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• Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells

  Auriane Carcone, et al., bioRxiv - Microbiology 2024

  Quote: ... PE-Cy7-coupled mouse anti-Human ICOSL (BioLegend, 309410; 1/100), Mouse anti-Gagp19 clone TP7 (Zeptometrix ...
• HIV-1 controllers possess a unique CD8+ T-cell activation phenotype and loss of control is associated with increased expression of exhaustion markers

  Amber D Jones, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were also stained for viability using the live/dead stain Zombie Yellow (BioLegend). Cell enumeration was carried out using Cytek FACSort DxP12 flow cytometer and data analysis using FlowJo v10.6.1 software.
• HIV-1 controllers possess a unique CD8+ T-cell activation phenotype and loss of control is associated with increased expression of exhaustion markers

  Amber D Jones, et al., bioRxiv - Immunology 2024

  Quote: ... and/or anti-Tim-3 (CD366) antibody (BioLegend, UltraLEAF purified anti-human ...
• The role of LEDGF in transcription is exploited by HIV-1 to position integration

  Rakesh Pathak, et al., bioRxiv - Genomics 2024

  Quote: ... RNA Pol II (8WG16 BioLegend; Cat# 664912), H3K4me3 (C42D8 Rabbit mAb #9751 cell signaling) ...
• The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication

  Abid Ullah Shah, Maged H Hemida Sr., bioRxiv - Microbiology 2024

  Quote: ... each sample was counterstained with the desired concentration (0.5ul/1 × 106 cells) of 7AAD viability staining solution (Biolegend; Cat. No. 420404) before analysis ...
• The Ex Vivo Infection of the Peripheral Bovine Mononuclear Cells (PBMCs) and the Bovine Spleen Cells with the Bovine Coronavirus (BCoV) Induced a Differential Expression of the Host Cytokine Genes Profiles and Modulates the Virus Replication

  Abid Ullah Shah, Maged H Hemida Sr., bioRxiv - Microbiology 2024

  Quote: ... the cells were resuspended with the CD3 T-cell antibody conjugated with Brilliant Violet 605 (Clone: 17A2; Cat. No. 100237) purchased from BioLegend at the desirable concentration (1:100 ...
• Polyphosphate mobilization influences the ability of Cryptococcus neoformans to cause disease in a murine model of cryptococcosis

  Kabir Bhalla, et al., bioRxiv - Microbiology 2024

  Quote: ... CD4 (RM4-5) from BioLegend; NK1.1 (PK136) ...
• Natural Killer Cell Dysfunction In Human Bladder Cancer Is Caused By Tissue-Specific Suppression of SLAMF6 Signaling

  Adam M. Farkas, et al., bioRxiv - Immunology 2024

  Quote: ... 198Pt (clone 2M2, Biolegend) antibodies ...
• Rapid metabolic regulation of a novel arginine methylation of KCa3.1 attenuates T cell exhaustion

  Piyush Sharma, et al., bioRxiv - Immunology 2024

  Quote: Antibodies from BioLegend included Pacific Blue anti-mouse Ly108 (330-AJ ...
• A mouse model of TB-associated lung fibrosis reveals persistent inflammatory macrophage populations during treatment

  Julie Boucau, et al., bioRxiv - Microbiology 2024

  Quote: ... CD11c PerCP (N418, 1:200, BioLegend), CD11b (M1/70 ...
• Corticosteroids prevent pathological angiogenesis yet compromise reparative vascular remodeling in the retina

  Masayuki Hata, et al., bioRxiv - Immunology 2024

  Quote: ... After incubation with LEAF purified anti-mouse CD16/32 (Cat# 101310; BioLegend) for 10 minutes at 4°C to block FC receptors ...
• QuickFit: A high-throughput RT-qPCR-based assay to quantify viral growth and fitness in vitro

  Nicolas M.S. Galvez, et al., bioRxiv - Microbiology 2024

  Quote: ... at 10 ng/mL (growth media) and 4 µg/mL of anti-CD28 antibody (Biolegend, #302934). Cells were plated in non-TC treated 24-well plates previously coated with 2 µg/mL of anti-CD3 antibody (Biolegend ...
• FABP4-mediated lipid accumulation and lipolysis in tumor associated macrophages promote breast cancer metastasis

  Matthew Yorek, et al., bioRxiv - Immunology 2024

  Quote: ... BV605-I-A/I-E (BioLegend), BUV737-CD11b ...
• Mitochondrial Fatty Acid Synthesis and Mecr Regulate CD4⁺ T Cell Function and Oxidative Metabolism

  KayLee K. Steiner, et al., bioRxiv - Immunology 2024

  Quote: ... TNFα PE-Cy7 (1:100 BioLegend, 506324). P-S6 PE (1:100 Cell Signaling Technologies ...
• MAIT cells have a negative impact on GBM

  Seketoulie Keretsu, et al., bioRxiv - Immunology 2024

  Quote: ... and PBS-57/CD1d-tetramers (NIH Tetramer Facility) for 20 mins and subsequently stained with the surface antibodies against CD3 (Biolegend cat #: 300324), CD4 (BD cat #:611887) ...
• Machine learning approaches identify immunologic signatures of total and intact HIV DNA during long-term antiretroviral therapy

