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Citations for BioLegend :
2551 - 2600 of 2921 citations for Recombinant Human GLB1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were labeled with live/dead dye (Zombie Aqua) for 10 min at RT and blocked with human TruStain FcX (BioLegend) for 20 min at RT ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T lymphocytes were purified (> 98%) by negative selection with MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3 ...
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bioRxiv - Biochemistry 2021Quote: ... with remaining cells washed and resuspended in 1x PBS with 1% BSA and stained for 15 minutes with the following antibodies: human CD45-APC (Clone 2D1) (Biolegend), human CD33-PE-Cy7 (Clone P67.6 ...
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bioRxiv - Genomics 2021Quote: ... and then up to 500,000 cells from each condition were stained with a unique TotalSeq™-A anti-human Hashtag antibody (BioLegend) in 50 ul for 30 minutes at 4°C on a Laminar Wash™ 16-well strip (Curiox Biosystems) ...
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin) with mouse anti-human monoclonal antibody CD62P-AlexaFluor647 (Clone AK4, Cat.No. 304918, BioLegend, USA). and activation associated conformational change in integrin αIIbβ3 was detected with a mouse anti-human monoclonal antibody PAC1-FITC (Clone PAC-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... while the MDR1 replicons were stained with 5 μL of PE anti-human CD243 (ABCB1) antibody clone 4E3.16 (Biolegend, US). After staining for 30 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... Immunostaining of specific receptors was performed by incubating cells overnight with AlexaFluor 647 conjugated primary antibodies (human CD4: OKT4, dilution: 1:100, source: Biolegend; human CD45 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells suspensions of 100 μL were incubated for 15 min at RT with 2 μL Human TruStain FcX (Biolegend, 422301) to prevent nonspecific Fc receptor binding ...
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bioRxiv - Cancer Biology 2021Quote: ... PE anti-human TRAILR4 (DcR2) (R&DSystems, Clone 104918) or PE Mouse IgG1 κ Isotype Control (Biolegend, Clone MOPC-21) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the in vivo samples to obtain absolute counts of events acquired on the cytometer as well as using human and mouse Fc blocking antibodies (Biolegend) to reduce non-specific antibody staining ...
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bioRxiv - Bioengineering 2022Quote: ... and Phycoerythrin-conjugated Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody (Fisher) for ACE2-Bite and APC anti-human IgG Fc Antibody clone HP6017 (Biolegend) for ACE2-Fc were added to the wells and incubated for another hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Binding Buffer containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Immunology 2022Quote: ... Cell samples at each timepoint were prepared as described and co-stained with PE-anti-CD19 and allophycocyanin-conjugated mouse anti-human CD23 (APC-anti-CD23, BioLegend, clone EBVCS-5 ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... cells in the plasma layer were collected to isolate CD14+ monocytes using a commercial kit (MojoSort human CD14+ monocytes isolation kit, 480047, Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... the cell pellet was resuspended in 100 μl of Alexa Fluor 647-conjugated anti-human/mouse SSEA1 antibody (catalog no. MC-480; BioLegend) diluted 1:100 in PBS + 2.5% FBS and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2022Quote: ... and the DAXL-88 (anti-AXL) binding assay used Alexa Fluor 647 AffiniPure anti-Fc tag Goat Anti-Human IgG (410711, BioLegend). Anti-Human CD4 antibody (BD ...
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bioRxiv - Immunology 2022Quote: ... Surface staining was performed with anti-mouse Fc receptor antibody (clone 2.4G2, UCSF Hybridoma Core) or Human TruStain FcX (BioLegend #422302) in PBS with 2% FCS for 30 min on ice ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with ice-cold FACS buffer and incubated with 2 μg/mL PE anti-human IgG Fc (BioLegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed once with ice-cold FACS buffer and incubated with 1 μg/mL PE anti-human IgG Fc (Biolegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were sorted by fluorescent assisted cell sorting on the SH800S cell sorter (Sony Biotechnology) using the following antibodies: FITC anti-human lineage cocktail (Cat# 343604, BioLegend) and APC anti-human CD34 (Cat# 343509 ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... hPGCLCs were dissociated into single cells and then stained with Alexa Fluor 647-conjugated rat anti-human PDPN (337007, BioLegend) and BV421-conjugated anti-CD49f (INTEGRINα6 ...
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bioRxiv - Immunology 2022Quote: ... Subsequent iterative cycles of standard immunohistochemistry were performed using the following anti-human primary antibodies: podoplanin (D240, BioLegend; 1:100), LYVE-1 (rabbit polyclonal ...
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bioRxiv - Immunology 2019Quote: ... the cells were thawed and transferred to a 96-well plate and stained for viability with Viability Dye eFluor 506 (eBioscience) followed by incubation with the surface antibodies: anti-human CD3 AF700 (clone UCHT1, Biolegend), CD4 BV785 (clone OKT4 ...
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bioRxiv - Immunology 2019Quote: ... cells were sorted from PBMC using a BD FACSAria cell sorter after staining with anti-human CD4 PE-conjugated antibody (Cat. #317410, Biolegend) or separated by human CD4+ T cell isolation kit (Cat ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1-2 million HL-60 and THP-1 cells were resuspended in PBS containing 1% fetal bovine serum and stained with PE anti-human CD11b antibody (Biolegend). Stained cells were analyzed on FACScalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2019Quote: ... Expression of the chimeric receptor was determined by staining with AlexaFlour 647-conjugated anti-3S193 idiotype [supplied by Ludwig Institute for Cancer Research (LICR)] and truncated CD34 was detected with PE-conjugated anti-human CD34 (BioLegend). Cell surface phenotyping of transduced cells was determined by staining with BV785-conjugated anti-human CD3 (UCHT1 ...
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bioRxiv - Immunology 2019Quote: ... GolgiPlug™ and GolgiStop™ were added 3 h before cells were processed for surface and intracellular staining for CD4 (BV785-conjugated anti-human; OKT-4, BioLegend), CD8 (APC-H7-conjugated anti-human ...
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... phycoerythrin (PE)-labeled mouse anti-human CD41a ab was used to gate platelets and FITC-labeled monoclonal P2RY12 antibody (anti-P2Y12, Biolegend) was used to detect surface expression of platelet P2Y12.
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:100 dilution of biotin anti-human IgG Fc antibody (clone HP6017, BioLegend) for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088 ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were collected and the amount of IL10 in the supernatants were measured using the Human IL10 ELISA MAX (Biolegend) kit according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... for 8 hours and then surface staining was performed using the following abs anti-human BDCA-2-PE (clone AC144; Biolegend), CD123-PeCy7 (clone 6H6 ...
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bioRxiv - Immunology 2020Quote: ... then cells were washed with 2% FCS-PBS and surface-stained with anti-human HLA-DR-Pacific Blue or -APC (clone L243; Biolegend) and anti-human CD123-FITC or -PeCy5 (clone 6H6 ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed with FACS buffer and labeled with PE conjugated mouse anti-human TNFRSF12A antibody (clone ITEM-4, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of PE-conjugated mouse anti-human IgG Fc antibody (BioLegend) on ice in dark for 1h ...
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bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...