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Citations for BioLegend :
251 - 300 of 6666 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... B cells were also stained with following anti-human antibodies: anti-IgD-BV421 (Biolegend, 348226), anti-CD27-FITC (BD biosciences ...
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bioRxiv - Systems Biology 2020Quote: ... CD4+ were purified by negative selection using magnetic human CD4+ T cells nanobeads (MojoSort, Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed in PBS and incubated for 1hour with secondary anti-human FITC (Biolegend) at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Up to 60,000 sorted live synovial cells were pre-incubated with Human TruStain FcX (BioLegend) in Cell Staining Buffer (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with PE labeled anti-human IgG Fc secondary antibody (Biolegend) at a 1:20 dilution in 50 μl staining buffer at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended at 106 cells/mL and then treated with Human TruStain FcX blocker (BioLegend 422302). Cell sorting was carried out in azide-free buffers ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated with 40 ng/ml human IL-10 (BioLegend, San Diego, CA, USA) for 2 h at 4°C and cells were collected for flow cytometric analysis.
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bioRxiv - Cancer Biology 2019Quote: ... the cells were fixed and stained by anti-human mAb FOXP3-PE antibody (BioLegend, CA) and anti-human mAb CD4–FITC clone RPA-T4 (BioLegend ...
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bioRxiv - Genetics 2020Quote: ... Bone marrow cells were first incubated for 15 minutes with Human TruStain FcX (BioLegend #422302) and TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Genetics 2021Quote: ... The cell suspension was incubated in Human TruStain FcX blocking solution (1:20, Biolegend; 422302) at room temperature for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... confluent BCR-expressing B cells were FACS sorted on IgM (APC anti-human IgM; BioLegend) and kappa light chain (PE anti-human kappa light chain ...
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bioRxiv - Immunology 2021Quote: ... and 1×106 cells were incubated sequentially or together with Human Trustain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406) ...
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bioRxiv - Immunology 2020Quote: ... with 100ug of antibodies against specific human NK cells expressing NKp46 Domain 1 (Biolegend, 9E2) and/or human CD8α (Bioxcell ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with PBS and then incubated with Human TruStain FcX Blocking Solution (Biolegend) for 10 mins ...
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bioRxiv - Genomics 2022Quote: ... Ligand expression was confirmed for engineered 293T cells using anti-human CD28-PE (302907, Biolegend), anti-human CD20-PE (302306 ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with anti-human IgG-APC (clone M1310G05, 1/250 dilution, BioLegend) in PBS-BSA for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were then resuspended in 50µL ice-cold 1:10 human TruStain FcX (BioLegend 422302) in MACS buffer and incubated for at least 5 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... The TALL-104 cells were first stimulated with anti-human CD3 antibody (5μg/ml) (Biolegend) and anti-human CD28 antibody (5μg/ml ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with anti-CD1a (FITC anti-human CD1a Antibody, clone: HI149, BioLegend), anti-CD93 (PE conjugated anti-human CD93 Antibody ...
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bioRxiv - Immunology 2023Quote: ... 100 μL of cell suspension was mixed with Pacific Blue anti-human Ki-67 (Biolegend), followed by incubation at room temperature in the dark for 30 min ...
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bioRxiv - Immunology 2023Quote: ... for human samples and subsequently stained with live/dead cell exclusion dye (Zombie Dyes, BioLegend). Surface staining was performed with fluorophore-conjugated antibodies (Table S1 ...
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used to stain the cells: BV711-conjugated anti-human CD4 (BioLegend); APC-conjugated anti-human PD1 (BioLegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed and permeabilized cells were stained with PE anti-human Ki-67 antibody (BioLegend #350504) diluted 1:10 in the BD Perm/Wash buffer from the kit for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated overnight at 4 °C with the primary antibodies against ASC (HASC-71, BioLegend). Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed and incubated in primary conjugated antibodies (EZH2, BD Bioscience; CD133, Miltenyi; CD15, Biolegend; Sox2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFN◻ by ELISA (BioLegend). All assays were performed in triplicate and average IFN◻ production was plotted with standard deviation.
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bioRxiv - Immunology 2021Quote: ... Human Fc receptors (FcRs) were blocked using Human TruStain FcX™ (BioLegend). In samples stained with multiple BD Horizon Brilliant reagents ...
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bioRxiv - Cancer Biology 2023Quote: Human PBMCs were treated with Fc blocking solution (Human TruStain FcX, Biolegend) and stained with the following fluorescent antibodies from Biolegend (unless otherwise stated) ...
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... CD81 expression was measured 7 days post-transduction by dislodging cells with TrypLE and live cells were stained with APC-conjugated anti-human CD81 antibody (Biolegend 349509). CD81 expression was assessed on a BD LSRII with >90% of Calu3-CRISPRi cells with CD81 knocked down compared to a non-targeting sgRNA control.
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend, Cat no.480045). Purity was consistently over >75% CD8+ with no contamination of CD4+ T cells and >80% of CD8+ T cells being naive ...
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bioRxiv - Immunology 2023Quote: Cytokine levels in the supernatants from B cells that had been stimulated for 48 hr were measured using the LEGENDplex™ Human B cell Panel (13-plex) (Biolegend), using the same procedure as described for the antibody measurements ...
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bioRxiv - Cell Biology 2023Quote: ... to assess viable cells and fluorophore-conjugated monoclonal antibodies to the following human cell-surface markers: anti-CD45-BV650 (Biolegend, 304044), anti-CD206-AF647 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
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bioRxiv - Immunology 2021Quote: ... human CD4-APC (Biolegend), human FoxP3-APC (eBioscience) ...
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bioRxiv - Neuroscience 2021Quote: ... Human TruStain FcXTM (Biolegend). Protected from light ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD45 (BioLegend #304014), human CD25 (BioLegend #302606) ...
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bioRxiv - Cancer Biology 2023Quote: ... human FOXP3 (BioLegend #320116), Alexa Fluor® 647-conjugated anti-mouse integrin αvβ5 Ab (clone ALULA ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD45 (BioLegend #304014), Zombie NIR™ Fixable Viability Kit (BioLegend #423106 ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD25 (BioLegend #302606), human FOXP3 (BioLegend #320116) ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD8 (BioLegend #344712), human CD45 (BioLegend #304014) ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD3 (BioLegend, #317324), mouse CD45 (BioLegend #103130) ...
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bioRxiv - Immunology 2024Quote: ... Human TruStain FcX (BioLegend) was added at 1:20 to block Fc receptors for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... human TGFβ1 (580706; BioLegend), human IL-12 (219-IL;R&D Sys-tems) ...