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Citations for BioLegend :
2601 - 2650 of 8491 citations for 6 4 METHOXY PHENYL 2 PHENYL PYRAZOLO 1 5 A PYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Sections were incubated with the following primary antibodies at the indicated dilution in blocking buffer overnight at 4°C: NeuN (BioLegend 1B7, cat #834502), p65 (BioLegend Poly6226 ...
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bioRxiv - Immunology 2024Quote: ... Surface proteins were then stained at 4°C for 30 minutes using the following markers: NIR Zombie dye APC-Cy7 (Biolegend, Cat No. 423105), CD20 PE (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... peripheral blood or BM were incubated for 20 min at 4°C with the following anti-mouse conjugated antibodies: anti-CD45-PerCP/Cy5.5 (BioLegend, clone 30-F11, #103132), anti-CD45.1-PE/Cyanine7 (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Systems Biology 2019Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) and incubated overnight with primary anti-XBP1s antibody (Biolegend Poly6195) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane was blocked in 5% milk followed by probing with either α-HA Mouse Monoclonal (Catalogue #HA.11; Biolegend, SanDiego, CA) or α-Rsp5 Rabbit Polyclonal [84] antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: ... The suspensions were then spun at 300 x g for 5 minutes and resuspended in red blood cell (RBC) lysis buffer (Biolegend, 420302) for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membrane was next added to 5% nonfat milk in TBST containing horseradish peroxidase-conjugate goat anti-mouse IgG1 secondary antibodies (Biolegend 405306) diluted at 1:5000 and incubated on a horizontal rocker for 60 minutes ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2020Quote: ... cells were pelleted for 5 minutes at 500xg and resuspended in 50 µL Intracellular Staining Perm Wash Buffer supplemented with 5 µL of TruStain FcX (Biolegend, 422302) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue sections were treated with blocking solution (5% normal donkey serum in PBS) and then stained with FITC-conjugated anti-Ly6G antibody (BioLegend, 1A8). Nuclei were stained by DAPI ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Bioengineering 2024Quote: Resting T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 µL/well of the antibody cocktail for extracellular staining: anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200) ...
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bioRxiv - Biophysics 2023Quote: ... The gel substrate for T cells had a Young’s modulus of 1.2 kPa and was coated with 5 μg/mL each of anti-CD3 (OKT3; 317326; BioLegend, CA, USA) and anti-CD28 (CD28.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... Collected cell populations were immediately seeded in tissue culture at a concentration of 2x105 cells per 1.9 cm2 in DMEM containing 5% FBS and 10 ng/mL TGFβ (Biolegend Cat 763102) or 500 nM Prostaglandin F (Cayman Chemical Cat 16010).
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellets were harvested and resuspended in 5 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 5 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... washed cells were resuspended in 100 μL of FACS buffer with 5 μL of PE anti-human TMPRSS2 antibody (BioLegend #378403) per million cells and incubated for 30 min at 4 °C in the dark ...
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bioRxiv - Immunology 2022Quote: ... CD44 – PE-Cyanine7 (eBiosciences, # 25-0441-81; 1:600), CD4 – APC (eBiosciences, #17-0042-83; 1:200) and CD8a – APC-Cyanine7 (BioLegend, #100714; 1:400) combined with AZDye 488 alkyne/azide ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 cells/sample were Fc-blocked with TruStain fcX (BioLegend, #101320, 1/200) for 10 minutes at room temperature followed by LIVE/DEAD Fixable Aqua (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... GcrA (1:1,000 dilution, rabbit)36and RpoD (1:250 dilution, Mouse IgG2b) (Neoclone, BioLegend) antibodies were used ...
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bioRxiv - Neuroscience 2020Quote: ... and purified Alex fluor 488 anti-β-Amyloid 1-16 antibody (1:200, BioLegend) was used to visualize amyloid plaques.
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bioRxiv - Biochemistry 2022Quote: ... and monoclonal antibodies 4G8 to Aβ17-24 (BioLegend, 800702, IP: 1:500–1:2,500), Penta-His Antibody (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... Antibodies for blocking LFA-1 - ICAM-1 interaction were anti-ICAM1 (clone HA58, Biolegend) and anti-LFA-1 (clone TS1/18 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μL anti-CD4 BV605 (OKT4) (BioLegend, cat # 317438, used at 1:25 dilution), 0.5 μL anti-CD3 Percp (SK7 ...
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bioRxiv - Genomics 2022Quote: ... 1:400 dilution of Anti-Mouse Sca-1 PB antibody (Biolegend, Cat#: E13-161.7), 1:200 dilution of Anti-Mouse cKit APC antibody (BD Biosciences ...
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bioRxiv - Genomics 2022Quote: ... 1:200 dilution of Anti-Mouse Gr-1 APC-Cy7 antibody (Biolegend, Cat#: 108423) and 1:1000 dilution of Anti-Mouse Mac1 PE antibody (Biolegend ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×106 CD34+ HSPCs were stained with APC-Cy7-anti-CD34 (1:100) (BioLegend), PE-Cy7-anti-CD38 (1:50) ...
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bioRxiv - Immunology 2020Quote: ... anti-CD3e Biotin (1:400, 145-2C11, eBioscience) with Streptavidin BV510 (1:1000, Biolegend), anti-CD45 PB (3ug per mouse anti-CD4 BV605 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with 6E10 anti-amyloid-beta 1-16 mouse IgG1 (1:750, Biolegend, 803001) in 0.1% w/v Triton-X100 containing TBS at 4°C for 24 h ...
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bioRxiv - Immunology 2022Quote: ... BV605 anti-CD11b (1:500) and PE anti-F4/80 (1:200) (All BioLegend). KCs were gated as the CD45+CD31-CD11blowF4/80+ population using FACSAria SORP (BD Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: anti-CD271 antibodies (1:100, MA5-13311, Thermo Fisher Scientific, 1:100, 839701, BioLegend), anti-Foxd3 antibody (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were stained with anti-Ab (H31L21, Invitrogen, 1:1000, 6Ε10, BioLegend, 1:1000) to label plaques ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-β-amyloid 1-16 (6E10, mouse IgG1 1:100 BioLegend, #SIG-39320) to generate “Aβ negative” s-extract ...
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bioRxiv - Immunology 2023Quote: ... at 1:20,000 for 1 h before development with TMB Substrate Set (421101, Biolegend). The chromogenic reaction was stopped with 1 M HCL ...
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bioRxiv - Cancer Biology 2022Quote: ... The lymphoid panel consisted of antibodies directed against PD-1 (Biolegend #135241; 1:250), FoxP3 (Biolegend #126419 ...
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bioRxiv - Cell Biology 2023Quote: ... and β1-integrin clone HM β1-1 Armenian hamster mAb (1:50, 102202, Biolegend). Secondary antibodies were conjugated to Alexa Fluor 405 ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-Beta tubulin III (Tuj1) (1:500; Neuromics, CH23005 & 1:1000; Biolegend, 801201). The tNeurons were washed with PBS three times and incubated with fluorophore-conjugated secondary antibody solution in the dark for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 1× PBS/1% FBS and blocked with CD16/32 (Biolegend). After 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Extracellular staining was performed in FACS buffer (PBS + 2% FBS + 2mM EDTA) with the following antibodies: CD4 (GK1.5, Biolegend), CD8 (53-6.7 ...