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Citations for BioLegend :
251 - 300 of 331 citations for ALLYL 11 12 EPOXY STEARATE TECH since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... mouse anti-human CD47 antibody conjugated to APC (323123) and anti-HA Alexa Fluor® 488 anti-HA.11 Epitope Tag (901509) were purchased from Biolegend. The antibody to ORF3a was a gift from Prof ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blotting was performed using an anti-HA mouse monoclonal antibody (Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody, BioLegend) as primary antibody diluted 1:1000 in TBS-T (20 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: At passages 11-13 pericytes at 90-95% confluence from T-75 cell culture flasks were dissociated with Accutase® Cell Solution (BioLegend) and prepared for flow cytometry to analyze subtype distribution in vitro ...
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bioRxiv - Immunology 2022Quote: ... anti-mIFNγ and anti-mIL-12 at 10 μg/mL (from Biolegend except rhIL- 2 ...
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bioRxiv - Pathology 2021Quote: ... pan aSyn (N-terminal directed, SYN303 [aa 2-12 Cat# 82401, Biolegend] ...
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bioRxiv - Immunology 2024Quote: ... anti-CD62L (eBioscience, 12-0621-81; Cytek, 60-0621-U025; Biolegend, 104436), anti-CD44 (Biolegend ...
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bioRxiv - Biophysics 2020Quote: The antibodies used for these experiments were mouse purified anti-HA.11 Epitope Tag monoclonal (clone 16B12) (Biolegend 901502; 1:500 dilution), rabbit affinity-isolated anti-FLAG polyclonal (Sigma-Aldrich F7425 ...
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bioRxiv - Immunology 2021Quote: ... or restimulated on day 11 in 96-well plates with 2.5ug/ml recombinant SARS-CoV-S protein S1 (Biolegend 792906, carrier-free) for 8h in the presence of 1ug/ml Golgiplug and 0.7ug/ml Golgistop ...
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed twice with PBSF and labeled with 50 μL of APC-conjugated monoclonal mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, Cat # 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Physiology 2022Quote: ... with utilization of PE rat IgG2a K isotype Control (BioLegend#12-4321-80) to determine the percentage of ATM cells ...
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bioRxiv - Immunology 2024Quote: ... H2-Dd-FITC (1:200; clone 34-2-12; BioLegend Cat. No. 110606), H2-Kd-APC (1:160 ...
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bioRxiv - Immunology 2024Quote: ... and 10 μg/mL anti-mouse IL-12/23p40 antibody (clone C17.8, BioLegend)).
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... Analytes were measured using the 12-plex LegendPlex Human Th Cytokine Panel(BioLegend) or the 13-plex LegendPlex Human Proinflammatory Chemokine Panel 1(BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Cytokines were quantified using the LEGENDplex 12-plex Human Th Cytokine Panel (BioLegend) according to the manufacturer’s protocol and samples were analysed using a BD FACS Symphony A1 (BD Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... and ELISA MAX™ Standard Set Mouse IL-12/IL-23 (p40) (Biolegend) were used to quantify IFNγ production and untethered IL-12 respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... CD19 PE or CD7 PE(304018 Biolegend, 302208 Biolegend, 12-0079-42 Thermofisher) and anti-mouse CD45 eFluor450 (48- 0451-82 ...
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bioRxiv - Genomics 2020Quote: ... Cells were blocked with 1% BSA/PBS for 30 minutes and then incubated with antibodies for HA.11 (BioLegend MMS-101P, 1:2000) and BIP (Abcam ab21685 ...
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bioRxiv - Immunology 2022Quote: ... CD11bhi, F4/80hi) were isolated by FACS using antibodies against CD11b (eBioscience, #11-0112, 1:1600) and F4/80 (Biolegend, #123114, 1:200), and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
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bioRxiv - Immunology 2024Quote: ... and 50,000 cells were added to each well of a 96-well U-bottom plate to provide APC-mediated stimulation alongside soluble 11-CD3 (BioLegend; 0.5 µg/mL) and 11-CD28 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... anti-IgD (follicular B cell marker, Alexa Fluor® 647 anti-mouse IgD antibody, 11-26c.2a, catalog #405708, Biolegend, San Diego, CA), anti-B220 (B cell marker ...
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bioRxiv - Immunology 2022Quote: ... Media was carefully removed and replaced with either rIL-12 (25ng/ml; Biolegend #575402), with or without 2.5μM synthetic cathelicidin ...
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bioRxiv - Zoology 2020Quote: ... IL-8 and IL-12 were gotten from Biolegend (Camino Santa Fe, CA, USA). BCA protein quantitative kit and SYBR Green PCR kit were purchased from Smer Fell Science and Technology Co. ...
