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2951 - 3000 of 4343 citations for FSTL1 Mouse HEK 293 His since 2019

• Dehydrozaluzanin C- derivative protects septic mice by alleviating over-activated inflammatory response and promoting the phagocytosis of macrophages

  Ying-xiang Zou, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... and Brilliant Violet 421™ anti-mouse I-A/I-E (Biolegend, 107631, USA). And then cells were analyzed by Flow cytometry (Beckman ...
• Methylmalonic acid induces metabolic abnormalities and exhaustion in CD8⁺ T cells to suppress anti-tumor immunity

  Joanne D. Tejero, et al., bioRxiv - Immunology 2024

  Quote: ... that were coated overnight with 10 μg/mL of anti-mouse CD3ε antibody (Biolegend) and washed twice with PBS prior to seeding ...
• SARS-CoV-2 modulates NK cell responses via induction of HLA-E and triggers expansion of adaptive NK cells during acute infection

  Mohammad Zahidul Hasan, et al., bioRxiv - Immunology 2024

  Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
• Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica

  Han Jiang, Herbert J. Santos, Tomoyoshi Nozaki, bioRxiv - Cell Biology 2024

  Quote: ... The anti-HA 16B12 mouse monoclonal antibody was purchased from Biolegend (San Diego, USA). Lipofectamine ...
• Establishing a method for the cryopreservation of viable peripheral blood mononuclear cells in the International Space Station

  Hiroto Ishii, et al., bioRxiv - Cell Biology 2024

  Quote: Antibodies and reagents employed included FITC-anti-mouse NK1.1 (BioLegend, clone PK136, Cat#108705), PerCP-eFluor710-anti-mouse CD172a (eBioscience ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... under Th1-polarizing condition (10 ng/mL recombinant mouse IL-12 (p70) (#577004, BioLegend), and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11 ...
• Retinal microglia exacerbate uveitis by functioning as local antigen-presenting cells

  Shintaro Shirahama, et al., bioRxiv - Immunology 2024

  Quote: ... Brilliant Violet 785 anti-mouse CD45 (30-F11) (1:50) (BioLegend, San Diego, CA), PE anti-mouse I-A/I-E (M5/114.15.2 ...
• IFNγ-dependent remodelling of the myeloid landscape underlies control of IFNγ-insensitive tumours

  Vivian W.C. Lau, et al., bioRxiv - Immunology 2024

  Quote: ... For LEGENDplex™ Mouse Cytokine Release Syndrome Panel (13-plex) assays (Biolegend, Cat. 741024), 25uL of supernatant was used following manufacturer’s protocols and samples were analysed by BD LSRFortessa.
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... Antibodies used for flow cytometry are: BV650 rat anti-mouse CD45 (Biolegend Cat# 103151), BUV395 Rat anti-mouse CD11b (BD Horizon Cat# 563553) ...
• Characterization and functional analysis of Toxoplasma Golgi-associated proteins identified by proximity labelling

  Rebecca R. Pasquarelli, et al., bioRxiv - Microbiology 2024

  Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (BioLegend; 901515). The V5 epitope was detected with mouse mAb anti-V5 (Invitrogen ...
• A T6SS in the coral pathogen Vibrio coralliilyticus secretes an arsenal of anti-eukaryotic effectors and contributes to virulence

  Shir Mass, et al., bioRxiv - Microbiology 2024

  Quote: ... coli RNA polymerase sigma 70 (mouse mAb #663205; BioLegend; referred to as α-RNAP) antibodies at a dilution of 1:1000 ...
• The ICF2 gene Zbtb24 specifically regulates the differentiation of B1 cells via promoting heme synthesis

  Jun Wang, et al., bioRxiv - Immunology 2024

  Quote: CD4+ T cells were isolated with MojoSortTM Mouse CD4 T cell isolation kit (Biolegend) from spleens of WT mice with > 90% purity ...
• Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response

  Brandon H. Hayes, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
• Suppression of epithelial proliferation and tumorigenesis by immunoglobulin A

