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2551 - 2600 of 5512 citations for Recombinant Human APCS Protein since 2019

• CBFA2T3-GLIS2-dependent pediatric acute megakaryoblastic leukemia is driven by GLIS2 and sensitive to Navitoclax

  Mathieu Neault, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Cell death was measured using the annexin V-APC Apoptosis Detection Kit (BioLegend) according to the manufacturer’s instructions and analysed with a BD Fortessa X20 (BD ...
• Microglial cannabinoid receptor type 1 mediates social memory deficits produced by adolescent THC exposure and 16p11.2 duplication

  Yuto Hasegawa, et al., bioRxiv - Neuroscience 2023

  Quote: ... APC rat anti-mouse P2ry12 (cat # 848006, BioLegend, clone S16007D, 2 ng/μl), BV421 rat anti-mouse CD45 (cat # 103133 ...
• The renal capsule, a vibrant and adaptive cell environment of the kidney in homeostasis and aging

  Ben Korin, et al., bioRxiv - Immunology 2023

  Quote: ... injection of APC conjugated anti-mouse CD45 (cat# 103112; clone 30-F11; Biolegend) at 10 µg per mouse ...
• The CD4 transmembrane GGXXG and juxtamembrane (C/F)CV+C motifs mediate pMHCII-specific signaling independently of CD4-LCK interactions

  Mark S. Lee, et al., bioRxiv - Immunology 2023

  Quote: ... Tubes were then stained using 1μL anti-CD4 (APC conjugate; clone GK1.5, BioLegend), 1.5μL anti-LCK (PE conjugate ...
• Haematopoietic stem cell numbers are not solely determined by niche availability

  Shoichiro Takeishi, et al., bioRxiv - Cell Biology 2023

  Quote: ... F4/80-PE (BM8; 123110) and anti-CD45.2-APC (104; 109814) from BioLegend, and anti-CD3e-PerCP-Cyanine5.5 (145-2C11 ...
• Droplet based low input proteomic platform for rare cell populations

  Matthew Waas, et al., bioRxiv - Biochemistry 2023

  Quote: ... APC rat anti-mouse CD326 clone G8.8 (EpCAM; BioLegend, 118214; 1:200 dilution) and PE Mouse Anti-Mouse CD36 clone CRF D-2712 (BD Biosciences ...
• A human prenatal skin cell atlas reveals immune cell regulation of skin morphogenesis

  Nusayhah Hudaa Gopee, et al., bioRxiv - Developmental Biology 2023

  Quote: ... additional staining with anti-CD34 (4 µL CD34 APC/Cy7, clone: 581, Biolegend) and anti-CD14 antibodies (3 µL CD14 PE-CF594 ...
• Genome-wide CRISPR/Cas9 screen identifies MAT2A as a critical host factor for BK Polyomavirus

  Laura Caller, et al., bioRxiv - Microbiology 2023

  Quote: The primary antibodies used were APC conjugated MHC-I (BioLegend Cat# 311410, RRID:AB_314879), mouse anti-Cas9 (Santa Cruz Cat# sc-517386 ...
• Self-Assembled Protein Vesicles as Vaccine Delivery Platform to Enhance Antigen-Specific Immune Responses

  Yirui Li, Julie A. Champion, bioRxiv - Bioengineering 2024

  Quote: ... FITC anti-mouse CD8a antibody and APC/Cyanine7 anti-mouse CD4 antibody (BioLegend) in PBS ...
• A TLR7 Agonist Conjugated to a Nanofibrous Peptide Hydrogel as a Potent Vaccine Adjuvant

  Erin M. Euliano, et al., bioRxiv - Bioengineering 2024

  Quote: ... the only stain used was anti-mouse H-2Kb conjugated to APC (BioLegend). The samples were incubated on ice in the dark for 45 min before 1 mL of cold PBS was added and the samples were centrifuged at 200 rcf for 7 min at 4 °C ...
• Impaired Myofibroblast Proliferation is a Central Feature of Pathologic Post-Natal Alveolar Simplification

