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Citations for BioLegend :
2601 - 2650 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... In order to measure secreted IL-1β we used the commercial Human IL-1β ELISA MAX kit (Biolegend), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: ... permeabilized for 10 min in PBS/0.1% saponin and stained with FITC-conjugated anti-human Perforin (γG9, Biolegend) and PE-conjugated anti-human Perforin antibody (B-D 48 ...
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Bioengineering 2022Quote: ... Enriched CD34+ HSPCs were stained with APC anti-human CD34 antibodies (clone 581, Biolegend, San Jose, CA, USA), and sample purity was assessed on an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: Secreted cytokines were assessed with LEGENDplex Human Th Panel 12-plex and analysed with Qognit software (both BioLegend). For cytokine secretion by Tregs ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the extracellular staining according to the manufacturer’s protocol: PerCP/Cyanine5.5 anti-human CD3 Antibody (#300430, BioLegend), Brilliant Violet 421 anti-human CD4 Antibody (#357424 ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Neuroscience 2024Quote: Cytokine levels in saliva were measured twice per sample using the LEGENDplex Human Essential Immune Response panel (BioLegend), as described in previous studies 26,57 ...
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bioRxiv - Immunology 2024Quote: ... and 1 mL 400 mg/mL Pacific Blue-mouse-anti-human CD45 (BioLegend; Cat. No. 304029; clone HI30), 1 mL 50 mg/mL PE/Dazzle594-mouse-anti-human CD16 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and 1 mL 70 mg/mL BV421-mouse-anti-human CD103 (Biolegend; Cat. No. 350214; clone Ber-ACT8). After the incubation ...
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bioRxiv - Immunology 2024Quote: ... 100 µL 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) was added to each well ...
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bioRxiv - Microbiology 2024Quote: ... Activation of MAPK and NF-κB signaling pathways was triggered using human recombinant TNF-α (BioLegend, Cat. 570102). Detailed information concerning experimental procedure is given in supplementary material.
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bioRxiv - Immunology 2023Quote: ... The amount of secreted IFN-γ was determined the LEGEND MAX™ Human IFN-γ ELISA Kit (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... untreated AML patients were determined by enzyme-linked immunosorbent assay (ELISA) using an IL21 Human ELISA kit (Biolegend), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: Determination of human α-synuclein concentration in nodose ganglia was performed according to manufacter’s instructions (Biolegend, Cat#844101). The 1:10 diluted samples were run in triplicates and reference standard curve ranged from 6.1 – 1500 pg per mL ...
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bioRxiv - Microbiology 2023Quote: ... fluorescein isothiocyanate-conjugated polyclonal goat anti-human C3b antibody (ICN) and PE-conjugated goat anti-mouse IgG (Biolegend). For the neutrophil killing assays ...
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bioRxiv - Immunology 2023Quote: Cytokines were quantified using the LEGENDplex Human CD8/NK Panel 13-plex (Cat.No # 740267 Lot B330268) from Biolegend. This is a bead-based immunoassay able to quantify the concentration of different analytes in co-culture supernatants ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of human cellsurface molecules: ①Purchased from Biolegend(USA): Anti-hCD36-PE(clone 5271).
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bioRxiv - Immunology 2023Quote: ... 100 µl 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) was added to each well ...
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bioRxiv - Immunology 2023Quote: Cytokine analysis from co-culture supernatants was performed with Human CD8/NK Panel (13-plex) Cat#741065 (BioLegend) following the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Dynabeads M270-Epoxy (Thermo) was covalently conjugated with combinations of mouse anti-human CD3 antibody (clone UCHT1, BioLegend), mouse anti-human CD28 antibody (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 720-IC-200) or 1 μg/ml Fc-VCAM-1 (Recombinant Human VCAM-1-Fc Chimera, Biolegend, 553706) was added to the chamber slide ...