  Gregory P. Donaldson, et al., bioRxiv - Immunology 2023

  Quote: ... including a counterstain with 1:200 AF488 anti-mouse CD326 (EPCAM) (Biolegend Cat#118210). Stained sections were mounted with Fluoromount G (Southern Biotech ...
• Activation of the aryl hydrocarbon receptor inhibits neuropilin-1 upregulation on IL-2 responding CD4⁺ T cells

  Simone Sandoval, et al., bioRxiv - Immunology 2023

  Quote: ... H-2Dd (Anti-mouse H-2Dd antibody; clone 34-2-12; Biolegend; Cat #110606), CD25 (BV510 Rat Anti-Mouse CD25 ...
• Haptenic adducts of β-lactam antibiotics elicit antibody responses with narrow clonality and specificity

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... Surface staining was performed using anti-mouse F4/80-PE (1:200, BM8, Biolegend), anti-mouse Gr-1 (1:200 ...
• IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells

  Victoria A. Wong, et al., bioRxiv - Immunology 2023

  Quote: ... and rat anti-mouse IL-2Rα (clone PC61) were from Biolegend (San Diego, CA). Purified mouse IL-2Rα was from SinoBiological (Wayne ...
• CD155 blockade enhances allogeneic natural killer cell-mediated antitumor response against osteosarcoma

  Monica M Cho, et al., bioRxiv - Cancer Biology 2024

  Quote: ... purified rat anti-mouse anti-DNAM-1 (Biolegend Cat. No. 128822, San Diego, CA), or purified rat anti-mouse anti-NKG2D (Biolegend Cat ...
• Differential dysregulation of β-TrCP1 and -2 by HIV-1 Vpu leads to inhibition of canonical and non-canonical NF-κB pathways in infected cells

  Suzanne Pickering, et al., bioRxiv - Microbiology 2022

  Quote: ... Lysates were incubated with mouse anti-HA antibody (anti-HA.11 clone 16B12, BioLegend) or rabbit anti-Flag antibody (F7425 ...
• Hormonal gatekeeping via the blood brain barrier governs behavior

  Linyang Ju, et al., bioRxiv - Neuroscience 2022

  Quote: ... Antibodies used were as follows: 1:1000 mouse anti-HA.11 epitope (16B12, BioLegend); 1:2000 mouse anti-β-tubulin (E7 ...
• Elucidation of Amyloid-Beta’s Gambit in Oligomerization: Truncated Aβ fragments of residues Aβ1-23, Aβ1-24 and Aβ1-25 rapidly seed to form SDS-stable, LMW Aβ oligomers that impair synaptic plasticity

  Beatriz Gil, et al., bioRxiv - Neuroscience 2022

  Quote: ... Detection of Aβ species was achieved using the mouse monoclonal HRP-conjugated 6E10 (Biolegend) antibody at a dilution of 1:1000 ...
• ERBB-activated myofibroblastic cancer-associated fibroblasts promote local metastasis of pancreatic cancer

  Gianluca Mucciolo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... cells were stained for 30 minutes with anti-mouse CD45-PerCP/Cy5.5 (103132; BioLegend), TCR-β-Alexa488 (109215 ...
• ERBB-activated myofibroblastic cancer-associated fibroblasts promote local metastasis of pancreatic cancer

  Gianluca Mucciolo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... cells were stained for 30 minutes with anti-mouse CD31-PE/Cy7 (102418; BioLegend), CD45-PerCP/Cy5.5 (103132 ...
• Clec12a tempers inflammation while restricting expansion of a colitogenic commensal

  Tyson R. Chiaro, et al., bioRxiv - Immunology 2023

  Quote: ... Cytokines were analyzed with LEGENDplex™ Mouse Inflammation Panel (13-plex) (Biolegend, cat# 740150). Data was analyzed using the LEGENDplex™ data analysis software.
• Transient EZH2 suppression by Tazemetostat during in vitro expansion maintains T cell stemness and improves adoptive T cell therapy

