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Citations for BioLegend :
2851 - 2900 of 10000+ citations for Anti CD25 SAP human Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: To study the human HSPC phenotype of the gene edited cells were stained with hCD34-Pecy7 (581 clone, Biolegend), hCD38-PE (HB7 clone ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2022Quote: ... Fc block was performed with a combination of Fc block -Human Tru Stain Fc block (Catalogue No. 422301, Biolegend), Nova Block Solution ...
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bioRxiv - Immunology 2022Quote: After thawing cells were washed and Fc receptors were blocked with TruStain FcX (mouse or human as appropriate; Biolegend) for 5 minutes at room temperature before staining with diluted antibodies (Supplemental Tables 2 ...
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bioRxiv - Immunology 2022Quote: All surface staining was done in the presence of Human Fc block and Monocyte Blocker (Biolegend, San Diego, CA). Following staining ...
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bioRxiv - Immunology 2022Quote: ... Cells were spun down and supernatant was frozen for Legendplex analysis (LEGENDplex Human CD8/NK Panel 13-plex, BioLegend, according to the manufacturer’s information) ...
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bioRxiv - Microbiology 2024Quote: ... and IL-1β concentrations were determined with the ELISA MAX™ Deluxe Set Human IL-1β (BioLegend, Cat# 437004) in 96-well half area Costar assay plates (Corning ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Immunology 2024Quote: ... Human CD4+ T cells (4X105/well in 96-well plate) were activated with plate-coated αCD3 (5µg/ml, Biolegend) and αCD28 (10µg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... single cell suspensions were incubated for 15 minutes at room temperature in the dark with Human TruStain FcX (Biolegend) at a concentration of 5µL per million cells to block unspecific Fc Receptor binding ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...
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bioRxiv - Immunology 2023Quote: ... BSA/0.01% (v/v) Tween-20 in cold RNase-free PBS + 10 μl Human TruStain FcX blocking solution (#422301, BioLegend)) and incubated for 10 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 1-16 Antibody (1:000, Clone 6E10, N-terminal, a.a. 1-16, human specific only, 803014 BioLegend, CA, USA); β-Amyloid ...
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bioRxiv - Neuroscience 2023Quote: ... 17-24 Antibody (1:1000, Clone 4G8, N-terminal, a.a. 17-24, human & mouse specific, 800708 BioLegend, CA, USA); Amyloid Precursor Protein (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Live naïve CD4+ T cells were purified from PBMCs post staining with fluorescence labelled antibodies to human CD3(OKT3, Biolegend), CD4 (RPA-T4 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Then cells were resuspended in Maxpar® cell staining buffer (Fluidigm) and blocked with Human TruStain FcX™(Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated overnight at 4 °C on a tube rotator at 18 rpm in 800 μL of PBS/0.2% BSA containing 15 nM human IgG1 (BioLegend) that had been conjugated with Alexa647 using NHS chemistry (Molecular Probes ...
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bioRxiv - Immunology 2024Quote: To measure levels of 13 soluble factors simultaneously using the LEGENDplex Human CD8/NK Panel (BioLegend, San Diego, USA), supernatants were collected from cytotoxicity assays conducted in the Maestro Z instrument at endpoint and stored at -20°C ...
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bioRxiv - Cell Biology 2024Quote: The Chemokine concentrations in cell culture media were assessed using the 13-plex LEGENDplex Human Inflammation Panel 1 (Biolegend), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Up to 20,000 cells per sample (Table S1) were stained with the TotalSeq-C Human Universal Cocktail v1 (BioLegend) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and PE/Cy7-anti-CD45 (Biolengend, 103114) or APC-anti-Epcam and PE/Cy7-anti-CD24 (BioLegend, 101821). FcX blocking solution (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used: anti-CD14APC (clone 61D3, eBioscience), anti-CD16PE (clone B73.1, eBioscience) and anti-CD16FITC (clone B73.1, Biolegend). The following antibodies were used to assess the levels of CD40 ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and detection antibodies (biotinylated mouse anti-IgG1, anti-IgG2a or anti-IgE from BioLegend) were added and incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with monoclonal antibodies anti-mouse (anti-CD45 APCcy7 [clone30-F11, Cat #103116, RRID:AB_312981], anti-CD3 BV605 [clone 172A, Biolegend, Cat #100237 ...
