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Citations for BioLegend :
2901 - 2950 of 3905 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The antibodies used were as follows: allophycocyanin (APC)-labeled anti-mouse Fms (AFS98) and APC-labeled anti-human Fms (9-4D2-1E4) (both from BioLegend).
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bioRxiv - Cancer Biology 2024Quote: ... quantifying the ratio of human CD45 (labelled with PE) to mouse CD45 (labelled with APC-Cy7) all of which were supplied by Biolegend.
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for CXCR5 expression for 15 minutes at 37C by adding 30μL of anti-human CXCR5 AlexaFluor 488 (Biolegend; 356912) to dry pellet ...
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bioRxiv - Immunology 2024Quote: ... AML cell lines and samples from in vivo studies were blocked with Human TruStain FcX (BioLegend, San Diego, CA, USA) prior to staining ...
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bioRxiv - Immunology 2024Quote: ... Fcγ receptors were blocked prior to surface antigen staining by incubating the cells in flow cytometry staining buffer containing Human TruStain FcX antibody (BioLegend) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... Whole blood was immediately stained for flow cytometric analysis with combinations of the following human specific antibodies: CD4 (BV421, RPA-T4,Biolegend), CD4 (APC-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for flow cytometry analysis with the following human specific antibodies: HLA-A,B,C(PE-Cyanine7, W6/32, Biolegend), CD4 (BV421 ...
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bioRxiv - Immunology 2024Quote: ... Isolation purity was verified by flow cytometry using antibodies against neutrophil surface markers anti-human CD11b (Alexa Fluor 594) (Biolegend) and anti-human CD15 (SSEA-1)(Alexa Fluor 488 ...
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bioRxiv - Immunology 2024Quote: ... human PBMCs were preincubated for 30 min with 2 μg/mL anti-human IL-12 (clone 24910; BioTechne) or 2 μg/mL mouse IgG1 (clone MOPC-21; BioLegend). In functional experiments ...
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bioRxiv - Immunology 2024Quote: Human bone marrow mononuclear cells (1,5 Million cells per stain) were first incubated with Fc Receptor blocking solution Human TruStain FcX™ (Biolegend) and then stained extracellularly for 20 minutes at 4°C with anti-CD38-APC-antibody ...
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bioRxiv - Immunology 2024Quote: Cytokine levels were measured from culture media supernatants harvested 24 hours after plating using LegendPlexTM Human Inflammation Panel 1 (Biolegend), per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... peptide-pulsed K562/HLA-E cells were stained with APC-labeled anti-human HLA-E mAb (clone 3D12, BioLegend, USA). Then the CD94/NKG2A+HLA-E+ and CD94/NKG2C+HLA-E+ K562/HLA-E cells were gated as targeted cells.
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Bioengineering 2024Quote: ... Surface staining was carried out using a fluorochrome-labeled PE-conjugated anti-human CD11a (HI111) monoclonal antibody obtained from BioLegend.
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were analyzed for STn expression by recombinantly expressed anti-STn scFv-Fc and anti-human IgG-AF647 (Biolegend).
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bioRxiv - Cell Biology 2024Quote: ... media was removed from cells and 45 µl of EMEM+1/100 Human TruStain FcX blocking solution (Fc Block, BioLegend) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... at a dilution of 1:5000 followed by Fc receptor blocking using a combination of 0.5 µL per test of Purified Anti-Mouse CD16/CD32 Fc block (Tonbo) and 1 µL per test of Human Trustain FcX (Biolegend). Samples and fluorescence minus one controls were stained using antibodies at the dilutions listed in Table S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 – 1 x 105 cells were washed with CSM and incubated with 100 µl antibody mix (1 µl APC anti-human CD19 (clone: HIB19, BioLegend); or 1 µl APC anti-human CD22 (clone ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed twice with CSM and incubated with 1 µl Pacific Blue anti-human CD19 antibody (clone: HIB19, BioLegend) in 100 µl CSM at RT for 10 min ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained for 30 mins at 4°C in the dark with anti-human antibodies specific for anti-CD14 [APC/Cyanine-7] (301819, BioLegend), anti-CD16 [Fluorescein isothiocyanate (FITC)] (360715 ...
