Labshake search
Citations for BioLegend :
2951 - 3000 of 3345 citations for Recombinant Human MMP7 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
-
bioRxiv - Immunology 2022Quote: ... and the cells were resuspended in 200 μL of PBS supplemented with 1% FBS and containing 2.5 μL of Alexa Fluor 647-conjugated anti-human CD127antibody (BioLegend) or Alexa Fluor 647-conjugated mouse IgG1 k isotype control (BioLegend) ...
-
bioRxiv - Immunology 2020Quote: ... Antigen experienced CAR T cells were stained for CD69 expression using APC-conjugated anti-human CD69 antibody (Biolegend #310910). Flow cytometric data was acquired using a BD LSRFortessa with BD FACSDiva software ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
-
bioRxiv - Genomics 2019Quote: ... CD4+ T helper cells were sorted as naive T cells (CD4+CD25−CD45RA+) or memory T cells (CD4+CD25−CD45RA−) using the following antibodies: anti-human CD45RA-PERCPCy5.5 (Clone HI100, Biolegend), anti-human CD4-APC-Cy7 (Clone OKT4 ...
-
bioRxiv - Genomics 2019Quote: ... Cells were labelled for CD47 expression with biotin anti-human CD47 antibody (clone CC2C6, BioLegend, Cat.# 323104, RRID: AB_756134) at 0.0625 µg/100 µl ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was then blocked with 10% normal donkey serum/0.1% Triton X- 100/0.01% NaN3 followed by staining with Alexa Fluor 488 conjugated anti-human CD4 OKT 4) antibody (Biolegend) at 4° Celsius overnight (1:200 diluted in PBS) ...
-
bioRxiv - Bioengineering 2021Quote: ... Enriched EPCs from Day 10 were examined by staining with APC Anti-Human CD71 antibody (334107; Biolegend; 1:100) and incubating for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... and DAPI/conjugated PECAM1 antibody (0.04 µg/ml DAPI, Sigma, 10236276001; 1:250 Alexa647-anti-human PECAM1, Biolegend, 303112) in blocking buffer for 1 hr each ...
-
bioRxiv - Cell Biology 2021Quote: ... samples were washed with FACS buffer (0.3% BSA in PBS) and stained with Human TruStain FcX (BioLegend, Cat# 422301) for 15 minutes ...
-
bioRxiv - Immunology 2021Quote: ... Antibodies used to stain for IL-4Rα were APC anti-human CD124 (IL-4Rα) (Biolegend, clone G077F6, 1:100) and Live/Dead Fixable Green (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... The following antibodies were used for intracellular staining: Anti-human TBET FITC (1:50, clone 4B10, BioLegend, cat # 644812), Anti-human Granzyme B FITC (1:100 ...
-
bioRxiv - Microbiology 2021Quote: ... Alexa Fluor 647-labelled monoclonal mouse anti-human CD62P (P-Selectin) antibody (Clone AK4, BioLegend, San Diego, CA, USA), Alexa Fluor 647-labelled goat anti-mouse IgG (GAMIG AF-647 ...
-
bioRxiv - Immunology 2020Quote: ... and Il6) in treated PBMC supernatants were quantified by ELISA using the ELISA MAX Deluxe Set Human CCL2 (Biolegend), ELISA MAX Deluxe Set Human IL8 (Biolegend) ...
-
bioRxiv - Immunology 2020Quote: ... Bacteria were opsonised in heat-inactivated human serum for 30 minutes at 37°C and labelled via incubation with 10μM carboxyfluorescein succinimidyl ester (CFSE) (Biolegend) for 30 minutes at 37°C ...
-
bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
-
bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako; and FITC-Annexin V from Biolegend) for 20 min at RT and centrifuged on 45° fixed angle rotor FA-45-30-11 (Eppendorf ...
-
bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
-
bioRxiv - Genetics 2021Quote: To study the human HSPC phenotype of the gene edited cells were stained with hCD34-Pecy7 (581 clone, Biolegend), hCD38-PE (HB7 clone ...
-
bioRxiv - Bioengineering 2022Quote: ... the streptavidin modified nanovial solution was then mixed with a solution containing 8 μg/mL anti-human CD45 (Biolegend) at equal volume ...
-
bioRxiv - Microbiology 2022Quote: Cytokine concentration of the basolateral compartments were measured using the human antivirus response panel (13-plex) kit (LEGENDplex BioLegend). The kit was used according to the manufacturer’s manual ...
-
bioRxiv - Immunology 2022Quote: ... Cells were spun down and supernatant was frozen for Legendplex analysis (LEGENDplex Human CD8/NK Panel 13-plex, BioLegend, according to the manufacturer’s information) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and genotyped for HLA*A:02 via flow cytometry with purified anti-human HLA-A2 antibody clone BB7.2 (BioLegend). HLA-A*02 positive samples were selected and dendritic cells were generated via plate adherence and pulsed with mHAs that are not endogenous to the sample (Peptide 2.0 Inc) ...