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bioRxiv - Immunology 2020Quote: ... according to manufacturer’s instructions kits (ELISA MAX™ Deluxe Kits from Biolegend). The detection limit was 15.6 pg/ml.
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bioRxiv - Immunology 2022Quote: ... and antibodies at a 1:200 dilution (anti-IFNγ, clone B27, Biolegend; anti-IL-17A, clone N49-653, BD Bioscience; anti-IL-22, clone 2G12A41, Biolegend; anti-IL-2, clone MQ1-17H12). Cells were washed and resuspended in FACS buffer and then analyzed on either an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... anti-CD79A (Igα; clone HM47) and anti-SYK (4D10.2) from Biolegend. The antibodies directed against ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 and anti-IFN-γ (5 μg/mL, Biolegend) for 3 days.
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bioRxiv - Genomics 2020Quote: ... PECy7-conjugated anti-CD34 (eBioscience) and FITC-conjugated anti-CD43 (BioLegend).
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bioRxiv - Immunology 2021Quote: ... anti-CD62L (FITC) and anti-CD44 (PE Cy7) surface markers (Biolegend). Cells were acquired on a FACSAria Fussion cytometer and analysis was performed using Flow Jo version 10.7.1.
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bioRxiv - Immunology 2019Quote: ... anti-CD11b (M1/70) and anti-CD45.1 (A20) were from Biolegend. Anti-ST2 (U29-93) ...
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bioRxiv - Immunology 2020Quote: ... Anti-CD69 and anti-granzyme B antibodies were purchased from Biolegend. Anti-TCF-1 antibody was purchased from Cell Signaling Technologies.
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bioRxiv - Microbiology 2020Quote: ... anti-CD103 (2µg/ml, 2E7; eBiosciences) and anti-CD69 (H1.2F3; Biolegend). Dead cells were stained with Live/Dead Fixable Near IR Staining Kit (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... FITC anti-CD16 (B73.1) and APC anti-TNF (MAb11) from BioLegend; PE anti-CD300a (E59.126 ...
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bioRxiv - Microbiology 2021Quote: ... anti-GAPDH (FF26A/F9) and anti-CRISPR Cas9 (7A9) from BioLegend; rabbit anti-HA mAb (C29F4 ...
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bioRxiv - Developmental Biology 2019Quote: ... anti-Claudin 3 and anti-MHCII (M5/114.15.2, BioLegend, 1:150) were used in the TEC analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were stained with anti-IFNγ and anti-TNFα (Biolegend) at 4°C dark for 35 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-CD45R/B220 (RA3-6B2) and anti-IL4 (11B11) from Biolegend, and anti-IFN-γ (XMG1.2 ...
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bioRxiv - Immunology 2021Quote: ... anti-CD150-PE-Cy7 or anti-BV711 (BioLegend, clone TC15-12F12.2), anti-CD117-BV605 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Purified anti-CD3ε (OKT3) and anti-CD28 (CD28.2; both from Biolegend) were used at the appropriate concentration for human T cell activation.
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bioRxiv - Immunology 2020Quote: ... anti-Ly6C (HK 1.4) and anti-D64 (X54-5/7.1, BioLegend), anti-SiglecF (E50-2440 ...
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bioRxiv - Immunology 2021Quote: ... anti-IA/IE (M5/114.15.2) and anti-BrdU (3D4) from Biolegend. Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419 ...
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bioRxiv - Immunology 2021Quote: ... Cells were activated with plate-coated anti-CD3/anti-CD28 (BioLegend, OKT3 and CD28.2 clones ...