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bioRxiv - Immunology 2021Quote: ... using the LegendPlex kit (BioLegend).
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bioRxiv - Immunology 2021Quote: ... using the LegendPlex kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... c-kit-BV605 (104D2; BioLegend), CRTH2-PE (MACS Milltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... permeabilized (BioLegend cytokine staining kit) and stained with anti-cytokine antibodies.
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA ...
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bioRxiv - Cell Biology 2021Quote: ... Then 1×106 cells were washed again with 1x Hanks buffer and stained with 5µl of Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) Antibody (Biolegend#125608) in100µl BD Pharmingen™ Stain Buffer (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies: anti-human CD45-APC (Biolegend, 368512), anti-human CD3-Pacific Blue (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...
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bioRxiv - Genomics 2020Quote: ... and resuspended in a solution of FACS buffer with a 1:100 dilution of PE anti-human B2-microglobin (Biolegend 316306). Cells were incubated for 30 mins in the dark at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... The cells were then stained with PE-conjugated anti-Human IgG Fc antibody (clone M1310G05, BioLegend 410720, San Diego, CA, USA) to detect bound anti-CTLA-4 and with fluorescein isothiocyanate (FITC)-conjugated anti-CD27 (clone O323,
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bioRxiv - Bioengineering 2020Quote: ... cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-cMyc antibody and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with APC-conjugated anti-human HLA antibody (clone W6-32; cat. no. 311410; BioLegend, San Diego, CA). Cells were subsequently analyzed by flow cytometry for GFP cells/DsRed cells (sensitive/resistant ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... CD81 expression was measured 7 days post-transduction by dislodging cells with TrypLE and live cells were stained with APC-conjugated anti-human CD81 antibody (Biolegend 349509). CD81 expression was assessed on a BD LSRII with >90% of Calu3-CRISPRi cells with CD81 knocked down compared to a non-targeting sgRNA control.
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bioRxiv - Molecular Biology 2021Quote: ... incubated with an Alexa Fluor 647-labeled mouse monoclonal antibody against the human IgG1-Fc (Biolegend, San Diego, USA, cat #409320) and analyzed in a Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... and Zombie Dye Violet Blue (Biolgend; 423114) for 15 minutes at 4degC followed by surface labeling with PE anti-human CD45 (Biolegend; 304008) for 30 minutes at 4degC ...
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bioRxiv - Immunology 2022Quote: ... the plate was washed five times with PBST and incubated with anti-mouse secondary antibody with minimal cross reactivity with human IgG (Biolegend, 405306). The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.1% BSA/PBS 15 min on ice) and incubated with either FITC-anti-human CD3 (clone HIT3a; BioLegend, San Diego, CA) and PE-anti-human CD56/NCAM (HCD56 ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... Annexin V-APC (e-biosciences, San Diego, CA) and with cell surface markers α-human CD4 PE (Biolegend, San Diego, CA), α-human CD3 PerCP (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: The cytokines in adipocytes supernatant were quantified by a bead-based multiplex assay (LEGENDplexTM Human Panel; BioLegend, San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Immunology 2020Quote: ... TnT cells and Jurkat-derived cell lines were stimulated overnight with either 10 μg ml−1 plate-bound anti-human CD3e antibody (clone OKT3, BioLegend, #317326) or 1X eBioscience Cell Stimulation Cocktail (81 nM PMA ...
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bioRxiv - Cell Biology 2020Quote: ... The antibodies used for flow cytometry were as follows: PE-conjugated mouse anti-human CD29 (Biolegend, San Diego, CA, USA; 303004), PE-conjugated mouse anti-human CD73 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... in the presence or absence of human IFN-β (500 U/ml: Pestka Biomedical Laboratories) or IL-27 (100 ng/ml: BioLegend) without adding IL-2.
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bioRxiv - Genetics 2021Quote: ... A antibody panel was used to identify and sort the CD3+CD8+ T cell population: Human TrueStain FcX (Biolegend; Cat# 422302), PE anti-human CD45 (Biolegend ...