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Citations for BioLegend :
2801 - 2850 of 4284 citations for 2 Methoxy 2 3 4 5 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with ice cold PBS and surface stain with primary anti-PD-L1 (1:50) antibody (BioLegend) on ice for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... immunohistochemistry (IHC) and flow-cytometry (FC): Pacific Blue anti-mouse Ly-6A/E (Biolegend, Cat#108120, 1:50 FC), Sca-1/Ly6A/E (E13 161-7 ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions were first washed in PBS and then incubated with 50 μl Zombie UV viability dye (BioLegend) for 10 min at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2021Quote: ... The first surface marker panel included: BV421 and PerCP/Cy5.5 anti-TIM-3 (clones B8.2C12 and RMT3-23, BioLegend), BV510 anti-PD-1 (EH12.1) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 washes were performed prior to secondary antibody staining with anti-rabbit bv421 1:500 (BioLegend 406410) with 3 additional washes after secondary antibody ...
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bioRxiv - Synthetic Biology 2024Quote: ... and stained for the intracellular accumulation of IFNγ and TNFα (Supp. Table 3) in 1x Permeabilization Buffer (Biolegend). Samples were analyzed using a Fortessa LSR flow cytometer (BD ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST ...
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bioRxiv - Immunology 2021Quote: ... 211-11A; Pepro Tech), IL-1β (1 ng/ml, 211-11B; Pepro Tech) or IFNα (100 ng/ml, 752802; Biolegend). Cytokines used in Figure 1 - figure supplement 1D were added at the concertation shown in Supplementary Table 1.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded at a density of 2.5 × 104/well in 96-well plates pre-coated with 1ug/ml CD3 (Biolegend100339) and 1ug/ml CD28 (Biolegend 102115) antibodies and cultured in 1640 media with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Effector function was assessed by stimulating cells for 4 hours with PMA (20 ng/ml) and Ionomycin (1 μg/ml) with GolgiStopTM and monitored for CD8-PerCP (RPA-T8, Biolegend), CD4-AF700 (RPA-T4 ...
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bioRxiv - Bioengineering 2022Quote: ... Cytokine-primed BMDMs were cultured in RPMI growth media + 20 ng/mL M-CSF + 20 ng/mL IFNγ (BioLegend 575302) or 20 ng/mL IL-4 (BioLegend 574302 ...
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bioRxiv - Immunology 2020Quote: ... human Interlukin-6 (IL-6) (100 ng/ml) and human IL-6Rα (100 ng/ml) (BioLegend, San Diego, CA, USA).
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Bioengineering 2022Quote: ... were modified by mixing a solution of nanovials in washing buffer with a cocktail of 40 µg/mL streptavidin and 20 µg/mL TotalSeqC conjugated streptavidin (Biolegend) solution at equal volumes ...
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bioRxiv - Cancer Biology 2022Quote: ... and activated using plate-bound anti-CD3 (1μg/ml; clone-145-2C11) and anti-CD28 (5μg/ml; clone-37.51) antibodies (Biolegend; San Diego, CA) for 4 days.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were labeled with the following antibodies:(CEACAM6-FITC at 10 μg/mL, Sino Biological 10823-R408R; H2Kb-Pac Blue at 0.5 mg/mL, BioLegend 116514 ...
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bioRxiv - Immunology 2023Quote: ... along with co-stimulatory antibodies (CD28 and CD49d at 1 μL/mL) and Brefeldin-A (1 μL/mL, BioLegend, USA). LIVE/DEAD and surface staining was performed as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with anti-CD3/CD28 (3.5 µg/ml anti-CD3 mAb, BioLegend, #100339; 1 µg/ml anti-CD28 mAb, BioLegend, #102115) in the presence of 5 μg/mL Brefeldin A (BFA ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 5×106 cells/mL in RPMI media with 10% FBS and 30ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF; BioLegend). After 48 hours and again after 120 hours from initial plating cell culture media was replaced with fresh media containing M-CSF ...
