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251 - 300 of 2682 citations for TMEM173 Human HEK293 Sumo His since 2019

• Immunogenetic metabolomics revealed key enzymes that modulate CAR-T metabolism and function

  Paul Renauer, et al., bioRxiv - Cancer Biology 2023

  Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
• Modulation of the cAMP levels with a conserved actinobacteria phosphodiesterase enzyme reduces antimicrobial tolerance in mycobacteria

  Michael Thomson, et al., bioRxiv - Microbiology 2020

  Quote: ... incubated for 1 hour with mouse HRP α-His Tag antibody (1:1000 in 5% milk) (BioLegend, USA) and developed ...
• A Phase Separation-Fortified Bi-Specific Adaptor for Conditional Tumor Killing

  Yuyan Liu, Yuting Zhu, Weifan Xu, Pilong Li, bioRxiv - Bioengineering 2023

  Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
• Co-engagement of TIGIT⁺ immune cells to PD-L1⁺ tumours by a bispecific antibody potentiates T-cell response and tumour control

  Xiaopei Cui, et al., bioRxiv - Immunology 2023

  Quote: ... Binding of anti-TIGIT antibodies HB0036/HB0030 to cell surface was detected by APC-anti his (Biolegend/362605). For ELISA based assay of anti-TIGIT antibodies in tumour digest ...
• Modified E2 glycoprotein of hepatitis C virus enhances pro-inflammatory cytokines and protective immune response in mice

  Vijayamahantesh, et al., bioRxiv - Microbiology 2022

  Quote: Isolated CD4+T cells from healthy donors were incubated with sE2 or sE2F442NYT in the presence of 5µg/ml human anti-CD3 antibody and 1µg/ml human anti-CD28 antibody (BioLegend) for 2 hrs ...
• Predicting efficacy of immunotherapy in mice with triple negative breast cancer using a cholesterol PET radiotracer

  Nicholas G. Ciavattone, et al., bioRxiv - Immunology 2023

  Quote: ... specific antibodies included anti-mouse CD3ε (145-2C11) and CD28 (37.51) or anti-human CD3 antibody (OKT3) and anti-human CD28 (CD28.2) (antibodies from Biolegend). For flow cytometry experiments ...
• Membrane positioning across antigen-induced synaptic contacts tunes CAR-T cell signaling and effector responses

  Fenglei Li, Kaushik Choudhuri, bioRxiv - Immunology 2023

  Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
• γδ T cells are effectors of immune checkpoint blockade in mismatch repair-deficient colon cancers with antigen presentation defects

  Natasja L. de Vries, et al., bioRxiv - Cancer Biology 2021

  Quote: ... incubated with human Fc receptor block (BioLegend), and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1 ...
• A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia

  Richard A. Voit, et al., bioRxiv - Cell Biology 2022

  Quote: ... and anti-human CD45-APC (Biolegend, 368512). Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies ...
• A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia

  Richard A. Voit, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-human CD34-Alexa 488 (Biolegend, 343518) and anti-human CD235a-APC (Ebioscience ...
• A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia

  Richard A. Voit, et al., bioRxiv - Cell Biology 2022

  Quote: ... anti-human CD3-Pacific Blue (Biolegend, 344823), anti-human CD19-PECy7 (Biolegend ...
• Neuroprotection in early stages of Alzheimer’s Disease is promoted by Transthyretin angiogenic properties

  Tiago Gião, et al., bioRxiv - Molecular Biology 2021

  Quote: ... using the LEGENDplex Human Angiogenesis Panel (BioLegend) bead-based immunoassay ...
• Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations

  Linda K. Krasniewski, et al., bioRxiv - Cell Biology 2022

  Quote: ... PE anti-mouse/human CD11b Antibody (Biolegend, Cat# 101208 ...
• Holistic Characterization of Tumor Monocyte-to-Macrophage Differentiation Integrates Distinct Immune Phenotypes in Kidney Cancer

  Adriana M. Mujal, et al., bioRxiv - Immunology 2021

  Quote: Cells were incubated with Human FcX (Biolegend) to prevent non-specific antibody binding ...
• Potent neutralizing anti-SARS-CoV-2 human antibodies cure infection with SARS-CoV-2 variants in hamster model

  Maya Imbrechts, et al., bioRxiv - Immunology 2021

  Quote: ... FITC anti-human CD3 antibody (Biolegend, 300306) and PE streptavidin (Biolegend ...
• Engineered niches support the development of human dendritic cells in humanized mice

  Giorgio Anselmi, et al., bioRxiv - Immunology 2019

  Quote: ... and mouse anti-human CD45 (HI30, Biolegend) overnight followed by 1hr incubation at room temperature with goat anti-mouse Cy3 (Jackson laboratory ...
• Discovery and Engineering of a Therapeutic Interfering Particle (TIP): a combination self-renewing antiviral

  Elizabeth J Tanner, et al., bioRxiv - Synthetic Biology 2019

  Quote: ... and mouse anti human CD4 APC (Biolegend),
• Non-oncology drugs are a source of previously unappreciated anti-cancer activity

