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Citations for BioLegend :
2751 - 2800 of 10000+ citations for Rabbit Anti Human IgG Fc Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... after saturation of non-specific sites with Humain TruStain FCX FC Blocking Reagent (Biolegend). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Th1 cell released cytokines were analyzed by LEGENDplexTM Human Th1 Panel (Biolegend, Cat# 741035). Five human T cell cytokines including IL-2 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with different primary antibodies (Rat-anti-MBP, Sigma-Aldrich MAB386, 1:1000, monoclonal; Rabbit-anti-GFAP, CiteAb Z0334, 1:1000, polyclonal; Mouse-anti-6E10 Amyloid-β, BioLegend SIG-39300, 1:1000, monoclonal) diluted in 200 μl 10% NDS and 0.4% Triton-X blocking medium ON at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunostained with the following antibodies: rabbit anti-IFITM3 (Cat. nr. AP1153a, Abcepta, 1:500) and mouse anti-HA (clone 16B12, BioLegend, 1:1400). Secondary antibodies conjugated to Alexa® Fluor 488 or 647 (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then incubated overnight at 4°C with primary antibody diluted in blocking solution (rabbit anti-FLAG, Sigma, 1:250 or mouse anti-Tuj1, Biolegend 1:500). After washing in PBS and incubation with secondary antibody (Alexa 647 anti-goat IgG 1:500 ...
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bioRxiv - Systems Biology 2024Quote: ... following staining with anti-Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor® 488 Conjugate) (CST, #41622) and anti-HA Alexa647 (Biolegend, #682404) at 1:200 dilution.
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bioRxiv - Biochemistry 2020Quote: ... and IgG-APC-CD147 (BioLegend, Cat. 306214) in a final volume of 50 µL adding to 100 µL of EV-Clean for 30 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... EpCAM-APC-Cy7 Mouse IgG (Biolegend 324245), and Integrin-alpha-PE Rat IgG (Biolegend 313607 ...
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bioRxiv - Developmental Biology 2024Quote: ... CD38 PerCP-Cy5.5 Mouse IgG (Biolegend 303522), EpCAM-APC-Cy7 Mouse IgG (Biolegend 324245) ...
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bioRxiv - Molecular Biology 2024Quote: ... or IgG (Biolegend, Cat# 400959, Clone HTK888) at day 0 ...
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bioRxiv - Neuroscience 2022Quote: ... goat IgG Smi311 & Smi312 (1:1000, BioLegend), rabbit IgG anti-App (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and stained in 100 μl for 30 minutes on ice using an anti-mouse IgG-Brilliant Violet421 secondary antibody at 1:90 (BioLegend, 405317). Cells were again washed twice ...
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bioRxiv - Cell Biology 2021Quote: ... and the secondary Alexa Fluor donkey 488-conjugated anti-mouse IgG pAb (Thermo Fischer Scientific, USA) and the Alexa Fluor 667-conjugated rat anti-mouse CD31 mAb (clone MEC133, Biolegend, USA).
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bioRxiv - Microbiology 2021Quote: ... Plates were washed again and then incubated with horse radish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Biolegend; 1:3000) for 1 hour at 37°C ...
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bioRxiv - Immunology 2020Quote: ... was incubated with 1-2 spike+ cells for 1 hour at 4°C before adding of 0.02 ug/ml ACE2-mFc followed by FITC conjugated anti-mouse IgG (Biolegend, cat#405305) and the MFI of FITC and positive percentage were collected.
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bioRxiv - Developmental Biology 2023Quote: ... Then cells were again washed and followed by an incubation with anti-mouse IgG coupled to PE-Cy7 (Biolegend ref 406613) at a 1/200 dilution in BD wash buffer during 20 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary and secondary antibodies were used: MHCII-Biotin (BioLegend, Catalog No. 107603, 1/200), UEA1 (Vector Laboratories Catalog No ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12p70 and recombinant human IL-18 at 10ng/ml (Biolegend; San Diego, CA).
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Immunology 2021Quote: Cells were stained with the following surface antibodies: Anti-human CD8a FITC (1:100, clone RPA-T8, BioLegend, cat # 301050), Anti-human TCRgd FITC (1:100 ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used for coating of culture plates for in vitro T cell activation: anti-human CD3 (clone HIT3α; Biolegend), anti-human CD28 (clone CD28.2 ...
