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Citations for BioLegend :
251 - 300 of 2782 citations for L lactate dehydrogenase B chain LDH B Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... This reference curve was established in each experiment for all FcyR-specific antibodies of interest (PE-conjugated clone 10.1 to detect FcyRI, clone AT10 to detect FcyRIIa/b and clone 3G8 to detect FcyRIIIa, all from Biolegend) and used to calculate receptor numbers based on fluorescence intensity of FcyR staining on CHO cells ...
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... about 1-2e6 mononuclear cell suspension was stained with a panel of Total-seq B antibodies (1ug/each, Biolegend) and fluorochrome conjugated flow antibodies for CD45 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β (432604) and Cathepsin B (EA100429) enzyme-linked immunosorbent assay (ELISA) kits were from BioLegend (San Diego, CA) and Origene (Rockville ...
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bioRxiv - Cancer Biology 2021Quote: ... NKG2D ligands were blocked on the cancer cell lines and single cells of tumor organoids by incubating the target cells with 12 µg/mL anti-MICA/B (clone 6D4, BioLegend), 1 µg/mL anti-ULBP1 (clone 170818 ...
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bioRxiv - Genomics 2020Quote: ... loss of MHC class I expression in B2M knock-outs was assessed by staining with FITC-conjugated anti-HLA-A,B,C (W6/32, Biolegend). To maximize surface expression of IFNγ regulated proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... MNCs were harvested by ficoll gradient centrifugation and human hematopoietic cells were identified by flow cytometry using the following antibody cocktail: HLA-A/B/C FITC (clone W6/32, Biolegend), mouse CD45.1 PE-CY7 (clone A20 ...
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Systems Biology 2022Quote: ... The population corresponding to cluster B was sorted by top 1% of CD49c abundance via red florescent antibody (Biolegend 343808). Cells corresponding to cluster A were sorted from 25-75% abundance range ...
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bioRxiv - Immunology 2021Quote: Viability of stimulated B cells were assessed by flow cytometry using FITC-labeled annexin V and propidium iodide (PI) (Biolegend). For apoptosis assays by live-imaging ...
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bioRxiv - Immunology 2020Quote: ... ABCs were isolated from purified total B cells by CD11c positive selection using a biotin conjugated CD11c antibody (301612, Biolegend) and anti-biotin magnetic beads ...
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bioRxiv - Immunology 2021Quote: ... for 15 min at RT and stained intracellularly with Fix/Perm Buffer B and antibodies for TNF (PE-Cy7, clone MAb11, BioLegend), IFN-γ (BV711 ...
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bioRxiv - Immunology 2021Quote: ... PD-1 and CD25 expression and cell viability and cell expansion were quantified by live/dead staining and the following antibodies via flow cytometry: anti-TCRa/b-PE (306708, BioLegend), anti-CD3-AF700 (11-0039-42 ...
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bioRxiv - Immunology 2021Quote: ... followed by selection of memory B cells by positive expression of CD20 and CD27 (CD20-FITC, clone 2H7; CD27-APC-Cy7, clone O323, both BioLegend) but not immunoglobulin D (IgD-PE ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured for 4 h at 37°C in B cell medium containing 1x Cell Activation Cocktail (without Brefeldin A) (BioLegend) and 3h with 1x Protein Transporter Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then processed for single cell RNAseq as described in (Peng et al., 2019) using Total Seq B antibodies and the recommended protocol from Biolegend. Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Immunology 2022Quote: ... Cat# 137024), granzyme B (QA18A28, Cat# 396413), TNF (MP6-XT22, Cat# 506329), IFN-γ (XMG1.2, Cat# 505846) were obtained from BioLegend. Anti-Foxp3 (FJK-16s ...
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bioRxiv - Immunology 2022Quote: The following antibodies were used to detect B cell populations in the BM: brilliant violet (BV) 421 anti-mouse CD19 antibody (Clone 6D5, BioLegend), PE/Cyanine5 anti-mouse/human CD45R/B220 (Clone RA3-6B2 ...
