Labshake search
Citations for BioLegend :
2651 - 2700 of 3250 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... Then cells were resuspended in Maxpar® cell staining buffer (Fluidigm) and blocked with Human TruStain FcX™(Biolegend) for 10 minutes at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
-
bioRxiv - Cell Biology 2023Quote: ... Osaka University) were pre-incubated with anti-human-Fc secondary antibodies fused to APC (BioLegend, #410712, dilution 1:100) for 1 h in 1% BSA in PBS ...
-
bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
-
bioRxiv - Immunology 2023Quote: ... aureus Newman (from 2.106 to 2.104/well) in the presence of anti-human CD107a-AF647 mAb (clone H4B4, Biolegend, France) added at the beginning of stimulation in serum and antibiotic free RPMI media in 96 well round bottom plates ...
-
bioRxiv - Immunology 2023Quote: ... Each B cell sample was split into two aliquots of at least 500,000 cells for labeling with different TotalSeqC anti-human cell hashing antibodies (BioLegend). Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences ...
-
bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and incubated overnight at 4 °C on a tube rotator at 18 rpm in 800 μL of PBS/0.2% BSA containing 15 nM human IgG1 (BioLegend) that had been conjugated with Alexa647 using NHS chemistry (Molecular Probes ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells from each sample were labeled with TotalSeq™-B0251-0254 anti-human Hashtag Antibodies (clone LNH-94, Biolegend, RRID ...
-
bioRxiv - Immunology 2024Quote: ... 500ul of fresh cell culture media for each respective cell type were combined with 10 µl of 0.5ug/µl purified anti-human ICAM-1 blocking antibody (Clone HA58, BioLegend), added to the wells ...
-
bioRxiv - Immunology 2024Quote: Up to 20,000 cells per sample (Table S1) were stained with the TotalSeq-C Human Universal Cocktail v1 (BioLegend) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... they were stained with 1 uL each of the following TotalSeqC surface antibodies: anti-human IgM (BioLegend, Cat#314547), anti-human IgD (BioLegend ...
-
bioRxiv - Immunology 2024Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
-
bioRxiv - Immunology 2024Quote: To measure levels of 13 soluble factors simultaneously using the LEGENDplex Human CD8/NK Panel (BioLegend, San Diego, USA), supernatants were collected from cytotoxicity assays conducted in the Maestro Z instrument at endpoint and stored at -20°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... and live cells stained with APC anti-human GD2 (#357306; Biolegend) (isotype control, APC Mouse IgG2a, k; #400220, Biolegend) or Anti-human/mouse CD49f antibody (#313620 ...
-
bioRxiv - Immunology 2024Quote: ... in presence (stimulated condition) or absence (unstimulated condition) of 5 μg/ml anti-human CD3ε (clone OKT3; #317302, BioLegend) in PBS at 37°C for 2h ...
-
bioRxiv - Immunology 2024Quote: ... the supernatant was discarded and the cell pellet was resuspended in 50 µL of FACS Buffer (0.5% BSA, 2 mM EDTA in DPBS) with 1 µL of human TruStain Fc-block (1:50, Biolegend) and incubated for 15 minutes at 4 °C ...
-
bioRxiv - Microbiology 2024Quote: ... and TNF-α levels using the LEGENDplex™ Human Essential Immune Response Panel Mix and Match (Cat #740932, BioLegend) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: ... followed by another wash with PBS and staining with fluorescently conjugated monoclonal antibodies against APC conjugated anti-human CD56 (clone 5.1H11, Biolegend) for 20 minutes at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... + 5 ml FBS + 2mM Ethylenediaminetetraacetic acid [EDTA]) with Human TruStain FcX™ Fc Receptor Blocking Solution (1:20; BioLegend) for 15 minutes at room temperature in foil ...
-
bioRxiv - Immunology 2024Quote: ... as well as stained with activation marks Brilliant Violet 421-labeled anti-human CD38 mAb (Clone HIT2, BioLegend, USA), APC-labeled anti-human HLA-DR mAb (Clone L243 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Pluripotency marker expression was assessed every 10 passages by flow cytometry (PE anti-human SSEA-4 antibody, BioLegend, #330405) or RT-qPCR for DNMT3B ...
-
bioRxiv - Cell Biology 2024Quote: The Chemokine concentrations in cell culture media were assessed using the 13-plex LEGENDplex Human Inflammation Panel 1 (Biolegend), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... MOLM-13 cells were counted and then blocked as per the manufacturer’s protocol with Human TruStain FcX (Fc block, BioLegend) for 15 minutes on ice before labeling ...
-
bioRxiv - Molecular Biology 2024Quote: ... The last four dilutions were used for ELISA with Legend Max Human alpha-synuclein (colormetric) ELISA kit (Biolegend Inc.) according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: ... BD Biosciences (human CD45-PE, clone HI30, 1:200, mouse CD45-APC-Cy7, clone 30-F11, 1:100, BioLegend). Upon confirmation of engraftment ...
