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Citations for BioLegend :
2901 - 2950 of 9629 citations for Human Glutamine Fructose 6 Phosphate Transaminase 1 GFPT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Fc block was performed with a combination of Fc block -Human Tru Stain Fc block (Catalogue No. 422301, Biolegend), Nova Block Solution ...
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bioRxiv - Immunology 2022Quote: After thawing cells were washed and Fc receptors were blocked with TruStain FcX (mouse or human as appropriate; Biolegend) for 5 minutes at room temperature before staining with diluted antibodies (Supplemental Tables 2 ...
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bioRxiv - Immunology 2022Quote: All surface staining was done in the presence of Human Fc block and Monocyte Blocker (Biolegend, San Diego, CA). Following staining ...
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bioRxiv - Immunology 2022Quote: ... low endotoxin (Super affinity) (Aero) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend). For cytotoxicity assay analysis ...
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bioRxiv - Immunology 2022Quote: ... Cells were spun down and supernatant was frozen for Legendplex analysis (LEGENDplex Human CD8/NK Panel 13-plex, BioLegend, according to the manufacturer’s information) ...
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bioRxiv - Cancer Biology 2022Quote: ... and genotyped for HLA*A:02 via flow cytometry with purified anti-human HLA-A2 antibody clone BB7.2 (BioLegend). HLA-A*02 positive samples were selected and dendritic cells were generated via plate adherence and pulsed with mHAs that are not endogenous to the sample (Peptide 2.0 Inc) ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were stained with live dye (Calcein AM) and PE anti-human CD45 antibody (BioLegend, cat no 368510) mixture ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Immunology 2024Quote: ... These fixed and permeabilized cells were further stained with mouse anti-human IFN-γ antibody conjugated with PE (BioLegend) and Brilliant Violet 421 conjugated mouse anti-human TNF antibody (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Immunology 2024Quote: ... Human CD4+ T cells (4X105/well in 96-well plate) were activated with plate-coated αCD3 (5µg/ml, Biolegend) and αCD28 (10µg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100,000 IFNγ-treated tumor cells were seeded together with 200,000 pTc or TiTc per well in Tc medium containing anti-human CD107a-FITC (Biolegend). After 1h incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated for 3 days using 3 μg anti-human CD3 and CD28 antibodies (Biolegend, 300438, 377604) and 50 ng/mL of IL-2 (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... single cell suspensions were incubated for 15 minutes at room temperature in the dark with Human TruStain FcX (Biolegend) at a concentration of 5µL per million cells to block unspecific Fc Receptor binding ...
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bioRxiv - Immunology 2023Quote: ... CD141 BV605(Clone:M80 Cat:344117) in the presence of human anti-Fc Receptor antibodies (TrueStain; Biolegend; cat. no. 101320). cDCs1 were FACS-sorted as CD45+Lin- (Lin:CD3 ...
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bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human TCR α/β antibody (IP26: Biolegend), HLA.A2/9V-AF647 or CD19-AF647 to determine the concentration of the staining agent leading to label saturation for CMV-specific RA14 TCR T-cells ...
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bioRxiv - Immunology 2023Quote: ... BSA/0.01% (v/v) Tween-20 in cold RNase-free PBS + 10 μl Human TruStain FcX blocking solution (#422301, BioLegend)) and incubated for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Live naïve CD4+ T cells were purified from PBMCs post staining with fluorescence labelled antibodies to human CD3(OKT3, Biolegend), CD4 (RPA-T4 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... For surface antigen staining 20 min incubation with the Alexa Fluor 488 anti-human CD29 (Biolegend, clone TS2/16), PE anti-human CD184[CXCR4] (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Then cells were resuspended in Maxpar® cell staining buffer (Fluidigm) and blocked with Human TruStain FcX™(Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... aureus Newman (from 2.106 to 2.104/well) in the presence of anti-human CD107a-AF647 mAb (clone H4B4, Biolegend, France) added at the beginning of stimulation in serum and antibiotic free RPMI media in 96 well round bottom plates ...
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bioRxiv - Immunology 2023Quote: ... Each B cell sample was split into two aliquots of at least 500,000 cells for labeling with different TotalSeqC anti-human cell hashing antibodies (BioLegend). Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated overnight at 4 °C on a tube rotator at 18 rpm in 800 μL of PBS/0.2% BSA containing 15 nM human IgG1 (BioLegend) that had been conjugated with Alexa647 using NHS chemistry (Molecular Probes ...
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bioRxiv - Immunology 2024Quote: To measure levels of 13 soluble factors simultaneously using the LEGENDplex Human CD8/NK Panel (BioLegend, San Diego, USA), supernatants were collected from cytotoxicity assays conducted in the Maestro Z instrument at endpoint and stored at -20°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and live cells stained with APC anti-human GD2 (#357306; Biolegend) (isotype control, APC Mouse IgG2a, k; #400220, Biolegend) or Anti-human/mouse CD49f antibody (#313620 ...
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bioRxiv - Cancer Biology 2024Quote: ... Pluripotency marker expression was assessed every 10 passages by flow cytometry (PE anti-human SSEA-4 antibody, BioLegend, #330405) or RT-qPCR for DNMT3B ...
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bioRxiv - Bioengineering 2024Quote: ... Cells from each sample were labeled with TotalSeq™-B0251-0254 anti-human Hashtag Antibodies (clone LNH-94, Biolegend, RRID ...
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bioRxiv - Immunology 2024Quote: Up to 20,000 cells per sample (Table S1) were stained with the TotalSeq-C Human Universal Cocktail v1 (BioLegend) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... in presence (stimulated condition) or absence (unstimulated condition) of 5 μg/ml anti-human CD3ε (clone OKT3; #317302, BioLegend) in PBS at 37°C for 2h ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-c-Kit (BioLegend), anti-Sca1 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... c-kit (2B8, Biolegend), CD135 (A2F10 ...
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bioRxiv - Cell Biology 2024Quote: ... c-Kit (ACK2, Biolegend), GP1bβ (Emfret ...
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bioRxiv - Genetics 2024Quote: ... KIT-PE (Biolegend 313204), FOLH1-APC (Biolegend 342508) ...
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Microbiology 2022Quote: ... unpulsed splenocytes and the S-peptide-pulsed splenocytes were stained with 0.5 μM and 5 μM (respectively) of 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE; BioLegend, San Diego, CA, USA). The unpulsed and peptide-pulsed splenocytes were mixed at a 1:1 ratio (5 × 106 cells each) ...
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bioRxiv - Immunology 2021Quote: Serum samples were collected three hours after the first STING agonist injection and analyzed for inflammatory cytokines (IFN-α, IFN-ß, TNF-α and IL-6) using a LEGENDplex Mouse Inflammation Panel (BioLegend). For cell culture supernatants ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were subsequently blocked in flow buffer supplemented with either anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... flow buffer was supplemented either with anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend). The following antibodies were used for flow analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Immunology 2021Quote: ... or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus, referred to as anti-h/pCD47; Biolegend). The level of cell surface CD47 is expressed as median fluorescent intensity (MFI) ...