-
bioRxiv - Microbiology 2024Quote: ... and IL-1β concentrations were determined with the ELISA MAX™ Deluxe Set Human IL-1β (BioLegend, Cat# 437004) in 96-well half area Costar assay plates (Corning ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 100,000 IFNγ-treated tumor cells were seeded together with 200,000 pTc or TiTc per well in Tc medium containing anti-human CD107a-FITC (Biolegend). After 1h incubation ...
-
bioRxiv - Cancer Biology 2024Quote: ... IFN-γ secretion to validate CAR-T cell activation was measured using the Human INF-γ ELISA kit (Biolegend) or Human IFN-γ DuoSet ELISA kit (R&D Systems) ...
-
bioRxiv - Cancer Biology 2024Quote: ... T cells were activated for 3 days using 3 μg anti-human CD3 and CD28 antibodies (Biolegend, 300438, 377604) and 50 ng/mL of IL-2 (Peprotech ...
-
bioRxiv - Molecular Biology 2024Quote: ... the cells were stained with live dye (Calcein AM) and PE anti-human CD45 antibody (BioLegend, cat no 368510) mixture ...
-
bioRxiv - Immunology 2024Quote: ... Human CD4+ T cells (4X105/well in 96-well plate) were activated with plate-coated αCD3 (5µg/ml, Biolegend) and αCD28 (10µg/ml ...
-
bioRxiv - Immunology 2024Quote: ... These fixed and permeabilized cells were further stained with mouse anti-human IFN-γ antibody conjugated with PE (BioLegend) and Brilliant Violet 421 conjugated mouse anti-human TNF antibody (BioLegend) ...
-
bioRxiv - Immunology 2023Quote: ... aureus Newman (from 2.106 to 2.104/well) in the presence of anti-human CD107a-AF647 mAb (clone H4B4, Biolegend, France) added at the beginning of stimulation in serum and antibiotic free RPMI media in 96 well round bottom plates ...
-
bioRxiv - Immunology 2023Quote: ... Each B cell sample was split into two aliquots of at least 500,000 cells for labeling with different TotalSeqC anti-human cell hashing antibodies (BioLegend). Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...
-
bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human TCR α/β antibody (IP26: Biolegend), HLA.A2/9V-AF647 or CD19-AF647 to determine the concentration of the staining agent leading to label saturation for CMV-specific RA14 TCR T-cells ...
-
bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Live naïve CD4+ T cells were purified from PBMCs post staining with fluorescence labelled antibodies to human CD3(OKT3, Biolegend), CD4 (RPA-T4 ...
-
bioRxiv - Cancer Biology 2023Quote: ... were coated with 50 µL per well of Purified anti-human IFNγ Antibody (clone MD-1; #507502, BioLegend, USA) diluted 1:200 in 1X PBS (#5460-0023 ...
-
bioRxiv - Neuroscience 2023Quote: ... 1-16 Antibody (1:000, Clone 6E10, N-terminal, a.a. 1-16, human specific only, 803014 BioLegend, CA, USA); β-Amyloid ...
-
bioRxiv - Neuroscience 2023Quote: ... 17-24 Antibody (1:1000, Clone 4G8, N-terminal, a.a. 17-24, human & mouse specific, 800708 BioLegend, CA, USA); Amyloid Precursor Protein (1:1000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Human IL-2 ELISA MAX Deluxe kit and Human IL-12 (p70) ELISA MAX Deluxe kit in accordance with the manufacturer’s instructions (BioLegend).
-
bioRxiv - Immunology 2023Quote: ... BSA/0.01% (v/v) Tween-20 in cold RNase-free PBS + 10 μl Human TruStain FcX blocking solution (#422301, BioLegend)) and incubated for 10 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
-
bioRxiv - Bioengineering 2023Quote: ... For surface antigen staining 20 min incubation with the Alexa Fluor 488 anti-human CD29 (Biolegend, clone TS2/16), PE anti-human CD184[CXCR4] (Biolegend ...
-
bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
-
bioRxiv - Cell Biology 2022Quote: ... Then cells were resuspended in Maxpar® cell staining buffer (Fluidigm) and blocked with Human TruStain FcX™(Biolegend) for 10 minutes at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
-
bioRxiv - Immunology 2023Quote: ... each sample was labelled with 0.1 ug of a cell hashing antibody (TotalSeq-B anti-human Hashtag antibodies 1 through 8, BioLegend). Cells were labelled for 30 minutes on ice in the dark ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and incubated overnight at 4 °C on a tube rotator at 18 rpm in 800 μL of PBS/0.2% BSA containing 15 nM human IgG1 (BioLegend) that had been conjugated with Alexa647 using NHS chemistry (Molecular Probes ...
-
bioRxiv - Neuroscience 2023Quote: ... single cell suspensions were incubated for 15 minutes at room temperature in the dark with Human TruStain FcX (Biolegend) at a concentration of 5µL per million cells to block unspecific Fc Receptor binding ...
-
bioRxiv - Immunology 2024Quote: Up to 20,000 cells per sample (Table S1) were stained with the TotalSeq-C Human Universal Cocktail v1 (BioLegend) as per manufacturer’s instructions ...