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251 - 300 of 8452 citations for 4 3 2 Chloroethyl 3 nitrosoureido tetrahydro 2H thiopyran 1 1 dioxide since 2020

• Cell-Intrinsic and Extrinsic Effects of Galectin-1 and Galectin-3 in B-Cell Precursor Acute Lymphoblastic Leukemia

  Fei Fei, et al., bioRxiv - Cancer Biology 2021

  Quote: ... cells were incubated with PE-Galectin-3 (Biolegend cat# 126706) or matched isotype control for 15 mins and analyzed on BD Accuri C6 cytometer (BD Biosciences ...
• Tissue-resident regulatory T cells exert dualistic anti-tumour and pro-repair function in the exocrine pancreas

  Julie Stockis, et al., bioRxiv - Immunology 2024

  Quote: ... injection of 3 µg anti-CD45.2-PE (clone 104, Biolegend) antibody 3 min prior to sacrifice ...
• Early infection response of the first trimester human placenta at single-cell scale

  Regina Hoo, et al., bioRxiv - Genomics 2023

  Quote: ... TotalSeq™-B0253 anti-human Hashtag 3 Antibody (394635, Biolegend), TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637 ...
• Targeting CXCR4 with [²¹²Pb/²⁰³Pb]-Pentixather Significantly Increases Overall Survival in Small Cell Lung Cancer

  Keegan A. Christensen, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 3×106 cells were stained with Zombie Aqua (423102, BioLegend) for 20 min at room temperature for live/dead cells discrimination ...
• Gene-corrected Parkinson’s disease neurons show the A30P alpha-synuclein point mutation leads to reduced neuronal branching and function

  Peter A. Barbuti, et al., bioRxiv - Neuroscience 2020

  Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
• Dynamically stiffening biomaterials reveal age- and sex-specific differences in pulmonary arterial adventitial fibroblast activation

  Mikala C. Mueller, et al., bioRxiv - Bioengineering 2024

  Quote: ... 4′,6-diamidino-2-phenylindole (DAPI; BioLegend) was diluted 1:10,000 from a 5 mg mL-1 stock solution in deionized water ...
• Therapeutic Interruption of T Cell Development Generates High-Affinity T Cells That Escape Exhaustion and Improve Cancer Immunotherapy

  Erica Dhuey, et al., bioRxiv - Immunology 2022

  Quote: ... TotalSeq™-C anti-mouse Hashtag Antibodies 1-4 (BioLegend) were used to differentiate between tumor samples.
• Recombinant T cell receptors specific for HLA-A*02:01-restricted neoepitopes containing KRAS codon 12 hotspot mutations

  Craig M. Rive, et al., bioRxiv - Immunology 2020

  Quote: ... CD8-FITC and 4-1BB-CD137 APC (1/100, BioLegend). Cells were incubated at 37°C for 10 min ...
• A Novel MHC-Independent Mechanism of Tumor Cell Killing by CD8⁺ T Cells

  Emily Lerner, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse aLFA-1 (Clone M17/4, Biolegend Catalogue # 101118), or anti-human NKG2D (Clone 1D11 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-IL-4 (1:100 dilution; clone 11B11, BioLegend). Cells were then washed twice with 1x Perm buffer and fixed with BD Cytofix for 10 min at room temp ...
• Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC

  Luyao Ao, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Panel 4: APC/750-conjugated CD45 (1:100, 147713, Biolegend), BV421-conjugated CD3 (1:100 ...
• NR4A3 deficiency ameliorates contact hypersensitivity and exacerbates psoriasis by regulating gene expression in dendritic cells

  Mayuka Katagiri, et al., bioRxiv - Immunology 2024

  Quote: ... PE anti-mouse IL-4 (clone 11B11, BioLegend, 1:100), PE anti-mouse IL-17A (TC11-18H10.1 ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... and IL-4 (20 ng ml−1, BioLegend Cat# 574004) (Mo-DCs ...
• Spermidine/spermine N1-acetyltransferase controls tissue-specific regulatory T cell function in chronic inflammation

  Teresa Neuwirth, et al., bioRxiv - Immunology 2024

  Quote: ... anti-4-1BBL PE-Cy7 (Biolegend, Cat: 311511, 1:300), and anti-CD155 AF647 (Biolegend ...
• Two distinct durable human class-switched memory B cell populations are induced by vaccination and infection

  Cory A. Perugino, et al., bioRxiv - Immunology 2024

  Quote: ... anti-human-CD38 (BioLegend, Clone HIT2, BV421, 1:100, 4℃); anti-human-CD21 (BD Biosciences ...
• Computationally designed mRNA-launched protein nanoparticle vaccines