  Imran S. Khan, et al., bioRxiv - Physiology 2023

  Quote: ... anti-CD45 (clone 30F-11, BV786, APC/Cy7, PE/Cy7; BioLegend, BD Biosciences), anti-Epcam (clone G8.8 ...
• Social Determinants modulate NK cell activity via obesity, LDL, and DUSP1 signaling

  Yvonne Baumer, et al., bioRxiv - Immunology 2023

  Quote: ... CD56 and CD107a stained using anti-CD3-APC/Cy7 (SK7 clone, BioLegend, USA), anti-CD56-BV450 (NCAM16.2 clone ...
• Heparan sulphate binding controls in vivo half-life of the HpARI protein family

  Florent Colomb, et al., bioRxiv - Immunology 2024

  Quote: ... ILC2 markers: CD45 (AF700, clone 30-F11, or APC-Cy7, I3/2.3; Biolegend), CD90.2 (AF700 ...
• Heparan sulphate binding controls in vivo half-life of the HpARI protein family

  Florent Colomb, et al., bioRxiv - Immunology 2024

  Quote: ... Eosinophil markers: CD45 (AF700, clone 30-F11, or APC-Cy7, I3/2.3; Biolegend), SiglecF (PE ...
• Engineering CAR-NK cells to secrete IL15 sustains their anti-AML functionality, but is associated with systemic toxicities

  Ilias Christodoulou, et al., bioRxiv - Immunology 2021

  Quote: ... Human Fc receptors (FcRs) were blocked using Human TruStain FcX™ (BioLegend). In samples stained with multiple BD Horizon Brilliant reagents ...
• Proliferating CLL cells express high levels of CXCR4 and CD5

  Daniel Friedman, et al., bioRxiv - Cancer Biology 2023

  Quote: Human PBMCs were treated with Fc blocking solution (Human TruStain FcX, Biolegend) and stained with the following fluorescent antibodies from Biolegend (unless otherwise stated) ...
• The connection between Rap1 and Talin1 in CD4⁺ T Lymphocytes

  Frederic Lagarrigue, et al., bioRxiv - Cell Biology 2021

  Quote: ... Recombinant mouse ICAM-1 and VCAM-1-Fc were from BioLegend. Recombinant mouse MAdCAM-1 was from R&D Systems.
• Sustained mechanical tension governs fibrogenic activation of tendon stromal cells in systemic sclerosis

  Amro A. Hussien, et al., bioRxiv - Cell Biology 2021

  Quote: ... the cells were treated with recombinant TGF-β1 (580702, BioLegend®), as specified in figure legends ...
• GFAP-directed Inactivation of Men1 Exploits Glial Cell Plasticity in Favor of Neuroendocrine Reprogramming

  Suzann Duan, et al., bioRxiv - Cancer Biology 2022

  Quote: ... 30 ng/mL mouse recombinant FGF-10 (Biolegend, San Diego, CA), and 30 ng/mL recombinant human EGF (R&D Systems ...
• ONECUT2 restricts Microfold cell numbers in the small intestine; a multi-omics study

  Maria V. Luna Velez, et al., bioRxiv - Molecular Biology 2022

  Quote: ... treatment with 100 ng/mL of recombinant mouse TRANCE (RANKL) (BioLegend) was done for 6 or 9 days ...
• Connecting secretome to hematopoietic stem cell phenotype shifts in an engineered bone marrow niche

  Aidan E. Gilchrist, Brendan A.C. Harley, bioRxiv - Bioengineering 2020

  Quote: ... or none of the cytokines: recombinant murine TGFβ-1 (#763104, BioLegend), recombinant murine MMP-3 (#552704 ...
• Microglia protect fungi against copper starvation and promote brain infection