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bioRxiv - Bioengineering 2024Quote: ... TCR was detected using an anti-human TCR α/β antibody conjugate to BV421 (Biolegend, San Diego, CA). Flow cytometry to assess CAR and TCR positivity was performed on Day 8 of manufacturing on an Attune NxT flow cytometer (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... 5 μl PE-labeled mouse anti-human CD11b fluorescent antibody (cat. no. 301306; 1:1,000 dilution; BioLegend, Inc.) or FITC-labeled mouse anti human CD14 fluorescent antibody (cat ...
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bioRxiv - Neuroscience 2024Quote: ... Macrophages were instead stained with 5 ul of either APC/Cy7-anti human CD16 antibody clone 3G8 (Biolegend), APC-anti human CD206 antibody (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted at 1X concentration for 30 min on ice with a master mix of a combination of primary antibodies (E-cadherin PE/Cy7 Biolegend, Clone 67A4 ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted at 1X concentration for 30 min on ice with a master mix of a combination of primary antibodies (E-cadherin PE/Cy7 Biolegend, Clone 67A4, 324 115; CD10 BV421, BioLegend, Clone HI10a ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4□ overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302), chicken anti-GFAP (1:300 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
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bioRxiv - Neuroscience 2020Quote: Free floating 30 μm sagittal brain sections were treated for antigen retrieval using sodium citrate buffer and subsequently stained using primary antibodies for Iba1 (1:250, rabbit, Wako) and anti-6E10 (1:1000, Biolegend), and the corresponding secondary antibodies and nuclear counterstain using DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... samples have been incubated overnight at 4°C with primary antibody anti β-amyloid (Aβ) (clone 6E10, 1:500, BioLegend) diluted in blocking buffer and stained with the corresponding goat secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel Parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... and 200 μl of primary antibody (1:200 anti-αSMA antibody, eBioscience, clone 1A4, efluor 570; 1:200 anti-CD31 antibody, Biolegend, clone MEC13.3 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% Sodium Azide and with one of the following primary antibody dilutions: Mouse Anti-HA (1:2000; HA.11, Biolegend), Mouse Anti-V5 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then blocked (PBS-0.25% Triton X-100+ 1% BSA (bovine serum albumin)) for 1h at RT and then primary antibodies (anti-βIII tubulin (Tuj1, 1:1000, BioLegend), anti-NFH (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked with 5% bovine serum albumin (BSA) and 5% goat serum for 1hr at RT and incubated with primary antibodies (anti-βIII-tubulin antibody [BioLegend]) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... isolated neutrophils were labeled with a panel of primary antibodies (anti-CD3, anti-CD45, anti-CD19, anti-CD14, anti-CD66b, anti-CD15 from Biolegend) for 20 minutes at room temperature and washed twice ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Cancer Biology 2021Quote: ... The following fluorophore-conjugated primary antibodies were used in flow cytometry studies: Alexa Fluor® 488 antimouse CD45 (BioLegend #103122), Alexa Fluor® 594 anti-mouse CD3 (BioLegend #100240) ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with combinations of the following primary antibodies in permeabilization buffer for 30 min at room temperature: Helios (22F6, BioLegend), T-bet (4B10 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5mM EDTA and 1% BSA) and incubated with the following primary antibodies: anti-mouse CD45 (Brilliant Violet 570, 30-F11, 1:200, Biolegend), anti-mouse Fce-R1 alpha (PE-Cyanine7 ...
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bioRxiv - Developmental Biology 2019Quote: ... digested enzymatically with a papain-based solution (Sumitomo Bakelite) and then resuspended in 0.3% BSA/PBS containing primary antibodies (PE-conjugated CD133 (mouse, 1:500, BioLegend, 141204) and APC-conjugated CD24 (mouse ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation for 1h at RT in a 1:1000 dilution in blocking buffer of primary Ab (purified anti-Filaggrin polyclonal Ab, clone Poly19058, Biolegend). After washing ...
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bioRxiv - Immunology 2021Quote: ... Primary bone marrow-derived murine macrophages (BMDMs) were pretreated with PBS or murine IL-27 (50 ng/ml from BioLegend) for 24 hours and then infected with S ...