  Lesia Semenova, et al., bioRxiv - Microbiology 2024

  Quote: ... IFN-g-PE-Cy7 (4S.B3 clone, Biolegend), IL-2-APC (MQ1-17H12 clone ...
• Machine learning approaches identify immunologic signatures of total and intact HIV DNA during long-term antiretroviral therapy

  Lesia Semenova, et al., bioRxiv - Microbiology 2024

  Quote: ... HLA-DR-APC-F750 (L243 clone, Biolegend). Antibodies used for intracellular staining were as follows ...
• The ATP-exporting channel Pannexin-1 promotes CD8⁺ T cell effector and memory responses

  Trupti Vardam-Kaur, et al., bioRxiv - Immunology 2024

  Quote: ... or phosphorylated ERK1/2 (pERK1/2; Biolegend), cells were stained with surface markers then fixed with Paraformaldehyde 1% ...
• The ATP-exporting channel Pannexin-1 promotes CD8⁺ T cell effector and memory responses

  Trupti Vardam-Kaur, et al., bioRxiv - Immunology 2024

  Quote: ... 50 μg of Treg-Protector (anti-ARTC2.2) nanobodies (BioLegend) were injected i.v ...
• Endogenous bystander killing mechanisms enhance the activity of novel FAP-specific CAR-T cells against glioblastoma

  Wenbo Yu, et al., bioRxiv - Immunology 2024

  Quote: To measure levels of 13 soluble factors simultaneously using the LEGENDplex Human CD8/NK Panel (BioLegend, San Diego, USA), supernatants were collected from cytotoxicity assays conducted in the Maestro Z instrument at endpoint and stored at -20°C ...
• Increasing Signal Intensity of Fluorescent Oligo-Labeled Antibodies to Enable Combination Multiplexing

  Madeline E. McCarthy, et al., bioRxiv - Molecular Biology 2024

  Quote: ... (2) CD8 (clone RPA-T8; Biolegend Cat: 301002) labeled with Atto488 int ...
• Increasing Signal Intensity of Fluorescent Oligo-Labeled Antibodies to Enable Combination Multiplexing

  Madeline E. McCarthy, et al., bioRxiv - Molecular Biology 2024

  Quote: ... (3) CD4 (clone RPA-T4; Biolegend Cat: 300502) labeled with Atto488/647 int ...
• Bidirectional Regulation of Motor Circuits Using Magnetogenetic Gene Therapy

  Santiago R. Unda, et al., bioRxiv - Neuroscience 2024

  Quote: ... The supernatant was transferred to a new tube with 30 µl HA.11 antibody conjugated agarose beads (Biolegend, 900801) added to each sample ...
• MAIT cells have a negative impact on GBM

  Seketoulie Keretsu, et al., bioRxiv - Immunology 2024

  Quote: ... IL-4 (Biolegend cat #: 500818), and IL-17A (Biolegend cat # ...
• Stat3-mediated Atg7 expression enhances anti-tumor immunity in melanoma

  Sarah M. Zimmerman, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and myeloid populations: anti-CD45-PerCP/Cy5.5 (BioLegend 103132), anti-CD19-FITC (BioLegend 115506) ...
• Hematopoietic stem cell division is governed by distinct RUNX1 binding partners

  Anne L. Robertson, et al., bioRxiv - Cell Biology 2024

  Quote: ... Clone: HI100 (Biolegend, 1:100), CD34-FITC ...
• Development of a humanized anti-FABP4 monoclonal antibody for treatment of breast cancer

  Jiaqing Hao, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-mouse Ly6G (Cat. #127664, Biolegend), anti- mouse NK1.1 (Cat ...
• STING-Activating Polymer-Drug Conjugates for Cancer Immunotherapy

  Taylor L. Sheehy, et al., bioRxiv - Bioengineering 2024

  Quote: ... CD223/LAG3 (C9B7W, BV785, Biolegend), CD279/PD1 (29F.1A12 ...
• Human placental organoids as a model to probe early gestation maternal immune dynamics

  Emily M. Slaby, et al., bioRxiv - Bioengineering 2024

  Quote: ... 1X brefeldin A (BioLegend catalog #420601) was added to conditions to detect Granzyme B or IFNγ if necessary ...
• SMAD4 and KRAS status shape malignant-stromal crosstalk in pancreatic cancer

  Eloise G. Lloyd, et al., bioRxiv - Cancer Biology 2024

  Quote: ... cells were stained for 30 min on ice with anti-mouse CD31-PE/Cy7 (102418; BioLegend; RRID:AB_830757), CD45-PerCP/Cy5.5 (103132 ...
• SMAD4 and KRAS status shape malignant-stromal crosstalk in pancreatic cancer

  Eloise G. Lloyd, et al., bioRxiv - Cancer Biology 2024

  Quote: ... CD45-PerCP/Cy5.5 (103132; BioLegend; RRID:AB_893344), CD326 (EpCAM)-AlexaFluor 488 (118210 ...
• GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

  Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... APC conjugated anti-human ganglioside GD2 antibody (#357306; Biolegend) or APC-Mouse-IgG2a,κ isotype control (#400220 ...
• GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

  Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... or APC-Mouse-IgG2a,κ isotype control (#400220; Biolegend) was used for IHC.
• GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

  Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and live cells stained with APC anti-human GD2 (#357306; Biolegend) (isotype control, APC Mouse IgG2a, k; #400220, Biolegend) or Anti-human/mouse CD49f antibody (#313620 ...
• GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

  Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and live cells stained with APC anti-human GD2 (#357306; Biolegend) (isotype control ...
• GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

  Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... #917701 from Biolegend) with isotype control (purified mouse IgG3 ...
• GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

  Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... or Anti-human/mouse CD49f antibody (#313620, Biolegend) (isotype control ...
• Patient-derived xenografts and single-cell sequencing identifies three subtypes of tumor-reactive lymphocytes in uveal melanoma metastases

  Joakim Karlsson, et al., bioRxiv - Cancer Biology 2024

  Quote: ... CD137 (PE and 4B4-1, BioLegend), active caspase-3 (clone C92-605 ...
• Patient-derived xenografts and single-cell sequencing identifies three subtypes of tumor-reactive lymphocytes in uveal melanoma metastases

  Joakim Karlsson, et al., bioRxiv - Cancer Biology 2024

  Quote: ... CD8 (APC and SK1, BioLegend), CD107a (APC and H4A3 ...
• Patient-derived xenografts and single-cell sequencing identifies three subtypes of tumor-reactive lymphocytes in uveal melanoma metastases

  Joakim Karlsson, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and the co-culture was analyzed using human CD3 (FITC and HIT3a, BioLegend), CD8 (APC and SK1 ...
• Inhibition of O-GlcNAc transferase activates type I interferon-dependent antitumor immunity by bridging cGAS-STING pathway

  Jianwen Chen, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and stained with anti-IFN-γ (XMG1.2) and anti-TNF-α (MP6-XT22) from BioLegend. For intranuclear Foxp3 staining ...
• Inhibition of O-GlcNAc transferase activates type I interferon-dependent antitumor immunity by bridging cGAS-STING pathway

  Jianwen Chen, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-CD25-PE-Cy5 (PC61) from BioLegend. For intracellular cytokine staining of tumor-infiltrating lymphocytes (TILs) ...
• Antibody-lectin chimeras for glyco-immune checkpoint blockade

  Jessica C. Stark, et al., bioRxiv - Bioengineering 2024

  Quote: ... CD16 blocking antibody (clone 3G8, BioLegend) was added at 10 μg/mL ...
• Asparagine availability controls B cell homeostasis

  Yavuz F. Yazicioglu, et al., bioRxiv - Immunology 2024

  Quote: ... Apoptosis was quantified by annexin V-FITC binding (Biolegend). The proliferation index was determined using the Proliferation module of FlowJo (BD) ...
• The polarity protein Yurt associates with the plasma membrane via basic and hydrophobic motifs embedded in its FERM domain

  Clémence L. Gamblin, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-HA (BioLegend); anti-Actin (Novus Biologicals) ...
• The dynamics and longevity of circulating CD4⁺ memory T cells depend on cell age and not the chronological age of the host

  M. Elise Bullock, et al., bioRxiv - Immunology 2024

  Quote: ... CD44 BV785 (all BioLegend), CD62L BUV737 (BD Biosciences) ...
• Computational identification of surface markers for isolating distinct subpopulations from heterogeneous cancer cell populations

  Andrea L. Gardner, Tyler A. Jost, Amy Brock, bioRxiv - Cancer Biology 2024

  Quote: ... Cells were resuspended in cell staining buffer (PBS + 5 mM EDTA + 1% BSA + 1.6 mM NaOH + 0.01% sodium azide) and incubated in 1:100 diluted Zombie UV viability dye (Biolegend, 423107) or Zombie Violet viability dye (Biolegend ...
• TNBC response to paclitaxel phenocopies interferon response which reveals cell cycle-associated resistance mechanisms

  Nicholas L Calistri, et al., bioRxiv - Cancer Biology 2024

  Quote: Experiment 2: All conditions were multiplexed using Hashtag Oligonucleotide barcoding technology (TotalSeq-B, Biolegend) following manufacturer standard protocol ...
• Heterotypic competition between cancer cells and hepatocytes generates heterogeneous context-dependent phenotypes

  Andrea Spinazzola, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 20 µg/mL in Annexin V Binding Buffer (Biolegend #422201). Cells were then run into a flow cytometer and the results were analyzed using FlowJo ...
• Heterotypic competition between cancer cells and hepatocytes generates heterogeneous context-dependent phenotypes

  Andrea Spinazzola, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and stained for 15 minutes with Annexin V Pacific Blue (Biolegend #640918) 2 µL/100 µL and propidium iodide (Sigma #P4170 ...