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Citations for BioLegend :
2551 - 2600 of 4288 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-Neurod1 (1/500, mouse monoclonal, hybridoma) (Kani et al., 2010), anti-paired box 2 [Pax2] (1/700, rabbit polyclonal) (BioLegend, 901001), anti-Foxp1b ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).
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bioRxiv - Immunology 2024Quote: ... single cell suspension was incubated with the cell hashtag antibodies (unique DNA barcodes listed in Supplementary Table 2) and the cocktail of 119 unique cell surface antibodies (Biolegend, #199901), as per manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421) (BioLegend – 133911; 0.25 µg per million cells) and rat anti-mouse CD31 (labelled with AF647 ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin, PE) (BioLegend – 133906; 0.25 µg per million cells), rat anti-mouse CD31 (labelled with AF647 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were incubated for one hour at 4°C in the dark with AF647 rat anti-mouse CD326 (Epcam) antibody (BioLegend – 118212; 0.25 µg per million cells) in 200µl staining buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE) (BioLegend – 105808; 0.4 µg per million cells), rat anti-mouse CD34 (labelled with AF700 ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained in FACS buffer for 30 min at 4°C with the following anti-mouse antibodies: PacificBlue-conjugated anti-CD45 (Biolegend, clone 30-F11, #103126, 1/100), PE-Cy7-conjugated anti-CD11b (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized with 0.1% Triton X-100 in PBS (5 min at RT) and immunolabeled with anti-HA (Biolegend, 901501; 1:500 in PBS) for 1 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: C57Bl/6J or LysMGFP mice were injected intravenously with 5 µg of AF647 conjugated to anti-Ly6G antibody (127610; Biolegend, San Diego, CA USA) 3 hours before imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.0 × 106 cells were incubated at room temperature for 20 min with 5 µL of APC-labeled anti-CD45 antibody (Clone: 2D1, BioLegend, San Diego, CA, USA). The cell lines were washed in PBS containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... the FOXP3/Transcription Factor Staining Buffer kit (eBioscences – 00-5523-00) was used along with the following anti-mouse antibodies: BV605-conjugated anti-CD4 (Biolegend, RM4-5; 1/700), BV650-conjugated anti-B220 (BD Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting single cells suspension was used to detect leukocyte populations (CD45 BV421 [clone: 104; Biolegend], CD4 PerCPeFluor710 [RM4-5; eBioscience], CD8a APC-Cy7 [53-6.7; Biolegend], CD19 PE-Cy7 [6D5; Biolegend]) and CD4+ Foxp3+ Treg (CD45 BV421 ...
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bioRxiv - Genetics 2023Quote: ... The barcoded cells were then collected into a single tube and stained with a cocktail of antibodies: CD33 PECy7 (5 µL per 100 µL stain, Biolegend, cat#303434, clone WM53) and pSTAT3 AlexFlour488 (2.5 µL per 100 µL stain ...
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bioRxiv - Immunology 2019Quote: ... Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (R&D AF-400-NA goat anti-IL-1 α, 1:50; Novus Biologicals NB600-408 rabbit anti-collagen I, 1:50; Biolegend 103101 rat anti-CD45, 1:50). The next morning ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated for 30 min at 370C with 1:500 2-NBDG and 1:1000 live-dead stain Zombie NIR (Biolegend, cat.no. 423106) in cell culture media with either high or low insulin concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... and TIM-3 (F38-2E2) (Biolegend) were used to evaluate T cell phenotype ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3:20,000 anti-CD28 (BioLegend 302913), and 1:10,000 (30 IU/mL ...
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bioRxiv - Bioengineering 2021Quote: ... LAG-3 BV421 (Biolegend clone 11C3C65), PD-1 PE-Cy7 (Biolegend clone EH12.2H7).
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Galectin-3 (BioLegend, 126702), goat anti-Galectin-3 (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... anti-GATA-3/BV421 (Biolegend, USA), anti-IL-4/BV510 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... anti-Tim-3 (F38-2E2, Biolegend), anti-IFN-γ (4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... OKT-3 stock (BioLegend Inc, USA) was diluted to 50mg/ul ...