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Citations for BioLegend :
2751 - 2800 of 3014 citations for Recombinant Human ENG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected on day 6 and immediately assessed for cytokine and chemokine secretion by LEGENDplex™ using LEGENDplex Human Inflammation Panel 1 (13-plex) with V-bottom Plate (Biolegend catalog #740809 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-HA and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The secretion levels of IL-1β and TGF-β were quantified using enzyme-linked immunosorbent assay (ELISA) by human IL-1β ELISA Max Deluxe kit (437004, BioLegend) and human TGF-β1 duoset ELISA kit (Dy204-05 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... washed cells were resuspended in 100 μL of FACS buffer with 5 μL of PE anti-human TMPRSS2 antibody (BioLegend #378403) per million cells and incubated for 30 min at 4 °C in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
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bioRxiv - Immunology 2024Quote: ... cell pellets resuspended in 100 μl FACS buffer (phosphate buffer saline containing 1% BSA and 0.1% sodium azide) were treated with human TruStain FcX™ (Biolegend #422302) for 15 min on ice followed by incubation with rabbit anti-human LBR antibody (Abcam #32535 ...
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bioRxiv - Immunology 2019Quote: ... then incubated with antibodies specific for intracellular proteins and Brilliant Violet 605-streptavidin conjugate (BioLegend) to detect biotin-conjugated antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were analyzed by SDS-PAGE and immunoblotting with anti-GST (BioLegend MMS-112P) and anti-His (Qiagen 34660 ...
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bioRxiv - Immunology 2020Quote: Interferon beta (IFNβ) protein levels in plasma were measured using Legendplex Mouse Inflammation Panel (Biolegend).
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bioRxiv - Immunology 2021Quote: ... Bio-PyCSP protein (38.3 kDa) at 16 μM was combined with SA-APC antibody (Biolegend) at 2 μM ...
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bioRxiv - Immunology 2021Quote: The following antibodies have been used to stain extracellular proteins: anti-CD8-Alexa488 (Biolegend 100723), anti-PD1-PE (eBioscience 12-9981-83) ...
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bioRxiv - Microbiology 2019Quote: ... The following antibodies were used for detection of proteins of interest: HA.11 (mouse, Biolegend), β-actin (mouse ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were analyzed by SDS-PAGE and immunoblotting with anti-GST (BioLegend MMS-112P) and anti-His (Qiagen 34660 ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: Synchronized EL4 cells were stimulated by Ig Protein with anti-CD3 (final 2μg/ml, Biolegend) conjugated magnetic beads ...
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bioRxiv - Immunology 2022Quote: ... endogenous biotin and avidin proteins were successively blocked using an Avidin/Biotin Blocking Kit (Biolegend), for 10 min each ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were utilized for immunoblotting: myelin basic protein (MBP; Biolegend; 808401; 1:1000), PLP (rat hybridoma ...
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bioRxiv - Immunology 2024Quote: ... and FcεRIγ (FCABS400F antibody, Milli-pore)10 protein expression on live (Zombie red, 77475; BioLegend), CD3-negative (300318 antibody ...
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bioRxiv - Immunology 2024Quote: The following antibodies have been used to stain extracellular proteins: BV711 anti-CD45RA (Biolegend 304138), APC anti-CD45RO (Biolegend 304210) ...
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bioRxiv - Cell Biology 2024Quote: Flow cytometry and ImageStream reagents: Antibodies against the following proteins were used: CD41 (MWReg30, Biolegend), Ter119 (Ter119 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration in the supernatant was measured by Bradford assay (BioLegend, San Diego, CA, USA). Proteins (10-40 μg ...
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bioRxiv - Cell Biology 2020Quote: ... The following FACS antibodies were used at 1:200: mouse anti-human CD45 APC-Cy7 (BioLegend; Cat#304014; used at 1:200), mouse anti-human anti-CD11b-APC-Cy7 (BD ...
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bioRxiv - Immunology 2021Quote: ... the supernatants were collected to determine the levels of antibody productions (e.g., IgG1, IgG2, IgG3, IgG4, IgA, and IgM) by using LEGENDplex™ Human Immunoglobulin Isotyping Panel (Biolegend, CA, USA). The Gallios Flow Cytometer was utilized to analyze the data according to the manufacturer’s recommended protocol.
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bioRxiv - Immunology 2021Quote: Fluorophore- or biotin-conjugated antibodies specific for human cell-surface antigens and cytokines were as follows: anti-CD71 (CY1G4, BioLegend, DF1513, NovusBio), anti-CD235a (HI264 ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Genomics 2020Quote: ... The cells were centrifuged at 300xg for 5 minutes at room temperature and resuspended in 85 ul of staining buffer and incubated with 5 ul of Human TruStain FcX(Biolegend cat: 422301) for 10 minutes on ice in 5 ml FACS tubes ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: For bead-based isolation of human CCR5+ and CCR5− cPMN were sorted using anti-APC MojoSort™ nanobeads (Biolegend, San Diego, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2020Quote: ... up to 1×106 EnSC/100 µL of Magnetic Bead Buffer (0.5% BSA in PBS) were combined with 5 µL/1×106 cells phycoerythrin (PE) conjugated anti-human SUSD2 antibody (BioLegend, London, UK). After 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... of the sorted MBS-MEF and MCF7 cell populations was then counterstained with Alexa-488-labeled anti-human CD326 (Biolegend; Cat #: 324210) and PE-labeled anti-mouse CD321 antibody (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell surface marker expression in the SORE6+ and SORE6-compartments of MDA-MB-231 cells cultured in vitro or recovered from primary tumors was assessed by flow cytometry following staining with Brilliant Violet 421TM anti-human CD133 (Biolegend Cat#372807), or APC anti-human CD44 (Biolegend Cat#338805 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... with 1 × 106 cells/mL 5000 rad irradiated PBMC + 50 U/mL IL-2 + 10 ng/mL IL-15 (both from NCI BRB Preclinical Biologics Repository) + 0.1 μg/mL each of anti-CD3 (Ultra-LEAF purified anti-human CD3 antibody clone OKT3; BioLegend, San Diego, CA) and anti-CD28 (Ultra-LEAF purified Anti-human CD28 antibody clone 28.2 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were then evaluated for IFN-γ levels using the Legend Max ELISA kit with pre-coated plates (human IFN-γ) (Biolegend). Surface phenotype of cells that had been removed from the well was determined by flow cytometry using fluorochrome conjugated antibodies specific for 4-1BB or CD69.
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Immunology 2019Quote: ... according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 μg/mL anti-human RP105 antibody clone MHR73-11 (BioLegend Cat.# 312907).
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... P815 cells were used as target cells for human CTLs and pulsed for 1h at 37°C with 1µg/ml anti-CD3 antibody (clone UCHT1, Biolegend, Cat no. 300438). They were washed and resuspended at 1x105 cells/ml in killing assay media (RPMI without phenol red + 2% FCS ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Human pluripotent stem cell-derived cardiomyocytes used for IF-FISH were sorted by gating for SIRPA+ (PE-Cy7 anti-human CD172a/b, Biolegend, 1:200) and CD90- (APC anti-human CD90 (Thy1 ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... HLA-A2 positive PBMC samples were further stained with PE labelled tetramer (home-made) and APC labelled human CD8+ antibody (BioLegend Cat#344721), and acquired with flow cytometer FACS Canto (BD).