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Citations for BioLegend :
251 - 300 of 2171 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... B cells were stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: Cytokine measurements of serum were performed using LEGENDplex murine Th1/Th2 8 -plex Version 3 (BioLegend) as per kit instructions with data acquired using a Fortessa (BD ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used in this study: Mouse-anti-Tubulin beta-3 (TUBB3) antibody (BioLegend), chicken-anti-MAP2 antibody (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... and supplemented with murine SCF (10 ng/ml) and IL-3 (10 ng/ml) (Peprotech or BioLegend). The cells were serially passaged for ∼1 month ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with Alexa Fluor 647-anti-DYKDDDDK (BioLegend) at 0.5 μg/mL or with Alexa Fluor 488-streptavidin (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with secondary antibody diluted in 3% BSA (Alexa Fluor 594 Goat anti-Rat IgG, BioLegend, #405422) in the dark for 1 hr at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... and neurons were labelled with rabbit anti-tubulin beta 3 (TuJ-1) IgG at 1:500 (Biolegend), in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... one million cells were harvested 3 days after transduction and washed twice with cell staining buffer (Biolegend). Cells were resuspended in 100μL of Cell Staining Buffer (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... we used Ultra-LEAF™ purified anti-human TIM-3 antibody (10μg/ml, clone F38-2E2, Biolegend) or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were allowed to proliferate for 3 days before collecting and staining with CD3e-PE (BioLegend 100308) and CD11b- PECy7 (BioLegend 101216 ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with purified antibody (3 µg/ml) overnight at 4°C (BioLegend, catalog 519202). The next day ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with PBS and coated for >3 h with 1 µg/mL αCD3 (HIT3a, Biolegend) at 37°C ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Molecular Biology 2020Quote: ... cell-impermeant dye 7-amino-actinomycin D (7AAD, BioLegend), cells grown in 6-wells plates were collected upon trypsinization ...
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bioRxiv - Immunology 2023Quote: ... The 7-amino-actinomycin D Viability Staining Solution (BioLegend) was added to eliminate dead cells ...
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bioRxiv - Immunology 2023Quote: ... a 7-amino actinomycin D viability staining solution (BioLegend) was added to each sample to stain dead cells ...
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bioRxiv - Microbiology 2023Quote: ... Seven-amino-actinomycin D (Biolegend, San Diego, CA, USA) was added before analysis with a flow cytometer to exclude nonviable cells ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2021Quote: ... The first surface marker panel included: BV421 and PerCP/Cy5.5 anti-TIM-3 (clones B8.2C12 and RMT3-23, BioLegend), BV510 anti-PD-1 (EH12.1) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 washes were performed prior to secondary antibody staining with anti-rabbit bv421 1:500 (BioLegend 406410) with 3 additional washes after secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... 3-4 × 106 splenocytes after cell surface staining were fixed with Cyto-Fast Fix/Perm buffer (BioLegend, USA) for 20 min at room temperature and washed twice with FACS staining buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... and stained for the intracellular accumulation of IFNγ and TNFα (Supp. Table 3) in 1x Permeabilization Buffer (Biolegend). Samples were analyzed using a Fortessa LSR flow cytometer (BD ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were again washed 3 times before adding 50 µL per well of HRP Streptavidin (#405210, BioLegend, USA) diluted 1:2000 in 1% SureBlock solution and incubated 1 h at RT ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... medium was changed on day 7 to fresh APEL medium supplemented with IL-3 (50 ng/ml) (BioLegend) and G-CSF (10 ng/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST ...
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bioRxiv - Immunology 2024Quote: ... seeded in a F-bottom 96-well plate precoated with 3 µg/mL anti-CD3 (145-2C11; Biolegend) using 5×105 cells (200 µl ...
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bioRxiv - Genomics 2024Quote: ... We cultured BxPC-3 cells in R10 only or in R10 supplemented with 100 IU/mL IFNγ (BioLegend) for 2 days (n = 6 biological replicates per condition) ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with 2 mL SB (350 g for 5 min) and subsequently with 2 mL of 1X Tandem Stabilizer (Biolegend #421802). Cells were resuspended in 200 µL of 1X Tandem Stabilizer.
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines or expanded primary NK cells were stimulated with 3 ug/mL CD28 activating antibody (Biolegend, cat#302933) for 24 hours.
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed in HBSS with 3% BSA and incubated with CD34 Brilliant Violet 421 (BioLegend 230 119321) at 1:400 and CD24 PerCP-Cyanine 5.5 (eBioscience ...
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Immunology 2022Quote: ... added for last 3 hrs of stimulation and stained with IFN-γ (XMG1.2; eBioscience) and IL-17A (TC11-18H10.1; Biolegend) using a Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... mice were injected into the retro-orbital sinus with a total of 3 mg of PE/Cy7-CD45.2 (104, BioLegend) in 200 μl sterile DPBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50,000 CAR-T cells were co-cultured with 100,000 target cells for 5h in the presence of CD107a antibody (Supp. Table 3) and 1x Brefeldin A (Biolegend). Following co-culture ...
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bioRxiv - Immunology 2024Quote: ... intestine and skin (n = 3, PCW 9.5) were stained with the following antibodies (clone, fluorophore, company): CD3 (UCHT1, FITC, Biolegend), CD7 (M-T701 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions from 3 B16-OVA WT tumours were labelled with TotalSeq(TM)-C0301 antibody (Biolegend, Cat. 155861), and 3 IFNγRKO tumours were labelled with TotalSeq(TM)-C0302 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.