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Citations for BioLegend :
251 - 300 of 6270 citations for Gem Associated Protein 6 GEMIN6 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against IL-1α (503207, Biolegend), IL-1β (12426 ...
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bioRxiv - Immunology 2022Quote: ... All enriched samples were stained with the following panel of TotalSeq-A-labeled anti-mouse antibodies to detect protein expression in the scRNA-seq dataset: Ly6C (HK1.4, BioLegend), CD44 (IM7 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% BSA in Tris-buffered saline/0.05% Tween-20 and proteins were detected by specific primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), MOBP (bs-11184R ...
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bioRxiv - Microbiology 2020Quote: ... The levels of murine IL-6 and TNF-α and human IL-6 were measured using ELISA MAX™ kits (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: Antibody levels in the supernatants from B cells that had been stimulated for 6 days were measured using the LEGENDplex™ Human Immunoglobulin Isotyping Panel (6-plex) (Biolegend), a bead-base immunoassay ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 times and then incubated with Avidin-HRP (Biolegend, 405103) for biotinylated probes at 1:2000 dilution for 45 minutes at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Polyclonal HRP conjugated goat anti-mouse IgG (BioLegend, London, UK) was diluted 1 in 1000 and 100 µl was added to each well ...
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bioRxiv - Biochemistry 2021Quote: ... wells were probed with HRP-streptavidin (Biolegend 405210, 1000:1) or biotinylated anti-a αvβ3 antibody (Biolegend 304412 ...
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bioRxiv - Immunology 2022Quote: ... Primary Abs were followed by HRP-conjugated secondary Abs (BioLegend), and membranes were developed with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and anti-RpoB-HRP (loading control, dilution = 1:5,000; Biolegend) for 1 hour at room temperature with mild agitation ...
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bioRxiv - Bioengineering 2023Quote: ... Blots were then incubated with HMGB-1-HRP (BioLegend 651411) overnight ...
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bioRxiv - Bioengineering 2024Quote: ... then washed in PBS and incubated with streptavidin-HRP (BioLegend) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 50 µl of anti-His HRP (1:3000) (Biolegend 652404) in dilution buffer was added to each well and incubated at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... activated T-cells were treated with HDACI as described before and after 72 h total protein acetylation of the cells was determined by flow cytometry using PE-coupled anti-Acetylated Lysine Antibody (Biolegend). Cells were washed with PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
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bioRxiv - Immunology 2022Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Genomics 2022Quote: ... The custom oligos were synthesized by Integrated DNA Technologies and conjugated to anti-Nuclear Pore Complex Proteins antibody (Clone Mab414, BioLegend) by BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins from the native gel were transferred to the PVDF membrane and probed with an anti-His-tag antibody (BioLegend). For BN-PAGE ...
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bioRxiv - Immunology 2022Quote: ... and Mouse IL-6 ELISA MAXTM Deluxe Set (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... or 6 μg/mL anti-ICOS (clone C398.4A, Biolegend) in PBS overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... clone HI9a (6 µg/mL, BioLegend, San Diego, USA); anti-CD63–APC ...
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bioRxiv - Immunology 2020Quote: ... anti-MHC class I (clone 28-8-6, BioLegend), anti-β-catenin (clone D2U8Y ...
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bioRxiv - Cell Biology 2021Quote: ... and CD309/Kdr/Flk-1 (7D4-6)-APC (BioLegend). 4’ ...
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bioRxiv - Immunology 2024Quote: ... and cytokine stains with IL-6 (MP5-20F3; Biolegend), IL-10 (JES5-16E3 ...
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bioRxiv - Biochemistry 2023Quote: IL-6-APC (BioLegend, 504508, MP5-20F3, 1 μL)
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bioRxiv - Immunology 2022Quote: ... or α-IL-6 (BioLegend, 1:100, MP5-20F3) antibodies for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 ng/mL recombinant human IL-6 (BioLegend #570804), 50 ng/mL recombinant human IL-8 (BioLegend #574204) ...
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bioRxiv - Immunology 2020Quote: ... Detection reagents were anti-mouse IgG-HRP (all BioLegend, London, UK). Optical densities were measured at 450 nm using a SpectraMAX i3 microplate reader (Molecular Devices ...
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bioRxiv - Bioengineering 2021Quote: ... Avidin-HRP (100 μl, cat. #405103, Biolegend, San Diego, CA, USA) was diluted 500 times in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated streptavidin (BioLegend) for PLA2G12A or HRP-conjugated sheep anti-mouse IgG F(ab’)2 fragment (Amersham ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting homogenate was immunodepleted to remove soluble amyloid beta or mock immunodepleted using protein A agarose beads (Thermo, 20334) with 4G8 antibody (Biolegend, 800703) or mouse serum (Merck ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Immunology 2023Quote: ... HTO stained PBMCs from all 10 donors were then pooled and stained with oligo-tagged TotalSeq C antibody for cell surface proteins for T cell memory markers (CD45RA, CCR7, CD95 and IL7Rα) (0.5 µg/condition; Biolegend, Table S2B) for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD309 (VEGFR2) (Biolegend, clone 7D4-6; 1:50); anti-human CD56 (NCAM ...
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bioRxiv - Neuroscience 2021Quote: ... anti-Pax 6 (1:100 901301; BioLegend, San Diego, CA), anti-Tuj1 (801202 ...
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bioRxiv - Neuroscience 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) (diluted 1:20’000; BioLegend, #422801). Finally ...
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bioRxiv - Microbiology 2021Quote: ... and intracellularly stained with anti-IL-6 (PE-Cy7, BioLegend), and anti-CXCL10 (PE ...
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bioRxiv - Immunology 2022Quote: ... Permeabilized cells were intracellularly stained with Bcl-6 (Biolegend 358512). Cells were acquired on Attune NxT Flow Cytometer and analyzed with Attune NxT software v 4.2.0.
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bioRxiv - Cancer Biology 2022Quote: ... anti-H-2Kb/H- 2Db (clone 28-8-6, Biolegend), anti-CD86 (clone GL-1 ...
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bioRxiv - Immunology 2021Quote: ... 40ng/ml rmGM-CSF and 40ng/ml rmIL-6 (BioLegend), with or without 50% E.G7-OVA supernatant ...