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bioRxiv - Bioengineering 2019Quote: ... MCF-7 cells in EPB were stained with a 1:20 dilution of FITC Anti-human CD326 (Biolegend, Cat.no. 324204) for 30 min at 4 °C to allow for population tracking in flow cytometry ...
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Genomics 2019Quote: ... Measurement of BCL-6 expression in targeted Jurkat lines was assessed by flow cytometry using anti-human APC-BCL-6 (Biolegend) after treatment with human recombinant IFNg (5 ng/mL ...
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bioRxiv - Genomics 2019Quote: ... Expression of Ikaros and CXCR5 by targeted Jurkat lines was also assessed by flow cytometry using anti-human APC-CXCR5 (Biolegend) and anti-human PE-Ikaros (BD Biosciences) ...
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bioRxiv - Genomics 2021Quote: ... and then up to 500,000 cells from each condition were stained with a unique TotalSeq™-A anti-human Hashtag antibody (BioLegend) in 50 ul for 30 minutes at 4°C on a Laminar Wash™ 16-well strip (Curiox Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin) with mouse anti-human monoclonal antibody CD62P-AlexaFluor647 (Clone AK4, Cat.No. 304918, BioLegend, USA). and activation associated conformational change in integrin αIIbβ3 was detected with a mouse anti-human monoclonal antibody PAC1-FITC (Clone PAC-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... while the MDR1 replicons were stained with 5 μL of PE anti-human CD243 (ABCB1) antibody clone 4E3.16 (Biolegend, US). After staining for 30 min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... PE anti-human TRAILR4 (DcR2) (R&DSystems, Clone 104918) or PE Mouse IgG1 κ Isotype Control (Biolegend, Clone MOPC-21) for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Physiology 2022Quote: ... the cell pellet was resuspended in 100 μl of Alexa Fluor 647-conjugated anti-human/mouse SSEA1 antibody (catalog no. MC-480; BioLegend) diluted 1:100 in PBS + 2.5% FBS and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were sorted by fluorescent assisted cell sorting on the SH800S cell sorter (Sony Biotechnology) using the following antibodies: FITC anti-human lineage cocktail (Cat# 343604, BioLegend) and APC anti-human CD34 (Cat# 343509 ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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bioRxiv - Developmental Biology 2022Quote: ... hPGCLCs were dissociated into single cells and then stained with Alexa Fluor 647-conjugated rat anti-human PDPN (337007, BioLegend) and BV421-conjugated anti-CD49f (INTEGRINα6 ...
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bioRxiv - Immunology 2022Quote: ... Subsequent iterative cycles of standard immunohistochemistry were performed using the following anti-human primary antibodies: podoplanin (D240, BioLegend; 1:100), LYVE-1 (rabbit polyclonal ...
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bioRxiv - Immunology 2019Quote: ... the cells were thawed and transferred to a 96-well plate and stained for viability with Viability Dye eFluor 506 (eBioscience) followed by incubation with the surface antibodies: anti-human CD3 AF700 (clone UCHT1, Biolegend), CD4 BV785 (clone OKT4 ...
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bioRxiv - Immunology 2019Quote: ... cells were sorted from PBMC using a BD FACSAria cell sorter after staining with anti-human CD4 PE-conjugated antibody (Cat. #317410, Biolegend) or separated by human CD4+ T cell isolation kit (Cat ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1-2 million HL-60 and THP-1 cells were resuspended in PBS containing 1% fetal bovine serum and stained with PE anti-human CD11b antibody (Biolegend). Stained cells were analyzed on FACScalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2019Quote: ... Expression of the chimeric receptor was determined by staining with AlexaFlour 647-conjugated anti-3S193 idiotype [supplied by Ludwig Institute for Cancer Research (LICR)] and truncated CD34 was detected with PE-conjugated anti-human CD34 (BioLegend). Cell surface phenotyping of transduced cells was determined by staining with BV785-conjugated anti-human CD3 (UCHT1 ...
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bioRxiv - Immunology 2019Quote: ... GolgiPlug™ and GolgiStop™ were added 3 h before cells were processed for surface and intracellular staining for CD4 (BV785-conjugated anti-human; OKT-4, BioLegend), CD8 (APC-H7-conjugated anti-human ...
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bioRxiv - Immunology 2020Quote: ... for 8 hours and then surface staining was performed using the following abs anti-human BDCA-2-PE (clone AC144; Biolegend), CD123-PeCy7 (clone 6H6 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed with FACS buffer and labeled with PE conjugated mouse anti-human TNFRSF12A antibody (clone ITEM-4, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
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bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...