  Yingqin Hou, et al., bioRxiv - Immunology 2023

  Quote: OT-I T cells were restimulated with anti-mouse CD3 antibody (Clone: 17A2, Biolegend, Cat#100238 ...
• Interferon-induced lysosomal membrane permeabilization and death cause cDC1-deserts in tumors

  E. Aerakis, et al., bioRxiv - Immunology 2023

  Quote: MutuDC1 cells were treated with mouse recombinant 20ng/ml IFN-β1 (Biolegend Cat.581302), mouse 80ng/ml IFN-α (Miltenyi Cat ...
• Olfactory and neuropeptide inputs to appetite neurons in the arcuate nucleus

  Donghui Kuang, et al., bioRxiv - Neuroscience 2023

  Quote: ... brain sections were incubated with biotinylated mouse anti-HA antibodies (BioLegend, #901505, 1:300) at 4°C overnight or at 37°C for an hour ...
• Unconventional Activation of IRE1 Enhances TH17 Responses and Promotes Neutrophilic Airway Inflammation

  Dandan Wu, et al., bioRxiv - Immunology 2023

  Quote: ... IL-22 was detected by using a Mouse IL-22 ELISA Kit (B284521, Biolegend) following the manufacturer’s instruction.
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV650 Rat Anti-Mouse CD45R/B220 (Clone RA3-6B2; BioLegend; Cat#103241; RRID: AB_11204069) BV421™ anti-mouse/human CD45R/B220 Antibody (Clone RA3-6B2 ...
• Compartment specific responses to contractility in the small intestinal epithelium

  Taylor Hinnant, Wenxiu Ning, Terry Lechler, bioRxiv - Cell Biology 2023

  Quote: ... Rabbit anti-mouse non muscle myosin heavy chain IIC (Biolegend, Cat.#: 919201; 1:100), Rat anti-mouse Ki67 (Invitrogen ...
• ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion

  Demis Menolfi, et al., bioRxiv - Genetics 2023

  Quote: ... with each antibody used at 1:200 dilution: FITC anti-mouse CD43 (Biolegend, 553270); PE anti-mouse IgM (Southern Biotech ...
• Noncanonical TRAIL Signaling Promotes Myeloid-Derived Suppressor Cell Abundance and Tumor Progression in Cholangiocarcinoma

  Emilien Loeuillard, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cells were stained with TotalSeq™-C Mouse Universal Cocktail (BioLegend® V1.0, 199903). Finally ...
• Microglial cannabinoid receptor type 1 mediates social memory deficits produced by adolescent THC exposure and 16p11.2 duplication

  Yuto Hasegawa, et al., bioRxiv - Neuroscience 2023

  Quote: ... PE rat anti-mouse CD11b (cat # 101208, BioLegend, clone M1/70, 2 ng/μl), APC rat anti-mouse ACSA-2 (cat # 130-117-535 ...
• Data-driven discovery of innate immunomodulators via machine learning-guided high throughput screening

  Yifeng Tang, et al., bioRxiv - Immunology 2023

  Quote: ... Recombinant Mouse GM-CSF (carrier-free; 20 ng/ml; BioLegend, San Diego, California, USA), 2 mM L-glutamine (Life Technologies) ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... Alexa Fluor 700 anti-mouse/human CD45R/B220 antibody(1:50; Biolegend, RA3-6B2), BUV395 anti-mouse CD11b(1:200 ...
• Vascular Endothelial-derived SPARCL1 Exacerbates Viral Pneumonia Through Pro-Inflammatory Macrophage Activation

  Gan Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... Brilliant Violet 711™ anti-mouse/human CD11b antibody (1:200; Biolegend, M1/70), Brilliant Violet 421™ anti-mouse F4/80 antibody(1:100 ...
• Elevated CD153 Expression on Aged T Follicular Helper Cells is Vital for B cell Responses

  Alyssa L. Thomas, et al., bioRxiv - Immunology 2023

  Quote: ... in the presence or absence of 20ng/ml of recombinant mouse IL-6 (Biolegend) and/or 200ng/ml of nivalenol (Cayman Chemical ...
• RIPK3 promotes brain region-specific interferon signaling and restriction of tick-borne flavivirus infection

