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Citations for BioLegend :
2551 - 2600 of 8531 citations for HCC 1 CCL14 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell surface marker expression in the SORE6+ and SORE6-compartments of MDA-MB-231 cells cultured in vitro or recovered from primary tumors was assessed by flow cytometry following staining with Brilliant Violet 421TM anti-human CD133 (Biolegend Cat#372807), or APC anti-human CD44 (Biolegend Cat#338805 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... with 1 × 106 cells/mL 5000 rad irradiated PBMC + 50 U/mL IL-2 + 10 ng/mL IL-15 (both from NCI BRB Preclinical Biologics Repository) + 0.1 μg/mL each of anti-CD3 (Ultra-LEAF purified anti-human CD3 antibody clone OKT3; BioLegend, San Diego, CA) and anti-CD28 (Ultra-LEAF purified Anti-human CD28 antibody clone 28.2 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were then evaluated for IFN-γ levels using the Legend Max ELISA kit with pre-coated plates (human IFN-γ) (Biolegend). Surface phenotype of cells that had been removed from the well was determined by flow cytometry using fluorochrome conjugated antibodies specific for 4-1BB or CD69.
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Immunology 2019Quote: ... according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 μg/mL anti-human RP105 antibody clone MHR73-11 (BioLegend Cat.# 312907).
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... P815 cells were used as target cells for human CTLs and pulsed for 1h at 37°C with 1µg/ml anti-CD3 antibody (clone UCHT1, Biolegend, Cat no. 300438). They were washed and resuspended at 1x105 cells/ml in killing assay media (RPMI without phenol red + 2% FCS ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... HLA-A2 positive PBMC samples were further stained with PE labelled tetramer (home-made) and APC labelled human CD8+ antibody (BioLegend Cat#344721), and acquired with flow cytometer FACS Canto (BD).
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice and resuspended in PBS containing 2% FBS before staining in the presence of Fc block (Miltenyi 130-059-901) with anti-human CD45 Alexa700 (clone HI30, BioLegend cat #304024) and anti-mouse CD45.1 PE-Cy7 (clone A20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF) 488 mouse anti-human BAX antibody (0.5 μg/mL, Biolegend, Cat. No. 633603) or AF488 Mouse IgG1 κ isotype control (5 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... and stained overnight with Alexa 488-conjugated Alexa Fluor® 488 anti-tubulin β3 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Cancer Biology 2023Quote: ... The percentage of viable leukemic cells was determined by staining with Brilliant Violet 421 anti-human CD19 (Biolegend, San Diego, CA, USA), FITC Annexin V (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then plated as described above and human IL-10 was measured in the cell supernatants by ELISA (Biolegend, Cat. # 430604).
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bioRxiv - Immunology 2023Quote: Freshly sorted CD8+ T lymphocytes of four healthy volunteers were labelled with anti-human Hashtag antibodies TotalSeq-C (Biolegend, San Diego, CA) and were pooled together ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Microbiology 2024Quote: ... moDCs were also stained for surface markers using BioLegend anti-human antibodies for monocyte and dendritic cell associated surface markers: CD14 (M5E2; BioLegend, Cat# 301814), CD11c (B-ly6 ...
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bioRxiv - Immunology 2024Quote: ... then treated with Human TruStain FcX for blocking Fc receptors and stained with Brilliant Violet (BV) 421 anti-CD3 (BioLegend, clone OKT3), APC anti-human CD7 (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor® 700 anti-human CD41 antibody and Zombie NIR® fixable dye were obtained from BioLegend (San Diego, CA, USA). Anti-human CD62P conjugated PE antibody was obtained from Beckman Coulter (Brea ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with the following antibody cocktail diluted in flow cytometry staining buffer: mouse anti-human CD3 BV750 (BioLegend Cat 344846), mouse anti-human CD4 AF700 (BioLegend Cat 300526) ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for purity: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD20 PerCP/Cy5.5 (BioLegend catalog #375510 ...