  Sally H. Mohamed, et al., bioRxiv - Immunology 2022

  Quote: ... media was further supplemented with 100ng/mL recombinant mouse IFNγ (Biolegend). After 24 hours ...
• A multi-enhancer hub at the Ets1 locus controls T cell differentiation and allergic inflammation through 3D genome topology

  Aditi Chandra, et al., bioRxiv - Genomics 2022

  Quote: ... and 20 ng/mL of recombinant IL-6 (BioLegend, Cat# 575702) and 1 μg/mL of soluble anti-CD28 for 6 days at 37°C ...
• Methylmalonic acid induces metabolic abnormalities and exhaustion in CD8⁺ T cells to suppress anti-tumor immunity

  Joanne D. Tejero, et al., bioRxiv - Immunology 2024

  Quote: ... and 10 ng/mL recombinant mouse interleukin-2 (IL-2) (BioLegend); additionally ...
• M2 macrophages dampen in utero sterile inflammation to prevent preterm birth and improve neonatal outcomes

  Valeria Garcia-Flores, et al., bioRxiv - Immunology 2024

  Quote: ... and 10 ng/ml of recombinant IL-13 (Cat# 575902, BioLegend). After 14-18 h ...
• Foxp3 depends on Ikaros for control of regulatory T cell gene expression and function

  Rajan M. Thomas, et al., bioRxiv - Immunology 2024

  Quote: ... was purchased from Bioxcell and recombinant mouse IL-2 (carrier free, cat # 575408 from Biolegend). IL-2/anti-IL-2 complexes were prepared by mixing both reagents ...
• Midbrain organoids with an SNCA gene triplication model key features of synucleinopathy

  Nguyen-Vi Mohamed, et al., bioRxiv - Neuroscience 2021

  Quote: ... Human TruStain FcXTM (Biolegend). Protected from light ...
• Single-cell transcriptional landscape of temporal neutrophil response to burn wound in larval zebrafish

  Yiran Hou, et al., bioRxiv - Immunology 2024

  Quote: ... Human TruStain FcX (BioLegend) was added at 1:20 to block Fc receptors for 10 minutes ...
• CITE-seq analysis reveals human cytomegalovirus and diabetes-associated adaptive NK cell alterations in cardiovascular disease

  Sujit Silas Armstrong, et al., bioRxiv - Immunology 2024

  Quote: ... human TGFβ1 (580706; BioLegend), human IL-12 (219-IL;R&D Sys-tems) ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD3 (BioLegend, #317324), mouse CD45 (BioLegend #103130) ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD45 (BioLegend #304014), human CD25 (BioLegend #302606) ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human FOXP3 (BioLegend #320116), Alexa Fluor® 647-conjugated anti-mouse integrin αvβ5 Ab (clone ALULA ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD45 (BioLegend #304014), Zombie NIR™ Fixable Viability Kit (BioLegend #423106 ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD25 (BioLegend #302606), human FOXP3 (BioLegend #320116) ...
• A pancreatic cancer mouse model with human immunity

  Norio Miyamura, et al., bioRxiv - Cancer Biology 2023

  Quote: ... human CD8 (BioLegend #344712), human CD45 (BioLegend #304014) ...
• Legionella pneumophila inhibits type I interferon signaling to avoid cell-intrinsic host cell defense.

  Charles N.S. Allen, et al., bioRxiv - Microbiology 2023

  Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
• Legionella pneumophila inhibits type I interferon signaling to avoid cell-intrinsic host cell defense.