  Marissa Lindman, et al., bioRxiv - Immunology 2023

  Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend ...
• Loss of HSC stemness identity is associated with exhaustion and hyporesponsiveness in GATA2 deficiency syndrome

  Laetitia Largeaud, et al., bioRxiv - Cell Biology 2023

  Quote: ... incubated with a fluorescently labelled antibody Endomucin (Rat anti-mouse, clone TC15-12F12.2, BioLegend) at 4°C overnight under agitation ...
• The Toxoplasma subpellicular network is highly interconnected and defines parasite shape for efficient motility and replication

  Peter S. Back, et al., bioRxiv - Microbiology 2023

  Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (BioLegend; 901515) or rabbit polyclonal antibody (pAb ...
• Zfp318 controls the recallability of memory B cells

  Yifeng Wang, et al., bioRxiv - Immunology 2023

  Quote: ... the plate was further incubated with HRP-conjugated goat anti-mouse IgG (poly4053, Biolegend) at 1:20,000 for 1 h before development with TMB Substrate Set (421101 ...
• Regulation of BCR-mediated Ca²⁺ mobilization by MIZ1-TIMBIM4 safeguards IgG1⁺ GC B cell positive selection

  Lingling Zhang, et al., bioRxiv - Immunology 2023

  Quote: ... BV421 anti-mouse IgD Antibody (Clone 11-26c.2a; BioLegend; Cat#405725; RRID: AB_2562743) BUV395 Rat Anti-Mouse IgD (Clone 11-26c.2a ...
• A new type of transcriptional reprogramming by an IRF4 mutation in lymphoma

  Nikolai Schleussner, et al., bioRxiv - Cancer Biology 2022

  Quote: ... blocked with TruStain FcX (α-mouse CD16/32; 10 min, 4°C; #101320, BioLegend) and stained (20 min ...
• Lipid Biosynthesis Perturbation Impairs Endoplasmic Reticulum-Associated Degradation

  Samantha M. Turk, et al., bioRxiv - Cell Biology 2022

  Quote: ... The following primary antibody dilutions were used: mouse anti-HA.11 (Clone 16B12; BioLegend) at 1:1,000 ...
• Impaired Barrier Integrity of the Skeletal Muscle Vascular Endothelium Drives Progression of Cancer Cachexia

  Young-Mee Kim, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and stained with primary rat anti-mouse CD31 antibody (BioLegend, 102502, 1:1000 dilution) for 18 h at 4 °C ...
• Innate immune receptor C5aR1 regulates cancer cell fate and can be targeted to improve radiotherapy in tumours with immunosuppressive microenvironments

  Callum Beach, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Nonspecific binding was blocked using an anti-mouse CD16/32 (BioLegend, San Diego, CA) antibody ...
• Epithelioids: Self-sustaining 3D epithelial cultures to study long-term processes

  Albert Herms, et al., bioRxiv - Cell Biology 2023

  Quote: ... was added 1:200 and Alexa fluor 647 anti-human/mouse CD49f (Biolegend, 313610) was added 1:250 ...
• Epithelioids: Self-sustaining 3D epithelial cultures to study long-term processes

  Albert Herms, et al., bioRxiv - Cell Biology 2023

  Quote: ... was added 1:200 and Alexa fluor 647 anti-human/mouse CD49f (Biolegend, 313610) was added 1:250 ...
• Mast cell-derived BH4 is a critical mediator of postoperative pain

  Philipp Starkl, et al., bioRxiv - Immunology 2023

  Quote: ... IL-6 was measured using the ELISA MAX Standard Set Mouse IL-6 (Biolegend).
• Humanized CD36 mouse model supports the preclinical evaluation of therapeutic candidates targeting CD36

  Xiulong Xie, et al., bioRxiv - Immunology 2023

  Quote: ... and hCD36 mice (peritoneal exudative macrophages) using Anti-mouse CD36 Antibody-APC (Biolegend, 102611) and Anti-mouse CD36-PE (eBioscienceTM ...