-
bioRxiv - Cancer Biology 2024Quote: ... the stained samples were then optionally incubated and stained with Alexa Fluor® 700 anti-human CD45 Antibody (BioLegend). Post staining ...
-
bioRxiv - Microbiology 2024Quote: ... parasites were washed three times in PBS and stained with 1:50 anti-human IgG APC (Biolegend, cat# 409306) and 1:2000 SYBR green (Invitrogen cat # S7563 ...
-
bioRxiv - Bioengineering 2024Quote: Jurkat cell activation was induced using Human (0.5 µg/ml) T-Activator anti-CD3 and anti-CD28 antibodies (BioLegend) for cell expansion and activation.
-
bioRxiv - Immunology 2024Quote: ... Antigen-reactive CD8+ cells were identified by co-expression of NFAT-mCherry and anti-human CD69 PE (BioLegend; FN50).
-
bioRxiv - Genomics 2024Quote: Single-cell suspension obtained from the tumor tissue were stained with anti-CD45 (PerCP/Cyanine5.5 anti-human CD45 Antibody, Biolegend) and anti-CD3 (PE/Cyanine7 anti-human CD3 Antibody ...
-
bioRxiv - Immunology 2024Quote: ... Cells were washed in 2% FBS 2 mM EDTA-PBS and surface stained with the following anti-human antibodies: CD45 (PerCP/Cyanine5.5, 2D1, Biolegend), CD137 (BV650 ...
-
bioRxiv - Immunology 2024Quote: ... and individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...
-
bioRxiv - Immunology 2024Quote: ... individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...
-
bioRxiv - Immunology 2024Quote: ... resuspended in 100ul of PBS with 10% FBS and 2 μl of anti-human CD3 AF488 (clone UCHT1, Biolegend), 2 μl of anti-human CD14 BV480 (clone M5E2 ...
-
bioRxiv - Genomics 2024Quote: Resuspend the isolated PBMCs in the recommended buffer provided with the MojoSort Human CD4 T Cell Isolation Kit (Biolegend). Add a biotin-conjugated antibody cocktail specific for non-CD4+ cells (e.g. ...
-
bioRxiv - Immunology 2024Quote: Up to 2 million hashtagged and fluorescently sorted naïve CD4+ T cells were stained with Mouse or Human TotalSeq™-C Universal Cocktail V1.0 CITEseq antibodies (Biolegend) according to manufacturer directions ...
-
bioRxiv - Immunology 2024Quote: ... followed by incubation with the secondary antibody PE F(ab’)2 Goat anti-human IgG Fcγ (BioLegend, Cat 398004) for 1h at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... CXCL10 in supernatant was measured using the ELISA MAX™ Deluxe Set Human CXCL10 (IP-10) kit (Biolegend, 439904). OD450nm and OD570 as background were measured using Multiskan SkyHigh plate reader (Thermo ScientificTM).
-
bioRxiv - Immunology 2024Quote: ELISA targeting IFN-γ were conducted using the ELISA MAX™ Standard Set Human IFN-γ (Biolegend, California, USA). The procedure was done according to the manufacturers protocol with slight alterations ...
-
bioRxiv - Immunology 2024Quote: ... about 3-4 million CD8+ T cells were blocked with Fc receptor blocking solution (Human TruStain FcX, BioLegend, #422302) for 10 minutes on ice and stained with BEAM-T assemblies for 15 minutes on ice ...
-
bioRxiv - Immunology 2024Quote: ... The cells were also stained with Invitrogen’s near-IR fluorescent reactive dye and an anti-human CD3 dye (UCHT1 clone) from BioLegend. Stained cells were analyzed using a FACSymphony benchtop analyzer ...
-
bioRxiv - Bioengineering 2024Quote: ... samples were washed twice with flow buffer (HBSS with 0.25% BSA) and stained with an Alexa Fluor 647-conjugated anti-human CD8 antibody (BioLegend) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2024Quote: ... CD8+ T cells were stained overnight at 4°C using Alexa Fluor 647-conjugated anti-human CD8 antibody (BioLegend). Confocal imaging was performed using an inverted laser scanning confocal microscope ...
-
bioRxiv - Bioengineering 2024Quote: ... streptavidin-conjugated MBs were washed once by centrifugation and incubated with 6 µg biotin anti-human αvβ3 antibody (304412, BioLegend) on ice for 30 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... BM aspiration were performed and analyzed by flow cytometry for the human cell engraftment utilizing mice-CD45 FITC (Biolegend), and human CD45 PerCPCy5.5 (Biolegend) ...
-
bioRxiv - Systems Biology 2021Quote: ... surface proteins were stained with a cocktail of 53 TotalSeq-C antibodies (Biolegend). Antibody concentrations were either predetermined by titration (Kotliarov et al. ...
-
bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following cell surface proteins were purchased from Biolegend with different fluorochrome labels ...