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Citations for BioLegend :
2751 - 2800 of 2829 citations for CD32 Human R167 HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Supernatants from triplicate wells of each condition were collected at 24 hrs and assayed for the IL-8 concentration using a human IL-8 enzyme-linked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (BioLegend, San Diego, CA). IL-8 values were reported as normalized values defined as a proportion increased or decreased compared to values obtained for J99 ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Aβ peptides were extracted from 250 µL human plasma using immunoprecipitation with anti-β-Amyloid 17-24 (4G8) and anti-β-Amyloid 1-16 antibodies (6E10, both Biolegend®) coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads (Thermofisher ...
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to the staining of 332 surface antigens using LEGENDScreen Human Cell Screening PE Kit (cat.no. 700001; Biolegend, San Diego, CA, USA). Data acquisition was performed on FACSVerse (Becton Dickinson (BD) ...
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bioRxiv - Cancer Biology 2020Quote: ... We split our single cell suspension in half and stained half of the uncultured HCI010 and HCI002 cells for FACS using fluorescent labeled antibodies for human specific CD298 (Biolegend, cat. no. 341704) and mouse-specific MHC-I (eBioscience ...
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bioRxiv - Neuroscience 2022Quote: ... ELISA kit (CSB-E18033h, Generon) and monomeric α-Syn was analyzed using LEGEND MAX™ Human α-Synuclein ELISA Kit (SIG-38974, BioLegend). Then it was normalized by total protein per well using Pierce BCA Protein Assay Kit (23225 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 20 minutes on ice with 1µg of BioLegend TotalSeq-A anti-human hashtag oligo antibody (BioLegend TotalSeq-A0251 to A0258). Cells were washed 3 times then pooled at equal ratios for sequencing ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130, San Diego, CA) following manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Biochemistry 2022Quote: Moles of product formed in hydrolysis reactions used for ACE2 activity and specificity profiling were determined by comparing area under the curve for LC chromatograms corresponding to these reactions to areas under the curve observed for control reactions in which > 95% input substrate conversion was achieved by incubation of substrate with 25 nM recombinant human ACE2 commercial standard (BioLegend, San Diego, CA) for between one and four hours pending peptide substrate ...
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bioRxiv - Pathology 2019Quote: ... IL-9 and IL-6 determinations (Human IL-4, IL-5, IL-9 and IL-6 MAX, Biolegend, San Diego, CA 92121 USA) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... IHC staining of mast cell tryptase was carried using mouse anti-human Mast Cell Tryptase Antibody (catalog # 369402, RRID:AB_2566541, BioLegend®, San Diego, CA, USA) [performed by the Northwestern University Mouse Histology and Phenotyping Core] ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested PDX or EHS tumors were processed to single cells47 and stained with PE-conjugated anti-human CD298 PE (BioLegend; 1:50 dilution) and FITC-conjugated anti-mouse CD45 FITC (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... was used to detect T cells and the following anti-human antibodies were used to identify T cell subsets: anti-CD137-PeCY7 (BioLegend Cat# 309818, RRID:AB_2207741), anti-CD103-BV605 (BioLegend Cat# 350218 ...
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bioRxiv - Pathology 2019Quote: ... The ears were fixed in 4% paraformaldehyde for 20 minutes at 4°C and incubated overnight in 0.05% Triton X-100 in PBS containing anti-human CD31 (Biolegend, San Diego, CA, USA) antibody conjugated to either PE or AF647 in a 1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with 2 ml of FACS buffer followed by 300 μl of FACS buffer and resuspended in a total of 100 μl of Brilliant Violet 650 mouse anti-human CD45 (Biolegend catalog no. 304043), Alexa Fluor 700 mouse anti-human CD4 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then resuspended in 0.2µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG, Jackson Immunoresearch 1:200, Brilliant Violet 421-anti-Rabbit IgG, Biolegend 1:200). This was incubated for 10-60 min before cells were washed as above and resuspended for acquisition on a BD LSRII or Beckman Coulter Cytoflex S ...
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bioRxiv - Immunology 2021Quote: ... Media supernatants were used to measure released cytokines by BAL cells using a 13-plex bead-based immunoassay and a human macrophage LEGENDplex (Biolegend, San Diego, CA).