  Grace G. Hendricks, et al., bioRxiv - Immunology 2024

  Quote: ... and anti-IL-4 (PerCPCy5.5; BioLegend #504124, 1:100 dilution). Cells were analyzed with an LSRII.UV analyzer at the Stanford Shared FACS Facility (SSFF).
• HOIL1 regulates group 3 innate lymphoid cell numbers in the colon and protects against systemic dissemination, colonic ulceration, and lethality from Citrobacter rodentium infection

  Victoria L. Hartley, et al., bioRxiv - Immunology 2023

  Quote: ... The cells were resuspended in 1 ml R10 and 1-2 million cells were plated in 1ml R10 with 1 μl Brefeldin A (BioLegend) for 4 hours ...
• The role of B cells in immune cell activation in polycystic ovary syndrome

  Angelo Ascani, et al., bioRxiv - Immunology 2023

  Quote: ... CD138-PE/Cyanine7 (Syndecan-1, clone 281-2, BioLegend), CD27-APC (clone LG.3A10 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... IL-2 (1:100 dilution; clone JES6-5H4, BioLegend) and anti-IL-4 (1:100 dilution ...
• Extrafollicular responses are sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus

  Lasse F. Voss, et al., bioRxiv - Immunology 2022

  Quote: ... CD138-PE clone 281-2 (142504, BioLegend, 1:500), CD169-PE clone 3D6.112 (142404 ...
• Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC

  Luyao Ao, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Panel 2: BV421-conjugated CD45 (1:100, 103133, Biolegend), PE/Cy7-conjugated CD3 (1:100 ...
• Opposing diet, microbiome and metabolite mechanisms regulate inflammatory bowel disease in a genetically susceptible host

  Gabriel Vasconcelos Pereira, et al., bioRxiv - Microbiology 2023

  Quote: ... APC-conjugated anti-CD138 (Biolegend, 281-2; 1/100), FITC-conjugated anti-IgA (eBiosciences ...
• Spermidine/spermine N1-acetyltransferase controls tissue-specific regulatory T cell function in chronic inflammation

  Teresa Neuwirth, et al., bioRxiv - Immunology 2024

  Quote: ... anti-IL-2 PE (Biolegend, Cat: 340450, 1:50), anti-IL-17A APC (eBioscience ...
• The extrafollicular B cell response is a hallmark of childhood idiopathic nephrotic syndrome

  Tho-Alfakar Al-Aubodah, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human IgD BV510 (1:20, IA6-2, BioLegend), anti-human IgM Alexa Fluor 488 (1:40 ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD138 BV785 (1:80, clone 281-2, BioLegend) for 30 min on ice and then washed once in FACS buffer ...
• CD38 regulates ovarian function and fecundity via NAD⁺ metabolism

  Rosalba Perrone, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CD138-BV785 (BioLegend, clone 281-2, 142534, 1:800), CD38-Pac Blue (BioLegend ...
• Computationally designed mRNA-launched protein nanoparticle vaccines

  Grace G. Hendricks, et al., bioRxiv - Immunology 2024

  Quote: ... anti-IL-2 (PE; BioLegend #503808, 1:100 dilution), and anti-IL-4 (PerCPCy5.5 ...
• Prostacyclin as a Negative Regulator of Angiogenesis in the Neurovasculature

  Tasha R Womack, et al., bioRxiv - Neuroscience 2021

  Quote: ... Rat antibody against Notch 3 intracellular domain was obtained from Biolegend. Blots were probed with a mouse anti-actin (ThermoFisher ...
• Intracellular sterol sensing controls intestinal B cell differentiation

  Bruno C. Trindade, et al., bioRxiv - Immunology 2021

  Quote: ... Color development was stopped with 3 M H2SO4 stop solution (Biolegend). Purified mouse IgA (Southern Biotech ...
• The Ebola virus matrix protein clusters phosphatidylserine, a critical step in viral budding

  Monica L. Husby, et al., bioRxiv - Microbiology 2022

  Quote: ... Cells were blocked with 3% BSA/PBS cell staining buffer (BioLegend) and incubated at 37°C for 2 hours ...
• The challenge of distinguishing cell-cell complexes from singlet cells in non-imaging flow cytometry and single-cell sorting

  Julie G Burel, et al., bioRxiv - Immunology 2020

  Quote: ... 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
• Mitochondrial respiration contributes to the interferon gamma response in antigen presenting cells

  Michael C. Kiritsy, et al., bioRxiv - Immunology 2020

  Quote: ... CD40 (APC anti-mouse CD40 Antibody, Clone 3/23 Biolegend 124611), or PD-L1 (APC anti-mouse CD274 (B7-H1 ...
• Profound Treg perturbations correlate with COVID-19 severity

  Silvia Galván-Peña, et al., bioRxiv - Immunology 2020

  Quote: ... CD25 PE-Cy7 (clone BC96, BioLegend, cat# 302611, dilution 3:100); KLRG1 APC-Cy7 (clone 2FI/KLRG1 ...
• A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems

  Paulina M Wysmolek, et al., bioRxiv - Neuroscience 2022

  Quote: ... and anti-Tubulin ß 3 antibody (Clone Tuj1, BioLegend, MMS-435P) respectively.
• Inhibition of Acyl-CoA Synthetase Long Chain Isozymes Decreases Multiple Myeloma Cell Proliferation and Causes Mitochondrial Dysfunction

  Connor S. Murphy, et al., bioRxiv - Cancer Biology 2024

  Quote: ... washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201) and stained with APC-Annexin V (1:20 ...
• ZEB2 regulates the development of CD11c+ atypical B cells

  Xin Gao, et al., bioRxiv - Immunology 2022

  Quote: ... in the presence of 3 μg/ml anti-CD40 antibody (Biolegend) w/wo 50 ng/mL IL-21 ...
• The cis-regulatory logic integrating spatial and temporal patterning in the vertebrate neural tube

  Isabel Zhang, et al., bioRxiv - Developmental Biology 2024

  Quote: ... 3 µM CHIR99021 (Axon) and 10 ng/ml mouse LIF (BioLegend)) ...
• Identification of small molecule enhancers of NK cell tumoricidal activity via a tumor microenvironment-mimicking co-culture assay

  Aylin Binici, et al., bioRxiv - Immunology 2024

  Quote: ... and TIM-3(F38-2E2)-APC-Fire 750 (BioLegend (San Diego)) ...
• Flotillin-2 dampens T cell antigen-sensitivity and functionality

  Sookjin Moon, et al., bioRxiv - Immunology 2024

  Quote: ... and TotalSeq™-A0303 anti-mouse Hashtag 3 Antibody (Biolegend, 155805).
• A genome-wide genetic screen uncovers novel determinants of human pigmentation

  Vivek K. Bajpai, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse TYRP1 (MEL-5) (1:500; 4°C overnight; BioLegend, 917801), rabbit MITF (1:200 ...
• Vitamin A-treated natural killer cells reduce interferon-gamma production and support regulatory T cell differentiation

  Mingeum Jeong, et al., bioRxiv - Immunology 2022

  Quote: ... 1 µg/ml anti-IL-4 (clone 11B11) (all from Biolegend), 20 ng/ml IL-6 ...
• Vitamin A-treated natural killer cells reduce interferon-gamma production and support regulatory T cell differentiation

  Mingeum Jeong, et al., bioRxiv - Immunology 2022

  Quote: ... 1 µg/ml anti-IL-4 (clone 11B11) (both from Biolegend), 30 U/ml rhIL-2 (NIH) ...
• T:B cell cooperation in ectopic lymphoid follicles propagates CNS autoimmunity

  Anna Kolz, et al., bioRxiv - Immunology 2023

  Quote: ... 4 % goat serum and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT in a humidified dark chamber ...
• βIII-Tubulin is a Brake on Extrinsic Cell-Death in Pancreatic Cancer

  John Kokkinos, et al., bioRxiv - Cancer Biology 2022

  Quote: ... βIII-tubulin (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and α-smooth muscle actin (αSMA ...
• Distinct sets of molecular characteristics define tumor-rejecting neoantigens

  Anngela C. Adams, et al., bioRxiv - Immunology 2024

  Quote: ... CD4-APC (1:80; clone RM4-4; BioLegend Cat. No. 116013), CD4-BV421 (1:160 ...
• Distinct sets of molecular characteristics define tumor-rejecting neoantigens

  Anngela C. Adams, et al., bioRxiv - Immunology 2024

  Quote: ... CD4-BV421 (1:160; clone RM4-4; BioLegend Cat. No. 116023), FoxP3-APC (1:20 ...
• Heterotypic responses against nsp12/nsp13 from prior SARS-CoV-2 infection associates with lower subsequent endemic coronavirus incidence

  David J. Bean, et al., bioRxiv - Immunology 2023

  Quote: ... PE anti-human CD137 (4-1BB (1:100 dilution, BioLegend, 309803). After staining ...
• Two distinct durable human class-switched memory B cell populations are induced by vaccination and infection

  Cory A. Perugino, et al., bioRxiv - Immunology 2024

  Quote: ... anti-human CD19 (BioLegend, Clone SJ25C1, APC-Cy7, 1:25, 4℃); anti-human CD27 (BD Biosciences ...
• Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

  Gerdien Mijnheer, et al., bioRxiv - Immunology 2022

  Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
• Spatial transcriptomics reveals molecular dysfunction associated with Lewy pathology

  Thomas Goralski, et al., bioRxiv - Neuroscience 2023

  Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250, pS129 α-synuclein (81A, Biolegend 825701, 1:1000).