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Citations for BioLegend :
251 - 300 of 4751 citations for IL 12 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Microbiology 2023Quote: ... IL-1β and IL-6 were analyzed by ELISA MAX Deluxe Sets (Biolegend) according to manufacturer’s instruction.
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Immunology 2021Quote: ... Induced GC B-cells were then replated onto a fresh CD40LB feeder line and cultured with mouse IL-21 (10 ng/mL; BioLegend) or mouse IL-4 for another 3 to 4 days.
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... and IL-23 (433704, BioLegend) levels were assessed by ELISA ...
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bioRxiv - Immunology 2022Quote: ... and IL-6 (BioLegend, 570804). To differentiate M2 macrophages ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (MQ1-17H12, Biolegend), and IFNγ (4S.B3 ...
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bioRxiv - Immunology 2019Quote: ... IL-4-PERCP Cy5.5 (Biolegend) and IFNγ-eFlour450 (ebioscience ...
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bioRxiv - Immunology 2019Quote: ... anti-IL-23 (MMp19B2, BioLegend) and anti-IL-1R (JAMA-147 ...
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bioRxiv - Immunology 2021Quote: ... IL-17A (TC11-18H10.1; BioLegend), and FOXP3 (MF-14 ...
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bioRxiv - Immunology 2021Quote: ... IL-8 (BioLegend cat. 78141), TGF-β (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Recombinant murine IL-4 (Biolegend) was combined with rat IgG1 mAb to IL-4 ...
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bioRxiv - Immunology 2021Quote: ... IL-10 (JESS-16E3; BioLegend), IL-17A (TC11-18H10.1 ...
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bioRxiv - Microbiology 2021Quote: ... IL-4 APC (Biolegend, #504106), IL-17 PECy7 (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... IL-17 PECy7 (Biolegend, # 506922), donkey anti-mouse IgG conjugated to AlexaFluor 647 (Invitrogen #A-31571 ...
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bioRxiv - Immunology 2022Quote: ... CD4 and IL-17 (Biolegend).
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bioRxiv - Immunology 2022Quote: ... and IL-10 (#431411, BioLegend) were determined using ELISA after 48 hr of stimulation ...
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bioRxiv - Neuroscience 2021Quote: ... IL-1β ELISA kit (Biolegend) was used for determination in plasma and ELISA was performed according to manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-22 (POLY5164, Biolegend, #516411); RORγT (B2D ...
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bioRxiv - Immunology 2020Quote: ... and IL-22 (Biolegend, #436304), and of human IL-17A (Invitrogen #BMS2017 ...
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bioRxiv - Immunology 2020Quote: Recombinant murine IL-33 (Biolegend) was injected intraperitoneally to C57BL/6 mice (100 ng/mouse) ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... IL-1β and IFNγ (BioLegend) secretion was measured in by ELISA following the manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... IL-12p23 (clone C15.6, Biolegend), pro-IL-1β (clone NJTEN-3 ...
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bioRxiv - Immunology 2020Quote: ... injection of IL-33 (Biolegend) at a dose of 12.5ug/kg body weight ...
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bioRxiv - Immunology 2020Quote: ... IL-2 PE (Biolegend 503808), CD4 PE-Cy7 (Biolegend 100528) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 BV605 (Biolegend 500332), and Live/dead Near-IR (Invitrogen L10119) ...
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bioRxiv - Immunology 2021Quote: ... IL-6 (Biolegend, cat # 501101) and CD16/32 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... AF488-IL-4 (11B11, Biolegend), PerCP-eFluor710-IL-13 (eBio13A ...
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bioRxiv - Immunology 2022Quote: ... and IL-17A-PE (Biolegend), and dead cells were excluded using Zombie NIR (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... and IL-1β (Biolegend, 432601) were measured by ELISA according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... IL-4 (clone 11B11, BioLegend), IL-17a (clone eBiol7B7 ...
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bioRxiv - Immunology 2022Quote: ... PE-IL-5 (TRFK5, Biolegend), PE-CF594-KLRG1 (2F1 ...
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bioRxiv - Immunology 2023Quote: ... and recombinant IL-17A (BioLegend). Streptavidin HRP (BD Pharmingen ...
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bioRxiv - Neuroscience 2023Quote: ... IL-6 ELISA (Biolegend, 431304); Histamine ELISA (Beckman Coulter ...