anti-FcεRI (1:100, clone MAR-1, BioLegend, #134306), PE-coupled anti-KIT (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... the following primary antibodies and secondary antibodies were used: mouse anti-HA antibody (1:100) (16B12, BioLegend), rabbit anti-HA antibody (1:200 ...
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bioRxiv - Immunology 2024Quote: ... before staining with anti-mCD3-PB and anti-m/hB220-AF488 (Biolegend, #100214 and #103225, 1:100). After washing ...
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bioRxiv - Immunology 2024Quote: ... and MHC Class II (MHCII) I-A/I-E (stains MMs, 1:100, clone MS/114.15.2, Biolegend) were used ...
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bioRxiv - Cancer Biology 2024Quote: Cells were blocked with TruStain fcX™ (anti-mouse CD16/32, BioLegend, #101319, clone 93, 1:100) for 10 minutes on ice ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed and blocked hMPCs were incubated overnight at 4°C with an anti-BrdU antibody (1:400 dilution, Biolegend) followed by PBS washes and a 60 min incubation with an Alexa-Fluor 488 conjugated anti-mouse secondary antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse interleukin (IL)-4 PE (504104) and anti-mouse IL-17 PE (517008) (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Samples were next stained for surface markers for 20min at 4°C with: PerCP-Cy5.5-conjugated anti-TCRβ (H57–597, BioLegend), APC-CY7-conjugated anti-B220 (RA3-6B2 ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Immunology 2019Quote: ... Depletion was confirmed by analysis of dLN and skin T cells stained with anti-CD4 clone RM4-4 (Biolegend), which binds a nonoverlapping epitope.
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBS-Tx (1:1,000 for mouse anti-HA, #901514 BioLegend; 1:1,000 for rabbit anti-TOM20 ...
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bioRxiv - Cell Biology 2020Quote: After coating with poly-L-Lysine, fluorodishes were coated overnight at 4 °C with αCD3ε (OKT3, #16-0037-85, eBioscience) + αCD28 (CD28.2, #302914 Biolegend), washed three times and pre-warmed at 37 °C for 10–15 min ...
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bioRxiv - Immunology 2022Quote: ... and stained for 30 min at 4°C with a fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 Ab (BioLegend). The cells were then fixed ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Immunology 2020Quote: ... then stained 15 minutes for surface markers and fixed for 20 minutes with 4% paraformaldehyde (PFA) in PBS (BioLegend). Cells were then permeabilized with 0.5 % saponin in Running buffer and stained for intracellular markers ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μL of WCE was incubated overnight at 4°C with 10μg of monoclonal RNA Poll antibody (Biolegend, USA), 2μg of anti-myc antibody (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Immunology 2020Quote: ... Sections were stained at 4°C on an agitation stage overnight with anti-CD169 and anti-Ly6G antibodies (BioLegend). Stained sections were microscopically analyzed using Leica SP8 confocal microscope (Leica Microsystem ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...
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bioRxiv - Immunology 2024Quote: ... Stained spleen or LN samples were fixed with 1-4% phosphate-buffered paraformaldehyde solution (FluoroFix or Fixation Buffer, Biolegend). To detect transcription factors and certain intracellular proteins ...
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bioRxiv - Immunology 2022Quote: Human cells were surface stained for 25 minutes at 4 °C with antibodies to with anti-CD3 (Clone UCHT1, eBioscience) and anti-CD56 (Clone 5.1H11, Biolegend).
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bioRxiv - Immunology 2023Quote: ... cells were stained in the dark for 15 min at 4°C with fluorochrome-conjugated antibodies in PBS containing anti-CD16/CD32 (2.4G2; BioXcell or BioLegend). Cells were washed and ...
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bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
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bioRxiv - Neuroscience 2023Quote: ... and stained for intracellular cytokines (IFN-γ PE #505808, IL-4 BV421 #504120, IL-17A PE-Cy7 #506922, Biolegend) with the Cyto-Fast™ Fix/Perm Buffer Set (#426803 ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were stained 30min at 4°C in the dark with PerCP/Cyanine5.5 anti-mouse Ly6G (clone:1A8, cat. no. 127615, BioLegend), FITC anti-mouse/human CD11b (clone:M1/70 ...
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bioRxiv - Immunology 2023Quote: ... Slides were then blocked and incubated overnight at 4°C with anti-Lysozyme antibody diluted 1:400 (Biolegend, #860001). Slides were washed and incubated with anti-rabbit Alexa Fluor 555 diluted 1:500 (Invitrogen ...
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bioRxiv - Systems Biology 2022Quote: Sorted live single cells were centrifuged at 400 x g for 5min at 4°C and resuspended in 25μL of cell staining buffer (BioLegend). Human TruStain FcX FC blocking reagent (BioLegend ...