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bioRxiv - Cell Biology 2021Quote: ... PAC-1 FITC (1:12, D Bioscience) and annexin V APC (1:60, Biolegend) for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... before staining with IFN-γ (eBioscience, 12-73111-81) and IL-17 (BioLegend, 506904). Data were collected using a BD LSR Fortessa flow cytometer and analyzed using FlowJo Software (Tree Star ...
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bioRxiv - Immunology 2024Quote: ... under Th1-polarizing condition (10 ng/mL recombinant mouse IL-12 (p70) (#577004, BioLegend), and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11 ...
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bioRxiv - Immunology 2023Quote: ... H-2Dd (Anti-mouse H-2Dd antibody; clone 34-2-12; Biolegend; Cat #110606), CD25 (BV510 Rat Anti-Mouse CD25 ...
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bioRxiv - Genomics 2024Quote: ... cells were split into two wells and cultured with either 10ng/mL rhIL-12 (Biolegend), 5ng/mL rhIL-2 ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then incubated with PE anti-IL-12/IL-23 p40 antibody (Biolegend) overnight at 4 °C in 1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... Cytokines were measured by LEGENDPlex™ using a 12-plex Human Th Cytokine Panel (BioLegend).
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bioRxiv - Bioengineering 2021Quote: ... such as the recombinant human matrix metalloproteinases 12 (MMP12, 1 μl, 20 ng/ml, Biolegend, U.S.A), MMP408 (the inhibitor of MMP12 ...
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bioRxiv - Immunology 2021Quote: ... eBioscience Cat#12-1239-42) and CD304 (BV421 anti-human CD304, clone 12C2, BioLegend Cat#354514) (Appendix Figure S1) ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with biotinylated detection antibodies against IL-12 (Biolegend Cat#505302, clone C17.8) and IL-1β (Biolegend Cat#515801 ...
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bioRxiv - Neuroscience 2022Quote: ... 4B12 [a mouse monoclonal antibody that recognises residues 103-108 of human α-synuclein (12)] (BioLegend) at 1:250 and MJFR14 [a rabbit monoclonal antibody that has been reported to recognise assembled human α- synuclein (15)] (Abcam ...
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bioRxiv - Immunology 2023Quote: ... for 3 days at 37°C in the presence of 10 ng/ mL IL-12 (Biolegend).
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bioRxiv - Immunology 2024Quote: The cytokine stimulation conditions were performed with commercially available recombinant IL-12 (100 ng/mL; BioLegend), recombinant IL-18 (100 ng/mL ...
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bioRxiv - Genomics 2021Quote: ... Resultant cell pellets were resuspended in 50ul of antibody cocktail (all antibodies from Biolegend, Supplementary File 12) for 30 minutes before further washing and fixation in 2% paraformaldehyde ...
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bioRxiv - Microbiology 2022Quote: ... 96-well plates were coated with primary capture antibody against IL-12 (Biolegend Cat#511802, clone C18.2) and IL-1β (Biolegend Cat#503502 ...
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bioRxiv - Bioengineering 2022Quote: ... The hybridoma sample was stained with a 12 μg/mL solution of TotalSeqC conjugated anti IgG1 antibody (Biolegend) and incubated for 45 minutes on Ice ...
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bioRxiv - Immunology 2022Quote: Secreted cytokines were assessed with LEGENDplex Human Th Panel 12-plex and analysed with Qognit software (both BioLegend). For cytokine secretion by Tregs ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated with cell activation cocktail (including phorbol 12-myristate 13-acetate and Ionomycin) (BioLegend) in the presence of Golgi Stop (monensin ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Immunology 2023Quote: ... cells were treated with 81 nM phorbol 12-myristate 13-acetate (PMA) plus 1.34 µM Ionomycin (BioLegend, 423301) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Cytokines in the cell culture supernatants were quantified using Human Th Cytokine Panel (12-plex) (Cat. 741027, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... were stimulated with 500x Cell Activation Cocktail containing 40.5 μM phorbol-12-myristate 13-acetate (PMA) and 669.3 μM ionomycin (Biolegend) in the presence of 5 μg/mL BFA (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Naïve T cells were skewed to the following subsets with cytokines: Th1 (10ng/mL mIL-12 (Biolegend, 577004), 10ng/mL recombinant human IL-2 ...
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bioRxiv - Immunology 2022Quote: ... BMDMs supernatant and intracellular IL-12p70 were quantified by using ELISA MAXTM Deluxe Set Mouse IL-12 (p70) (Biolegend).
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Molecular Biology 2023Quote: ... Human IL-2 ELISA MAX Deluxe kit and Human IL-12 (p70) ELISA MAX Deluxe kit in accordance with the manufacturer’s instructions (BioLegend).