  Charles N.S. Allen, et al., bioRxiv - Microbiology 2023

  Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
• SETD7-mediated lysine monomethylation is abundant on non-hyperphosphorylated nuclear Tau

  Maria Bichmann, et al., bioRxiv - Neuroscience 2020

  Quote: 250 μg protein from each human brain sample lysate was used for immunoprecipitation (IP) with a combination of Tau12 (Biolegend, cat. no. 806501), Tau 5 (Abcam ...
• Natural killer cell division regulates FcεRIγ expression downstream of mTOR activity

  Avishai Shemesh, et al., bioRxiv - Immunology 2021

  Quote: ... Human Fc receptors were blocked using human TruStain FcXTM (BioLegend Cat# 422302, RRID:AB_2818986).
• Frequent Aneuploidy in Primary Human T Cells Following CRISPR-Cas9 cleavage

  A.D. Nahmad, et al., bioRxiv - Bioengineering 2021

  Quote: ... containing 1/100 diluted anti-human CD3 or anti-human TCRα/β (Biolegend), both targeting the TCR complex ...
• Siglec-5 is an inhibitory immune checkpoint molecule for human T cells

  Aleksandra Vuchkovska, et al., bioRxiv - Immunology 2022

  Quote: ... Cells were blocked with human Fc receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes on ice ...
• Siglec-5 is an inhibitory immune checkpoint molecule for human T cells

  Aleksandra Vuchkovska, et al., bioRxiv - Immunology 2022

  Quote: ... A549 were blocked with human Fc receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes on ice ...
• A single episode of CMV reactivation initiates long-term expansion and differentiation of the NK cell repertoire

  Norfarazieda Hassan, et al., bioRxiv - Immunology 2022

  Quote: ... anti-human CD57-PB (BioLegend, Cat#322316], anti-human CD16-V500 [BD Biosciences, Cat#561314], anti-human CD69-PE-Cy7 [BioLegend, Cat#310190]). 5×105 to 1×106 cells were re-suspended in residual buffer and antibody panels added and incubated on ice for 20 to 30 minutes ...
• Natural killer-like B cells promote Th17 cell differentiation and exacerbate rheumatoid arthritis

  Ping Wang, et al., bioRxiv - Immunology 2021

  Quote: ... the cells were collected and stained with APC-Fire750-anti- mouse CD4 antibody (Biolegend), then were detected by flow cytometry ...
• PD-L1:CD80 Heterodimer Triggers CD28 While Repressing Both PD-1 and CTLA4 Pathways

  Yunlong Zhao, et al., bioRxiv - Immunology 2019

  Quote: ... Cells were sorted with anti-CD80 APC and anti-CD86 BV421 (Biolegend, catalog 305425) staining.
• Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques

  Timothy N. Hoang, et al., bioRxiv - Immunology 2020

  Quote: ... and anti-CD4-APC-Cy7 (clone OKT4; 2.5 μL; cat. # 317418) all from Biolegend; anti-CD38-FITC (clone AT1 ...
• Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques

  Timothy N. Hoang, et al., bioRxiv - Immunology 2020

  Quote: ... and anti-CD123-APC-Fire750 (clone 315; 2.5 μL; cat. # 306042) all from Biolegend; anti-GranzymeB-PE-TexasRed (clone GB11 ...
• SunCatcher: Clonal Barcoding with qPCR-Based Detection Enables Functional Analysis of Live Cells and Generation of Custom Combinations of Cells for Research and Discovery

  Qiuchen Guo, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Cells were incubated with anti-EpCAM (Clone G8.8, APC-Cy7, 1:400 dilution, BioLegend), anti-MHC-I (clone ...
• The HIV latency reversal agent HODHBt enhances NK Cell effector and memory-like functions by increasing IL-15 mediated-STAT activation

  Amanda B. Macedo, et al., bioRxiv - Cell Biology 2021

  Quote: ... followed by intracellular staining with TNF-α-APC-Cy7 and IFN-γ-PECy7 (Biolegend) for 30 min at 4°C ...
• Analysis of Preplatelets and Their Barbell Platelet Derivatives by Imaging Flow Cytometry

  Sam Kemble, et al., bioRxiv - Cell Biology 2021

  Quote: ... PE or APC mouse IgG1(1/100, MOPC-21, BioLegend, 400112 and 400120 respectively) were used to quantify CD62p exposure ...