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bioRxiv - Immunology 2024Quote: ... CTLs were seeded on sapphire discs coated with poly-L-ornithine (0.1 mg/ml) and anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402) in flat specimen carriers (Leica) ...
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bioRxiv - Immunology 2024Quote: ... which were coated with poly-L-ornithine (0.1 mg/ml) and mouse anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402), for 10 min at RT (20°C ± 2°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 200 ng mL-1 anti-human RP105 (Biolegend), 500 U mL-1 IL-2 (UCSF Pharmacy) ...
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bioRxiv - Biophysics 2021Quote: ... E-cadherin neutralizing antibody (Biolegend, 10 μg/ml), NSC23766 (Abmole ...
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bioRxiv - Genetics 2021Quote: ... and 20 ng/ml mouse M-CSF (BioLegend) for the purpose of differentiation ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5 μg/mL anti-CD28 (Clone 37.51, Biolegend), and 100 U/mL rmIL-2 (402-ML-100 ...
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bioRxiv - Immunology 2021Quote: ... 10 μg/ml HLA-DR (clone L243, BioLegend), HLA-DQ (clone SPVL3 ...
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bioRxiv - Immunology 2021Quote: ... IFN-γ (20 ng/ml, Biolegend, Cat#570206) for 3 days and stained with anti-human CD19 ...
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bioRxiv - Immunology 2021Quote: ... IL-21 (10 ng/ml, Biolegend, Cat#571204), IFN-γ (20 ng/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... and cultured with 2μg/ml soluble αCD28 (Biolegend). The following conditions were specific to each differentiation regime ...
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bioRxiv - Cancer Biology 2020Quote: ... 10μg/ml anti-CD11b or anti-TLR2 (Biolegend) or 1μg/ml fibronectin inhibitor (Santa-Cruz Biotechnology ...
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bioRxiv - Genomics 2020Quote: ... and 1 μg/mL anti-CD28 (BioLegend 302944) soluble antibodies for 18 h ...
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bioRxiv - Microbiology 2021Quote: ... and 20ng/mL M-CSF (BioLegend, Cat: 574802) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... and IL-10 (2µg/ml, JES5-16E3; Biolegend) was performed on ice for 30 minutes.
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bioRxiv - Microbiology 2020Quote: ... anti-MHC-II (2µg/ml, M5/114.15.2; Biolegend), anti-CD103 (2µg/ml ...
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bioRxiv - Immunology 2022Quote: ... and TCRβ-Pacific Blue (0.3 μg/mL; BioLegend). All samples were analyzed on a Becton-Dickinson LSR-II/Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 30ng/ml CD28 (clone 37.51, 102112, BioLegend) antibodies ...
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bioRxiv - Immunology 2022Quote: ... or with 10 ng/ml rmIL-1ý (Biolegend) + 20 ng/ml rmIL-23 (R&D) ...
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bioRxiv - Immunology 2020Quote: ... and 1 μg/ml of human CD3ε (BioLegend). When needed ...
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bioRxiv - Microbiology 2020Quote: ... 0.01 mg/ml donkey anti-rabbit AF555 (Biolegend), and a 1:2000 dilution of phalloidin-iFluor 647 reagent (Abcam) ...
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bioRxiv - Cell Biology 2021Quote: ... 40 ng/ml mTPO (Biolegend, Cat. No. 593302) and 10 ng/ml IL3 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... or streptavidin-PerCP-Cy5.5 (BioLegend, 0.4 µg/ml). Cells were stained for 30 min in the dark at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CD28 (1 µg/ml) (Biolegend, San Diego, CA), and IL-2 (20 ng/ml ...
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bioRxiv - Biophysics 2019Quote: ... 10 ng/ml basic Fibroblast Growth Factor (Biolegend). All reagents were from ThermoFisher Scientific if another supplier is not stated.