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Citations for BioLegend :
2851 - 2898 of 2898 citations for Recombinant Human Interleukin 17 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Apical supernatant from cells at each harvest time point was assessed using LEGENDPlex Human Anti-Virus Response Panel (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions and using a FACSCanto II flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Samples were stained immediately after fixation with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with PBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend, Catalog #101207, 1:50) was added to measure and gate on differentiated HL-60 cells ...
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bioRxiv - Bioengineering 2023Quote: ... coated glass bottom chamber and then labeled with 1ugꞏml−1 of Alexa Flour (AF) 488 conjugated anti-human CD45 antibodies (Biolegend, Cat# 304017, clone HI30) in 2% BSA in PBS for 20 min at RT ...
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bioRxiv - Immunology 2023Quote: ... prior to the end of the CD45- sort one vial of TotalSeq-C Human Universal Antibody Cocktail v1.0 (Biolegend, San Diego, CA, USA) was equilibrated to room temperature for 5min and then spun at 10,000g for 30 seconds ...
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bioRxiv - Cancer Biology 2023Quote: ... dendritic cell markers were purchased for characterization of immune cells using flow cytometry: PerCP/Cy5.5 anti-human CD3 and CD11c (Biolegend, San Diego, CA, USA), PE anti-human CD4 and CD56 (Biolegend) ...
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bioRxiv - Biophysics 2023Quote: ... Flow cytometry is further used to determine the expression profile of the integrins CD29 (PE anti-human CD29 antibody; Biolegend, cat. no. 303003) and CD18 (PE anti-human CD18 antibody ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the NK-92 staining panel: anti-CD107a (LAMP-1) FITC (BioLegend catalog #328606, clone H4A3), anti-CD56 (NCAM ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for T cells: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD26 PerCP/Cy5.5 (BioLegend catalog #356112 ...
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bioRxiv - Immunology 2024Quote: ... and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3, BioLegend, cat. 317325) and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2 ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Cancer Biology 2022Quote: ... was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (Biolegend, San Diego, CA, USA). Mice were monitored closely to detect disease onset and treatment started when hCD45+ cells were detectable in the peripheral blood ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were used for these studies: anti-human CD47-APC (1µl/106 cells in 100µl; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3), anti-human CD47-FITC (5µl/106 cells in 100µl ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were blocked with 0.5 % BSA for 10 min on ice and incubated with the primary mAbs biotin-anti-human CD274 (B7-H1, PD-L1) (clone 29E.2A3, BioLegend, San Diego, CA, USA) or biotin anti-mouse CD274 (clone 10F.9G2 ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Immunology 2024Quote: ... in the presence or absence of rIFNB (500 ng/mL human [Peprotech, Cranbury, NJ, US]; 50 ng/mL murine [BioLegend, San Diego, CA, US]), with or without neutralizing antibodies or EGTA ...
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bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
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bioRxiv - Immunology 2024Quote: ... Isolated CD4+ T-cells were either maintained in a naïve state by seeding in complete growth medium alone or underwent TH1-activation in tissue culture flasks pre-coated with anti-human CD3 antibody (10 µg/mL) (BioLegend, San Diego, CA, US) in complete medium supplemented with anti-human CD28 antibody (5 µg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 x with 1% BSA before incubating for 1 h in anti-human IgG secondary antibody labelled with either APC (BioLegend, #410712, dilution 1:100) or Phycoerythrin (Abcam ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Immunology 2024Quote: ... Blocking of CD47 was performed with purified mouse anti-human CD47 mAb (αCD47-fuFc) clone CC2C6 (cat. no. 323102, Biolegend, San Diego, CA, USA), and humanized anti-CD47 IgG2σ mAb (αCD47-siFc ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were spun down to remove cell debris and frozen at -80 °C until analysis using the ELISA MAX™ Deluxe Set Human IFN-γ (BioLegend catalog #430104) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... mice were intravenously injected with 15 μg of mouse anti-human CD31 coupled to Alexa-Fluor-647 (clone WM59, Biolegend, San Diego, CA, United States) approximately 2 hrs prior to imaging.
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... spleen and bone marrow were isolated and evaluated for the percentage of human CD45+ cells by flow cytometry (CD45-PerCP-Cy5.5, Biolegend Cat: 304028; BD FACS Canto II). Populations were defined and analyzed using FlowJo software (Flowjo v10.7.1 ...
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bioRxiv - Immunology 2022Quote: The following human cytokines were assessed by ELISA as previously described (10): human IFN-γ (capture MD-1 mAb; revealing biotinylated 4S.B3 mAb, Biolegend Cat#507502 and 502504, respectively), human IL-13 (capture JES10-5A2 mAb ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% Tween-20 and incubated overnight at 4°C in a humidified chamber with primary human anti-CD9 (Biolegend, catalog no. 312102, dilution 1:1000), human anti-CD63 (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... The bone marrow was harvested from each mouse and the infiltrated CCRF cells were identified by staining for a human CD45 antibody (Biolegend; AB_2687375; BLG-368522; 1:200).
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bioRxiv - Systems Biology 2023Quote: ... the four-class iPSC dataset (Cell, Mitotic, Dying cell, Debris) was stained with PE anti-human TRA-1-81 (1:300, Biolegend, #330708, clone TRA-1-81), while the five-class iPSC morphology dataset was stained with Alexa Fluor ® 555 anti-GSK3 beta (1:300 ...
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bioRxiv - Bioengineering 2023Quote: ... we collected the supernatants and quantified the concentrations of each cytokine using the LEGENDplex™ Human Essential Immune Response Panel (13-plex) (BioLegend, San Diego, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Immunology 2023Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend, 343606 at 1.25 µg/mL).
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bioRxiv - Immunology 2023Quote: ... and then stained for 20 minutes at 4 °C using the following antibodies: Brilliant Violet 605™ anti-human interferon-gamma (IFN-γ) (506542, Biolegend; 1 μL/well). After being stained ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for B cells: anti-CD19 Alexa Fluor® 488 (BioLegend catalog #302219, clone HIB19), anti-CD3 PerCP/Cy5.5 (TONBO catalog #65-0037-T100 ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for NK cells: (donors 1-2) anti-IFN-γ FITC (BioLegend catalog #506504, clone B27), anti-CD19 PerCP/Cy5.5 (BioLegend catalog #309504 ...
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bioRxiv - Biochemistry 2020Quote: ... the cultured cells were harvested and incubated with either fluorescein isothiocyanate (FITC)-conjugated anti-human FR-β antibody (m90916, a gift from Philip S. Low) or allophycocyanin (APC)-conjugated anti-human FR-β antibody (mouse IgG2a, BioLegend, San Diego, CA, USA), and isotype controls (mouse IgG-FITC ...
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bioRxiv - Genomics 2019Quote: ... cells were washed once in PBS and once in Flow buffer (PBS with 2% BSA) and stained with anti-human CD47-APC antibody (clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3) in Flow buffer (20µl antibody/40×106 cells/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation with primary antibodies (rat anti-human α-synuclein, 15G7, 1:100 Enzo Life Sciences; mouse anti-α-synuclein [Syn1, clone 4D6], 1:1000, 834301, BioLegend; rabbit anti-GAPDH 1:2000 (D16H11) Cell Signaling Technologies ...