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bioRxiv - Molecular Biology 2024Quote: ... IL-6 and IL-15 was quantified in cell supernatants using a sandwich ELISA Max Deluxe Set Human (Biolegend, San Diego, CA), according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... Sca-1-PerCP-Cy5.5 (Biolegend, 1:1,000), CD45-APC-Cy7 (Biolegend,1:200) ...
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bioRxiv - Physiology 2022Quote: ... Mac-1 and Gr-1 (Biolegend, 133307), CD4 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... Icam-1 Antibody (Biolegend, 116101, 1:60), Anti-Cytokeratin19 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... PD-1(Biolegend, RMP1-30, 1:200), KLRG1(Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... CD106 (1:100, MVCAM.A, #105717), CD62L (1:200, MEL-14, #104435), PD-1 (1:100, 29F.1A12, #135215) (Biolegend); Streptavidin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 1:300), NK1.1 (108736, 1:150), Tbet (644810, 1:50), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, CD19 (45-0193-82 ...
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bioRxiv - Immunology 2023Quote: ... 1:100), GR1 (108428, 1:300), NK1.1 (108728, 1:200), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, IL-22 (12-7221-82 ...
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bioRxiv - Biophysics 2022Quote: ... coverslips of each batch were incubated with 30 µg/ml anti-human IgG Fc (cross-reacts with rabbit) conjugated to Alexa Fluor-488 (Cat# 409321; BioLegend, San Diego, CA) for 45 minutes at room temperature in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for the analysis of human synovial cells with cell sorting: APC anti-CD3 (Clone OKT3, BioLegend Cat# 317317, RRID:AB_1937213), PE anti-CD45RO (Clone UCHL1 ...
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bioRxiv - Bioengineering 2021Quote: ... Anti-FLAG epitope (DYKDDDDK, catalog# 637301) and horseradish peroxidase (HRP) Donkey anti-human IgG antibody (catalog #410902) were purchased from Biolegend (San Diego, CA). The secondary antibody anti-rat IgG HRP (catalog# 7077 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD4+ T cells were isolated from the PBMC population by immunomagnetic positive selection using the MojoSort Human CD4 Nanobeads (BioLegend, San Diego, CA) and an EasySep Magnet (STEMCELL™ Technologies Inc. ...
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bioRxiv - Neuroscience 2020Quote: 250 μg protein from each human brain sample lysate was used for immunoprecipitation (IP) with a combination of Tau12 (Biolegend, cat. no. 806501), Tau 5 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... We aspirated pelleted barcoded cells to a volume of 50 µL and incubated them with 15 µL of Human TruStain FcX (Biolegend, San Diego, CA) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from triplicate wells of each condition were collected at 24 hrs and assayed for the IL-8 concentration using a human IL-8 enzyme-linked immunosorbent assay (ELISA) kit according to the instructions of the manufacturer (BioLegend, San Diego, CA). IL-8 values were reported as normalized values defined as a proportion increased or decreased compared to values obtained for J99 ...
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to the staining of 332 surface antigens using LEGENDScreen Human Cell Screening PE Kit (cat.no. 700001; Biolegend, San Diego, CA, USA). Data acquisition was performed on FACSVerse (Becton Dickinson (BD) ...
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bioRxiv - Cancer Biology 2020Quote: ... We split our single cell suspension in half and stained half of the uncultured HCI010 and HCI002 cells for FACS using fluorescent labeled antibodies for human specific CD298 (Biolegend, cat. no. 341704) and mouse-specific MHC-I (eBioscience ...
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bioRxiv - Neuroscience 2022Quote: ... ELISA kit (CSB-E18033h, Generon) and monomeric α-Syn was analyzed using LEGEND MAX™ Human α-Synuclein ELISA Kit (SIG-38974, BioLegend). Then it was normalized by total protein per well using Pierce BCA Protein Assay Kit (23225 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 20 minutes on ice with 1µg of BioLegend TotalSeq-A anti-human hashtag oligo antibody (BioLegend TotalSeq-A0251 to A0258). Cells were washed 3 times then pooled at equal ratios for sequencing ...