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bioRxiv - Cell Biology 2022Quote: Cytokine levels in the plasma and cell culture medium were detected using the LEGENDplex™ Human Th Panel (BioLegend, San Diego, CA, USA) containing 13 human antibodies targeting IL-5 ...
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bioRxiv - Immunology 2022Quote: Primary human neutrophils isolated as described were stained at room temperature for 20 minutes with 5 μM Calcein AM (BioLegend, Cat. No. 425201) and applied to the top well of a fluorescence-blocking 24-well transwell plate with 3 μm pores (Corning ...
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bioRxiv - Immunology 2022Quote: Activated γδT cells were seeded in round-bottom plates with FITC conjugated anti-mouse CD107a (1D4B) antibody or anti-human CD107a (H4A3) (Biolegend, San Diego, CA). γδT cells were co-cultured with A20 or K562 at a ratio of 1:1 ...
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bioRxiv - Neuroscience 2022Quote: ... human Aβ was immunodepleted from TgCM with the mouse antibody 6E10 (Purified anti-β-Amyloid, 1-16 antibody BioLegend Cat# 803004, RRID: AB_2715854) and Protein G Sepharose beads (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... blood cells were lysed using VersaLyse buffer and blood and CSF cells were stained using the following anti-human antibodies (Biolegend; clone names indicated): CD3 (UCHT1) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Bioengineering 2024Quote: The supernatant of CAR T and cancer co-culture systems was measured for their expression of cytokines using the LegendPlex Human Essential Immune Response Panel (Cat # 740930, BioLegend, San Diego, CA) (IL-4 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed twice with 2% FACS buffer (1X PBS and 2% BSA) centrifuged and stained with human TruStain FcX (Biolegend, San Diego, CA) for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: Apical supernatant from cells at each harvest time point was assessed using LEGENDPlex Human Anti-Virus Response Panel (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions and using a FACSCanto II flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Samples were stained immediately after fixation with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with PBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend, Catalog #101207, 1:50) was added to measure and gate on differentiated HL-60 cells ...
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bioRxiv - Bioengineering 2023Quote: ... coated glass bottom chamber and then labeled with 1ugꞏml−1 of Alexa Flour (AF) 488 conjugated anti-human CD45 antibodies (Biolegend, Cat# 304017, clone HI30) in 2% BSA in PBS for 20 min at RT ...
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bioRxiv - Immunology 2023Quote: ... prior to the end of the CD45- sort one vial of TotalSeq-C Human Universal Antibody Cocktail v1.0 (Biolegend, San Diego, CA, USA) was equilibrated to room temperature for 5min and then spun at 10,000g for 30 seconds ...
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bioRxiv - Cancer Biology 2023Quote: ... dendritic cell markers were purchased for characterization of immune cells using flow cytometry: PerCP/Cy5.5 anti-human CD3 and CD11c (Biolegend, San Diego, CA, USA), PE anti-human CD4 and CD56 (Biolegend) ...
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bioRxiv - Biophysics 2023Quote: ... Flow cytometry is further used to determine the expression profile of the integrins CD29 (PE anti-human CD29 antibody; Biolegend, cat. no. 303003) and CD18 (PE anti-human CD18 antibody ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the NK-92 staining panel: anti-CD107a (LAMP-1) FITC (BioLegend catalog #328606, clone H4A3), anti-CD56 (NCAM ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for T cells: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD26 PerCP/Cy5.5 (BioLegend catalog #356112 ...
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bioRxiv - Immunology 2024Quote: ... and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3, BioLegend, cat. 317325) and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2 ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Cancer Biology 2022Quote: ... was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (Biolegend, San Diego, CA, USA). Mice were monitored closely to detect disease onset and treatment started when hCD45+ cells were detectable in the peripheral blood ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were used for these studies: anti-human CD47-APC (1µl/106 cells in 100µl; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3), anti-human CD47-FITC (5µl/106 cells in 100µl ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were blocked with 0.5 % BSA for 10 min on ice and incubated with the primary mAbs biotin-anti-human CD274 (B7-H1, PD-L1) (clone 29E.2A3, BioLegend, San Diego, CA, USA) or biotin anti-mouse CD274 (clone 10F.9G2 ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...