  Steven M. Corsello, et al., bioRxiv - Cancer Biology 2019

  Quote: ... and APC anti-human CD4 (Biolegend 300514). 68% of sgCD45-transduced cells expressed CD45 and 28% of sgCD4-transduced cells expressed CD4.
• Variation of immune cell responses in humans reveals sex-specific coordinated signaling across cell types

  Gabriela K. Fragiadakis, et al., bioRxiv - Immunology 2019

  Quote: ... blocked with human TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
• CIS checkpoint deletion enhances the fitness of cord blood derived natural killer cells transduced with a chimeric antigen receptor

  May Daher, et al., bioRxiv - Immunology 2020

  Quote: ... APC/Cy7 anti-human CD3 (HIT3a, Biolegend), Brilliant Violet 605™ anti-human CD56 (NCAM ...
• CIS checkpoint deletion enhances the fitness of cord blood derived natural killer cells transduced with a chimeric antigen receptor

  May Daher, et al., bioRxiv - Immunology 2020

  Quote: ... APC/Cy7 anti-human CD3 (HIT3a, Biolegend), Brilliant Violet 605™ anti-human CD56 (NCAM ...
• Human immunocompetent Organ-on-Chip platforms allow safety profiling of tumor-targeted T-cell bispecific antibodies

  S. Jordan Kerns, et al., bioRxiv - Cancer Biology 2021

  Quote: ... anti-human-CD8-PE/Dazzle-594 (BioLegend), and anti-human CD69 APC (BioLegend).
• Human immunocompetent Organ-on-Chip platforms allow safety profiling of tumor-targeted T-cell bispecific antibodies

  S. Jordan Kerns, et al., bioRxiv - Cancer Biology 2021

  Quote: ... anti-human CD4 Brilliant Violet 786 (BioLegend), anti-human-CD8-PE/Dazzle-594 (BioLegend) ...
• SARS-CoV-2 variant B.1.1.7 caused HLA-A2⁺ CD8⁺ T cell epitope mutations for impaired cellular immune response

  Chanchan Xiao, et al., bioRxiv - Immunology 2021

  Quote: ... and FITC anti-human Granzyme B (BioLegend) staining.
• The proteasome regulator PSME4 drives immune evasion and abrogates anti-tumor immunity in NSCLC

  Aaron Javitt, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse/human CD44-FITC (IM7, Biolegend 103005), FC ...
• TIGIT-Fc Promotes Antitumour Immunity

  Wenyan Fu, Changhai Lei, Jian Zhao, Shi Hu, bioRxiv - Bioengineering 2020

  Quote: ... and antibodies to human CD3 (HIT3a, BioLegend), CD45 (2D1 ...
• A high throughput bispecific antibody discovery pipeline

  Aude I. Segaliny, et al., bioRxiv - Bioengineering 2021

  Quote: ... FITC-anti human CD25 antibody (#356106, Biolegend), APC anti-human CD279 antibody (#329908 ...
• A high throughput bispecific antibody discovery pipeline

  Aude I. Segaliny, et al., bioRxiv - Bioengineering 2021

  Quote: ... PE-anti human CD69 antibody (#310906, Biolegend), FITC-anti human CD25 antibody (#356106 ...
• A high throughput bispecific antibody discovery pipeline

  Aude I. Segaliny, et al., bioRxiv - Bioengineering 2021

  Quote: ... APC anti-human CD279 antibody (#329908, Biolegend), PE/Cyanine 7 anti-human CD4 antibody (#317414 ...
• Control of Neutrophil activation through Semaphorin 7A-Plexin C1 is essential for immune defense during pulmonary sepsis

  Tiago Granja, et al., bioRxiv - Cell Biology 2021

  Quote: ... anti-human LFA1 (CD11a) FITC (Biolegend 363404) and anti-human PSGL1 BV421 (BD # 563961 ...
• Integrative profiling of early host chromatin accessibility responses in human neutrophils with sensitive pathogen detection

  Nikhil Ram-Mohan, et al., bioRxiv - Immunology 2020

  Quote: ... mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107), and mouse anti-human V500-CD45 (BD # 560779 ...
• Aged Breast Extracellular Matrix Drives Mammary Epithelial Cells to an Invasive and Cancer-Like Phenotype

  Gokhan Bahcecioglu, et al., bioRxiv - Cancer Biology 2020

  Quote: ... 0.3 μL human hashtag (HTO) antibody (Biolegend) was added to each sample (a different hashtag antibody was used for each sample/replicate ...
• Human bone marrow organoids for disease modelling, discovery and validation of therapeutic targets in hematological malignancies

  Abdullah O. Khan, et al., bioRxiv - Cancer Biology 2022

  Quote: ... anti-human CD34 (BioLegend Cat# 343502, RRID:AB_1731898), polyclonal VE-Cadherin (Thermo Fisher Scientific Cat# 36-1900 ...
• Multivalent, Bispecific αB7-H3-αCD3 Chemically Self-Assembled Nanorings Direct Potent T-cell Responses Against Medulloblastoma

