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Citations for BioLegend :
251 - 300 of 1262 citations for Ethyl 7 oxo 7 2 thiomorpholinomethyl phenyl heptanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... purified ILC2 isolated from IL-33-challenged mice were cultured overnight with 10μM TOFA in complete media supplemented with 20 ng/ml IL-7 (Biolegend). Cells were washed with assay medium supplemented with 10 mM glucose ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Cancer Biology 2023Quote: ... MEFs were trypsinized and stained with Apotracker-Green (Apo-15 peptide) and 7-AAD according to manufacturer’s instructions (Biolegend) to detect apoptotic cells ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... Cells were then filtered through 35 μm nylon mesh and stained with Annexin V-FITC and 7-AAD (Biolegend). 50,000 cells per sample were collected for analysis using a LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... apoptosis of the Neuro-2a cell was analyzed by Annexin-V-FITC and 7-AAD staining accordingly to the manufacturer’s instruction (BioLegend). Cells were immediately acquired on a Canto II flow cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were labeled with antibodies detecting the following cell-surface markers: CD133-APC (Biolegend, clone 7, dilution 1:20), CD19-PECy7 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... then resuspended in 200 µL of binding buffer and stained with 5 µL of 7-AAD (Cat#: 420403, Biolegend) for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Intracellular stains were performed using commercially available Fix and permeabilization solutions coupled to incubation with Ki-67 and 0.5 µg /mL 7-AAD (Biolegend) or Pmp70/Abcd3.
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bioRxiv - Immunology 2024Quote: ... Glutamax and HEPES and Thy1.1+ cells (stained with anti-Thy-1.1 PE-Cy7, clone: OX-7, BioLegend Cat# 202518) were sorted twice over 10 passages on a CytoFLEX SRT Cell Sorter (Beckman).
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bioRxiv - Immunology 2021Quote: RAW264.7 cells of BMDMs were collected after washing in ice-cold PBS and then detaching the cells with Accutase (BioLegend). Prior to collection ...
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bioRxiv - Immunology 2021Quote: ... processing omitted the 7-AAD step and incubated with a fixable live dead dye prior to incubation with fixation buffer (Biolegend) (15 mins) ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... or biotin, 2G5, Beckman Coulter, or Ber-ACT8, Brilliant Violet 711, BUV395, BD Biosciences, or Ber-ACT8, PE-Cy-7, Biolegend), KIR2DL1 (FAB1844F ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD71 expression in TF-1 and DAMI cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD71 (BioLegend) (#113811) ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD11b expression MDS92 and MDS-L cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD11b (BioLegend) (#557743) ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and either activated them with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and stimulated T cells with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used (mouseCD45-FITC from BD bioscience and 7-AAD, humanCD45-PE/Cy7, humanCD19-PE, humanCD3-APC and humanCD14-APC/Cy7 from Biolegend) and cells analyzed on the LSRFortessa flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then labeled according to the manual of the FITC ANNEXIN V Apoptosis Detection Kit with 7-AAD (BioLegend) with a modification where the amount of 7-AAD was doubled.
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bioRxiv - Microbiology 2021Quote: ... Cells were then filtered using a 40 μm cell strainer and stained with the Live/Dead dye 7 Amino-actinomycin D (7AAD, Biolegend) and antibodies against CD45.2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected and stained with fluorescently labeled antibodies against CD45 PE-Cy 7 (clone: 30-F11, cat # 103114: Biolegend), TCRβ APC (clone ...
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bioRxiv - Immunology 2023Quote: ... Mouse monoclonal anti-V5 (BLD-680605, clone 7/4) and rabbit polyclonal anti-V5 (BLD-903801) were obtained from BioLegend. Mouse monoclonal anti-ICP36 (anti-UL44 ...
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bioRxiv - Immunology 2024Quote: ... clone GK1.5-7; WEHI Antibody Facility); anti-IgD (1:200; clone 11-26c; eBioscience) and anti-GL7 (1:100; clone GL7; Biolegend).
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 500 xg for 7 minutes and resuspended in 1 mL ice cold Cell Stain Buffer (CSB, BioLegend). Samples were filtered through a sterile pre-wet 70 μm mesh ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained for 30 mins at 4°C in the dark with anti-human antibodies specific for anti-CD14 [APC/Cyanine-7] (301819, BioLegend), anti-CD16 [Fluorescein isothiocyanate (FITC)] (360715 ...
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bioRxiv - Molecular Biology 2020Quote: ... together with the trypsinized cells were harvested into tubes and samples were prepared on ice for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (1 × 106) were stimulated for 7 days at 37°C with anti-mouse CD3 (BioLegend; 1 μg/mL) and CD28 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: Apoptotic and necrotic cell analysis was performed using Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, #640926) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: The flow cytometry staining for necrosis and apoptosis was was performed with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640922). Once the cells were incubated in 10 µM of EdU ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2022Quote: Ten 10-week-old male wild type C57BL/6J mice were intravenously injected with an APC/Cyanine 7-conjugated anti-mouse CD45.2 antibody (Clone 104, BioLegend 109824, RRID: AB_830789) five minutes prior to euthanasia by CO2 inhalation ...
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bioRxiv - Cancer Biology 2021Quote: ... and early/late apoptosis was measured by using the PE Annexin V apoptosis detection kit with 7-AAD (Biolegend, San Diego, USA) according to the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
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bioRxiv - Immunology 2024Quote: ... in a 5% CO2 incubator at 37 °C for 7 days with 30 ng/ml IL-15 (BioLegend, San Diego, CA, USA) and 10 ng/ml IL-12 (Peprotech ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell RNAseq data for the 7 PDA cell lines were generated as following: Cell lines were trypsinized and resuspended in Cell Staining Buffer (Biolegend, Cat. No. 420201) and incubated with proper Total Seq B antibodies using recommended protocol from Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
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bioRxiv - Immunology 2024Quote: ... The cells were expanded for additional 3 days and Siglec+ population was sorted by staining cells with anti-Siglec-7 APC (BioLegend, clone 6-434) or anti-Siglec-9 APC (BioLegend ...
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bioRxiv - Molecular Biology 2023Quote: ... Murine anti-V5-tag (clone 7/4, #680602) and rabbit anti-V5-tag (Polyclonal, #903801) antibodies were purchased from BioLegend (San Diego, CA, USA). Anti-V5-tag mAb-magnetic beads (#M167-11 ...
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bioRxiv - Immunology 2024Quote: ... and live/dead staining was carried out with the 7-AAD-Viability Staining Solution (5 µg/ml) according to manufacturer instructions (BioLegend, San Diego, California, United States). Cell Sorting was performed using the CytoFlex SRT Benchtop Cell Sorter (Beckmann Coulter Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... or phosphorylated ERK1/2 (pERK1/2; Biolegend), cells were stained with surface markers then fixed with Paraformaldehyde 1% ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (Biolegend), CD127 ...
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bioRxiv - Immunology 2024Quote: ... Bcl-2 (BioLegend) or Ki67 (BD Bioscience ...