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bioRxiv - Immunology 2022Quote: ... The PBMCs were fixed and permeabilized using Foxp3 / Transcription factor kit (eBioscience) and stained intracellularly with anti-Granzyme-B-Alexa-Fluor-700 (Biolegend), anti-TNF-α-efluor-450 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... CXCR5-PE-Dazzle 594 (J252D4, 1:40), and CD38-PE-Cy7 (HIT2, 1:200) for GC B cells (all BioLegend). For donors 321-07 and 321-08 the PBMC samples for days 0 and 5 were stained with TotalSeq-C anti-human hashtag oligos 9 and 10 ...
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bioRxiv - Immunology 2023Quote: Activated and memory B cells were enriched from PBMCs by first staining with IgD-PE and MojoSort anti-PE Nanobeads (BioLegend), and then processing with the EasySep Human B Cell Isolation Kit using the EasyEights magnet (Stemcell ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Immunology 2023Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin, BioLegend, 405273), 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... Every 106 B cells in 100 μl solution were stained with 3 μl FITC antihuman CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μl Brilliant Violet 421 antihuman CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... YU2-gp140 fold-on, and hepatitis B surface antigen (HBs Ag) (Protein Specialists, hbs-875) conjugated to streptavidin Alexa Fluor 647 (Biolegend, 405237), streptavidin PE (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... and 7.5 nM of either a) tail-biotinylated cognate pMHC complex or b) biotinylated Armenian hamster anti-CD3ε antibody (clone 145-2C11, Biolegend 100304). ICAM-1 and pMHC proteins were expressed and purified as described (Wang et al. ...
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bioRxiv - Microbiology 2020Quote: ... anti-His PE (Biolegend), and human Hashtag 3 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... LDH-Cytox™ Assay Kit was purchased from Biolegend (Cat. No. 426401). The LEGENDplex™ Mouse Immunoglobulin Isotyping Panel (6-plex ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse Ig light chain lambda APC # 407306 and anti-mouse IgG light chain kappa PE both from Biolegend, San Diego ...
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Molecular Biology 2022Quote: ... samples P008-016 and P048-119 (Suppl. Fig. 4D) were labelled with TotalSeq B Hashtag antibodies 1-4 (Biolegend, clone LNH-94) during the staining procedure described above and pooled in groups of four.
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Immunology 2021Quote: ... a set of plated moDCS were treated with an anti-HLA-A/B/C antibody (40 μg mL−1, Clone W6/32, Biolegend Cat 311428) or anti-class II (10 μg mL−1 ...
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bioRxiv - Microbiology 2022Quote: ... then stained for 30 minutes at 2-8°C with the following cytokine antibodies: Granzyme B (GzB) BV421 (4 μg/mL, BioLegend Cat. 396414), IFN-gamma (IFN-γ ...
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bioRxiv - Immunology 2023Quote: Serum BAFF quantitation was performed on plasma samples from 12-14 week-old Sh2b3E372K mice using the LEGENDplex Mouse B cell Panel – S/P (1-plex) w/VbP kit (BioLegend; cat # 740983) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Immunology 2019Quote: ... PE anti-mouse TCR β chain (Biolegend, 109208), APC anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... anti–mouse Ig light chain κ (Biolegend, 409506), 5% normal mouse serum (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... anti-non-muscle myosin heavy chain II (BioLegend, clone Poly19098 ...
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bioRxiv - Immunology 2023Quote: ... and anti-His PE (Biolegend), at 4 °C for 1 h ...
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bioRxiv - Immunology 2020Quote: Single live cells from individual mice were isolated by flow cytometry and labeled with TotalSeq-B cell hashing antibodies (Biolegend, San Diego, CA). Labeled cells were subjected to 3’ version 3.1 transcriptome library preparation using the Chromium 10X system (10X Genomics ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Bioengineering 2021Quote: ... The His tagged ACE2 variants were detected using anti-His mAb (Cat. No. 652505, BioLegend).
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bioRxiv - Immunology 2022Quote: ... or 50ng/ml anti-human ICOS-L or 50ng/ml of the corresponding isotype control (Biolegend). After 60 min ...