  Ellie A. Mews, et al., bioRxiv - Cancer Biology 2022

  Quote: ... anti-human CCR7 (Biolegend, clone G043H7, AF647), and anti-human CD45RO (Biolegend ...
• Distinct gene expression by expanded clones of quiescent memory CD4⁺ T cells harboring intact latent HIV-1 proviruses

  Georg H J Weymar, et al., bioRxiv - Immunology 2022

  Quote: ... PacificBlue anti-human CD3 (BioLegend, cat. 300431), Brilliant Violet 605 anti-human TCR Cβ1 (BD ...
• Omicron BA.2 specifically evades broad sarbecovirus neutralizing antibodies

  Yunlong Cao, et al., bioRxiv - Immunology 2022

  Quote: ... FITC anti-human CD20 antibody (BioLegend, 302304), Brilliant Violet 421™ anti-human CD27 antibody (BioLegend ...
• LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer

  Donatella Delle Cave, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and anti-human CD44-APC (BioLegend – 338805) to identify CSCs ...
• Evolution of inflammation and immunity in a dengue virus 1 human infection model

  Adam T. Waickman, et al., bioRxiv - Immunology 2022

  Quote: ... and anti-human IgA HRP (Biolegend, 411,002). Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL ...
• Evolution of inflammation and immunity in a dengue virus 1 human infection model

  Adam T. Waickman, et al., bioRxiv - Immunology 2022

  Quote: ... and anti-human IgA HRP (Biolegend, 411,002). Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL ...
• Generation of PRKN and PINK1-KO and double KO cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing

  Carol X.-Q. Chen, et al., bioRxiv - Neuroscience 2022

  Quote: ... then blocked with Human TruStain FcX (Biolegend). Cells were stained with FACS antibodes (Table 2 ...
• A potent human monoclonal antibody with pan-neutralizing activities directly dislocates S trimer of SARS-CoV-2 through binding both up and down forms of RBD

  Xiaofei Wang, et al., bioRxiv - Immunology 2022

  Quote: ... APC-conjugated anti-human IgG (Fc) (Biolegend), APC-Cy7-conjugated anti-CD3 antibody (Biolegend) ...
• Aging leads to DNA methylation alterations associated with loss of lineage fidelity and breast cancer in mammary luminal epithelial cells

  Parijat Senapati, et al., bioRxiv - Genomics 2022

  Quote: ... and anti-human CD271 (Biolegend, clone ME20.4) by following standard flow cytometry protocol ...
• MAP1LC3C regulates lysosomal exocytosis and induces zinc reprogramming in renal cancer cells

  Rita Verma, et al., bioRxiv - Cell Biology 2022

  Quote: ... pacific blue anti-human CD31 (303113, Biolegend) and anti-human CD45 (368539 ...
• Identification of HIV-Reservoir Cells with Reduced Susceptibility to Antibody-Dependent Immune Response

  Antonio Astorga-Gamaza, et al., bioRxiv - Immunology 2022

  Quote: ... and anti-human HLA-DR (BV711, Biolegend) antibodies were used ...
• Multivalent, Bispecific αB7-H3-αCD3 Chemically Self-Assembled Nanorings Direct Potent T-cell Responses Against Medulloblastoma

  Ellie A. Mews, et al., bioRxiv - Cancer Biology 2022

  Quote: ... anti-human CD8 (Biolegend, clone SK1, BV711), anti-human CD25 (Biolegend ...
• Human primed endothelial colony forming cells exert neuroprotective effects in the growth restricted newborn piglet

  Kirat K. Chand, et al., bioRxiv - Neuroscience 2021

  Quote: ... human CD45-FITC (BioLegend; dilution 1:25) and human CD31-V450 BD Biosciences ...
• Global Absence and Targeting of Protective Immune States in Severe COVID-19

  Alexis J. Combes, et al., bioRxiv - Immunology 2020

  Quote: ... and Human TruStain FcX block (BioLegend; #422302) were used to block pre-bound antibodies and Fc receptors ...
• CAR T cell entry into tumor islets is a two-step process dependent on IFNγ and ICAM-1

  Chahrazade Kantari-Mimoun, et al., bioRxiv - Immunology 2020

  Quote: ... AF647-anti-human CD3 (clone SK7, BioLegend), PE/Dazzle or BV421-anti-human EGFR (clone AY13 ...
• Structurally and functionally distinct early antibody responses predict COVID-19 disease trajectory and mRNA vaccine response

  Saborni Chakraborty, et al., bioRxiv - Immunology 2021

  Quote: ... and blocked with Human TruStain FcX (BioLegend) to reduce nonspecific binding ...
• Long-lasting germinal center responses to a priming immunization with continuous proliferation and somatic mutation

  Jeong Hyun Lee, et al., bioRxiv - Immunology 2021

  Quote: ... mouse anti-human CD20 BV785 (2H7, Biolegend), mouse anti-human PD1 BV605 (EH12.2H7 ...